Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial Blood

Gan, Mailin; Liu, Lin; Zhang, Shunhua; Guo, Zhanbao; Tan, Yimin; Luo, Jia; Yang, Qiong; Pan, Hongmei; Li, Xuewei; Wang, Jinyong; Shen, Linyuan; Zhu, Li

doi:10.3390/ani11061563

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…Sequencing was performed on an NextSeq 500 instrument following the manufacturer’s instructions (Illumina, San Diego, CA, USA). The analysis of small RNA-seq data refers to the methods previously reported [ 41 ]. The clean reads were obtained by removing adaptors and low-quality reads.…”

Section: Methodsmentioning

confidence: 99%

Characteristics of tRNA-Derived Small RNAs and microRNAs Associated with Immunocompromise in an Intrauterine Growth-Restricted Pig Model

Gan

Chen

et al. 2022

Animals

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Intrauterine growth restriction (IUGR) is an important cause of newborn morbidity and mortality in mammals. Transfer RNA-derived small RNA (tsRNA) has become an emerging non-coding RNA in recent years. tsRNA and microRNAs (miRNAs) share similar mechanisms, which are involved in various biological processes. In this study, the pig was used as a model of IUGR, and the tsRNA and miRNA expression profile in the spleen was characterized by RNA sequencing. A total of 361 miRNAs and 620 tsRNAs were identified, of which 22 were differentially expressed miRNA (DEM) and 25 differentially expressed tsRNA (DET). tRF-5c were the primary tsRNA type making up more than 90%, and the most abundantly expressed tsRNAs are from tRNA-Gly-GCC. Functional enrichment analysis found that those DETs and DEMs have been implicated in the immune system process. Protein–protein interaction (PPI) network analysis revealed ssc-miR-370, ssc-miR-206, tiRNA-Ser-TGA-001 and tRF-Val-AAC-034 could be major regulators. TNF, TLR4, CD44, MAPK1 and STAT1 were predicted hub target genes. Those DETs and DEMs may regulate the T-cell receptor signaling pathway and Toll-like receptor signaling pathway to mediate the immunocompromise caused by IUGR. The results discussed in this article uncover the potential role of tsRNAs and miRNAs in IUGR porcine spleen.

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Section: Methodsmentioning

confidence: 99%

Characteristics of tRNA-Derived Small RNAs and microRNAs Associated with Immunocompromise in an Intrauterine Growth-Restricted Pig Model

Gan

Chen

et al. 2022

Animals

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“…The tsRNA sequencing data were analyzed as reported previously [ 38 ]. Low-quality reads and adapter sequences were removed from raw data using BOWTIE software.…”

Section: Methodsmentioning

confidence: 99%

Differential Expression Analysis of tRNA-Derived Small RNAs from Subcutaneous Adipose Tissue of Obese and Lean Pigs

Gan

Wang

Yang

et al. 2022

Animals

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Epigenetic factors, including non-coding RNA regulation, play a vital role in the development of obesity and have been well researched. Transfer RNA-derived small RNA (tsRNA) is a class of non-coding RNA proven to be involved in various aspects of mammalian biology. Here we take pigs as a model for obesity research and use tsRNA-seq to investigate the difference in tsRNA expression in the subcutaneous adipose tissue of obese and lean pigs to elucidate the role of tsRNA in obesity development. A total of 482 tsRNAs were identified in pig adipose tissue, of which 123 were significantly differentially accumulated tsRNAs compared with the control group. The tRF-5c was the main type of these tsRNAs. The largest number of tsRNAs produced was the Gly-carrying tRNA, which produced 81 tsRNAs. Functional enrichment analysis revealed that differential tsRNAs indirectly participated in MAPK, AMPK, insulin resistance, the TNF signaling pathway, adipocytokine signaling pathway, and other signaling pathways by interacting with target genes. These are involved in bioenergetic metabolic regulatory processes, suggesting that tsRNAs may influence these pathways to mediate the regulation of energy metabolism in porcine adipocytes to promote lipid deposition, thus contributing to obesity. Our findings suggest a potential function of tsRNA in regulating obesity development.

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“…The library construction and sequencing of exosomal miRNA and liver mRNA was performed by Lianchuan BioTech Co., Ltd (Lianchuan Bio, Hangzhou, China). A previous methodology was adopted to perform the RNA-seq analyses [16,17]. Differentially expressed genes (DEGs) were identified using the edgeR software (|logFC|> 1, p value < 0.05).…”

Section: Rna Extraction Library Construction Sequencing and Rt-qpcrmentioning

confidence: 99%

Cooked pork-derived exosome nanovesicles mediate metabolic disorder—microRNA could be the culprit

Shen

Yang

et al. 2023

J Nanobiotechnol

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In this study, exosomes from cooked meat were extracted by ultra-high-speed centrifugation. Approximately 80% of exosome vesicles were within 20–200 nm. In addition, the surface biomarkers of isolated exosomes were evaluated using flow cytometry. Further studies showed the exosomal microRNA profiles were different among cooked porcine muscle, fat and liver. Cooked pork-derived exosomes were chronically administered to ICR mice by drinking for 80 days. The mice plasma levels of miR-1, miR-133a-3p, miR-206 and miR-99a were increased to varying degrees after drinking exosome enriched water. Furthermore, GTT and ITT results confirmed an abnormal glucose metabolism and insulin resistance in mice. Moreover, the lipid droplets were significantly increased in the mice liver. A transcriptome analysis performed with mice liver samples identified 446 differentially expressed genes (DEGs). Functional enrichment analysis found that DEGs were enriched in metabolic pathways. Overall, the results suggest that microRNAs derived form cooked pork may function as a critical regulator of metabolic disorder in mice.

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Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial Blood

Cited by 5 publications

References 36 publications

Characteristics of tRNA-Derived Small RNAs and microRNAs Associated with Immunocompromise in an Intrauterine Growth-Restricted Pig Model

Characteristics of tRNA-Derived Small RNAs and microRNAs Associated with Immunocompromise in an Intrauterine Growth-Restricted Pig Model

Differential Expression Analysis of tRNA-Derived Small RNAs from Subcutaneous Adipose Tissue of Obese and Lean Pigs

Cooked pork-derived exosome nanovesicles mediate metabolic disorder—microRNA could be the culprit

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