Expression and significance of autophagy genes LC3, Beclin1 and MMP-2 in endometriosis

Abstract

Expression of autophagy-related proteins, microtubule-associated protein light chain 3 (LC3) and Beclin1, and matrix metalloproteinase-2 (MMP-2) was investigated in serum and peritoneal fluid of patients with endometriosis (EM). The messenger ribonucleic acid (mRNA) expression levels of MMP-2, LC3 and Beclin1 in endometrial tissues of EM patients and correlation of these genes with EM and their significance were evaluated. The serum, peritoneal fluid and endometrial tissues of 84 patients treated in The First Affiliated Hospital of Qiqihar Medical University (Qiqihar, China) from March 2016 to March 2017 were collected. The serum, peritoneal fluid and endometrial tissues of 42 EM patients were used as the experimental group, while those of 42 non-EM patients were used as the control group. The levels of LC3, Beclin1 and MMP-2 in serum and peritoneal fluid of EM patients and non-EM patients were quantitatively detected via enzyme-linked immunosorbent assay (ELISA), followed by comparative analysis based on data in both groups. In addition, mRNA expression of LC3, Beclin1 and MMP-2 in the endometrium in both groups were detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and differences in expression of these genes between the groups were analyzed and evaluated. Correlation of LC3, Beclin1 and MMP-2 with EM was explored. Results of ELISA showed that levels of LC3 and Beclin1 in the EM group were significantly lower than those in the control group, while levels of MMP-2 in serum and peritoneal fluid of the EM group were significantly higher than those in the control group (P<0.05). Results of RT-qPCR revealed that mRNA expression of LC3 and Beclin1 in the endometrium of patients in the EM group were obviously decreased compared with those in the control group, while the expression of MMP-2 was high, and differences in expression were statistically significant (P<0.05). The expression of MMP-2 is high, and expression of LC3 and Beclin1 is low in serum, peritoneal fluid and endometrium of EM patients, and investigating the expression of MMP-2, LC3 and Beclin1 in EM is helpful to further clarify the pathogenesis of EM, and guide the clinical diagnosis and treatment.

Chlorogenic Acid Alleviates Thiram-Induced Tibial Dyschondroplasia by Modulating Caspases, BECN1 Expression and ECM Degradation

“…This expression variation of BECN1 and the improvement of tibial index collected from TD affected chickens in CGA groups are consistent with previous report, in which researchers point that BECN1 is important in skeletal development and benefits the mineralization of bone [30]. Moreover, BECN1 has a relation with MMPs in many diseases, such as endometriosis [31] and tongue squamous cell carcinoma [32], but the relation between them in TD still remains vague. There are several lines of evidence which show that MMPs occupy an important part in endochondral ossification through promoting angiogenesis and remodeling extracellular matrix.…”

Effect ofPeriostinSilencing on the Autophagy of Osteoblasts

“…are homologs of yeast autophagy-related genes in mammalian cells and are important genes involved in autophagy regulation (Chen et al, 2016;Jacquin et al, 2017;Kang et al, 2018). Both genes have been used as important indicators for detecting autophagy activity due to their simple detection and high specificity ( Jin et al, 2016;Kwon et al, 2017;Sui et al, 2018). The western blotting results showed that Beclin-1 protein expression was significantly higher in the experimental group than in the control group (Figs.…”

Expression and Significance of Matrix Metalloproteinase-2 and Matrix Metalloproteinas-9 in Endometriosis

“…Liu, H et al claim that plasma MMP-9 directly correlates with the severity of endometriosis and could be used as a biochemical marker [ 35 ]. However, we found only a few studies in which authors investigate MMP-2 expression in endometriosis [ 36 ].…”