Expression and Significance of Hypoxia‐Inducible Factor–1α and Survivin in Laryngeal Carcinoma Tissue and Cells

Li, Dawei; Zhou, Liang; Jin, Bin; Xie, Jin; Dong, Pin

doi:10.1177/0194599812464759

Cited by 21 publications

(28 citation statements)

References 19 publications

(44 reference statements)

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“…Following a review of the literature, only 13 studies on the topic of laryngeal carcinoma and HIF were identified (Table I). These studies all examined the expression of HIF-1α only, and none investigated HIF-1α mRNA expression levels from in vivo samples (30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42). The current study is the first to present full quantitative data regarding the mRNA expression levels of HIF-1α and HIF-2α in laryngeal carcinoma samples, and is the first to report on the mRNA expression levels of HIF-3α in an in vivo study of a malignant neoplasm.…”

Section: Discussionmentioning

confidence: 99%

Relative quantitative expression of hypoxia-inducible factor-1α, −2α and −3α, and vascular endothelial growth factor A in laryngeal carcinoma

et al. 2015

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Abstract. The aim of the present study was to determine the relative quantitative expression of hypoxia-inducible factor (HIF)-1α, -2α and -3α, and VEGF-A in laryngeal carcinoma. A total of 63 patients with carcinoma of the larynx were enrolled in the study. Total RNA was isolated from fresh, frozen normal and tumor tissues of each patient, and quantitative polymerase chain reaction was performed. HIF-1α was upregulated in the majority of patients (44 patients; 69.84%). By contrast, only 7 (11.11%) patients from the whole group displayed HIF-2α overexpression, while the HIF-3α isoform was silenced in the majority of patients (48 patients, 76.19%). A small group of 5 (7.94%) patients exhibited significant overexpression of the HIF-3α isoform. VEGF-A expression was significantly higher (P<0.05) in patients with upregulated HIF-1α (2.72±1.41 RQ) compared with patients without upregulated HIF-1α (1.86±1.46 RQ). There was a moderate positive correlation between mRNA expression levels of HIF-1α and VEGF-A (r s = 0.392; P<0.005). To the best of our knowledge, this study is first to report quantitative data with regard to the expression of all three HIF isoforms in malignant neoplasms. The findings suggest the existence of specific phenotypes of HIF expression in laryngeal carcinoma, where the HIF switch is absent.

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Section: Discussionmentioning

confidence: 99%

Relative quantitative expression of hypoxia-inducible factor-1α, −2α and −3α, and vascular endothelial growth factor A in laryngeal carcinoma

et al. 2015

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“…Total RNA was extracted from the laryngeal carcinoma cells using TRIzol ® Reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocols. Subsequently, the isolated RNA was reverse-transcribed into complementary DNA (cDNA) using the PrimeScript ® RT Reagent kit (Takara Bio, Inc., Otsu, Japan), as previously described (14). The primers used were as follows: MDR1 sense, 5'-CTT CAG GGT TTC ACA TTT GGC-3' and antisense, 5'-GGT AGT CAA TGC TCC AGT GG-3'; and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) sense, 5'-CAT CTT CCA GGA GCG AGA-3' and antisense, 5'-TGT TGT CAT ACT TCT CAT-3'.…”

Section: Methodsmentioning

confidence: 99%

Effect of multidrug resistance 1/P-glycoprotein on the hypoxia-induced multidrug resistance of human laryngeal cancer cells

Zhou

Huang

et al. 2016

Oncology Letters

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Abstract. In a previous study, it was demonstrated that hypoxia upregulated the multidrug resistance (MDR) of laryngeal cancer cells to chemotherapeutic drugs, with multidrug resistance 1 (MDR1)/P-glycoprotein (P-gp) expression also being upregulated. The present study aimed to investigate the role and mechanism of MDR1/P-gp on hypoxia-induced MDR in human laryngeal carcinoma cells. The sensitivity of laryngeal cancer cells to multiple drugs and cisplatin-induced apoptosis was determined by CCK-8 assay and Annexin-V/propidium iodide staining analysis, respectively. The accumulation of rhodamine 123 (Rh123) in the cells served as an estimate of drug accumulation and was evaluated by flow cytometry (FCM). MDR1/P-gp expression was inhibited using interference RNA, and the expression of the MDR1 gene was analyzed using reverse transcription-quantitative polymerase chain reaction and western blotting. As a result, the sensitivity to multiple chemotherapeutic agents and the apoptosis rate of the hypoxic laryngeal carcinoma cells increased following a decrease in MDR1/P-gp expression (P<0.05). Additionally, FCM analysis of fluorescence intensity indicated that the downregulated expression of MDR1/P-gp markedly increased intracellular Rh123 accumulation (P<0.05). Such results suggest that MDR1/P-gp serves an important role in regulating hypoxia-induced MDR in human laryngeal carcinoma cells through a decrease in intracellular drug accumulation.

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“…6), indicating that although both HIF-1α and HIF-2α are involved in the regulation of laryngeal CSCs, HIF-1α may dominate. These findings may be helpful in explaining the relevant results from our recent study (34) where HIF-1α was highly expressed in laryngeal cancer tissues and significantly related to the clinical stage and lymph node metastasis, a possible explanation may be that advanced and metastatic cancer tissues contained a larger proportion of CSCs.…”

Section: Discussionmentioning

confidence: 55%

Hypoxia promotes stem-like properties of laryngeal cancer cell lines by increasing the CD133+ stem cell fraction

Gong

et al. 2014

International Journal of Oncology

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Evidence indicates that a hypoxic micro-environment plays an essential role in the regulation of cancer stem cells (CSCs). However, whether hypoxia is able to regulate the stem-like biological properties of laryngeal cancer cells remains unknown. In this study, we investigated the influence of hypoxia on the stemness of two laryngeal cancer cell lines, Hep-2 and AMC-HN-8. We cultured the two cell lines under hypoxia and normoxia and examined the influence of hypoxia on the expression of hypoxia-inducible factors (HIFs) and the cancer stem-like properties of these cells, including cell cycle distribution, expression of stem cell genes (OCT4, SOX2 and NANOG) and laryngeal CSC surface marker (CD133), proliferation, invasion, colony formation and sphere formation capacity. We determined that both of these cell lines, when maintained under hypoxic conditions, showed expanded cells in the G0/G1 phase, exhibited preferential expression of stem cell genes and CD133, and manifested upregulation of HIFs. When treated with hypoxia followed by normoxia exposure, the two cell lines exhibited enhanced capacities for proliferation, invasion, and sphere and colony formation compared with cells maintained consistently under normoxia. Our findings indicate that a hypoxic microenvironment may upgrade the stem-like biological properties of laryngeal cancer cell lines by the expansion of the CD133(+) stem cell fraction.

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Expression and Significance of Hypoxia‐Inducible Factor–1α and Survivin in Laryngeal Carcinoma Tissue and Cells

Abstract: HIF-1α could be considered as an important regulator for the upregulation of survivin gene expression induced by hypoxia in LSCC cells, and both proteins could be regarded as 2 key predictors of malignant progression and metastasis of LSCC.

Cited by 21 publications

References 19 publications

Relative quantitative expression of hypoxia-inducible factor-1α, −2α and −3α, and vascular endothelial growth factor A in laryngeal carcinoma

Relative quantitative expression of hypoxia-inducible factor-1α, −2α and −3α, and vascular endothelial growth factor A in laryngeal carcinoma

Effect of multidrug resistance 1/P-glycoprotein on the hypoxia-induced multidrug resistance of human laryngeal cancer cells

Hypoxia promotes stem-like properties of laryngeal cancer cell lines by increasing the CD133+ stem cell fraction

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