Sandrine Dauchy, Florence Miller, Pierre-Olivier Couraud, Richard J. Weaver, Babette Weksler, Ignacio-Andres Romero, Jean-Michel Scherrmann, Isabelle De Waziers, Xavier Declèves
Abstract:We investigated the expression of genes encoding ABC transporters, cytochromes P450 (CYPs) and some transcription factors in the hCMEC/D3 immortalized human cerebral microvascular endothelial cell line, a promising in vitro model of the human BBB, and we compared these expressions to a non-brain endothelial cell line (HUVEC) and freshly human brain microvessels. qRT-PCR showed that the MDR1, BCRP, MRP1, MRP3, MRP4 and MRP5 genes were expressed and that the main CYP gene was CYP2U1 in hCMEC/D3. The pattern of A…
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