Expression and regulation of versican in neural precursor cells and their lineages

Gu, Wen Li; Fu, Sai-Li; Wang, Yan Xia; Liu, Ying; Wang, Xiao Fei; Xu, Xiao–Ming; Lu, Pei-Hua

doi:10.1111/j.1745-7254.2007.00659.x

Cited by 18 publications

(20 citation statements)

References 47 publications

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“…Proteoglycans are prominently expressed in the nervous system (Gu et al 2007;Gu et al 2009;Abaskharoun et al 2010), and enzymatic digestion of chondroitin sulfate proteoglycans disrupts the development of NCSs in culture (von Holst et al 2006;Gu et al 2009). Yet, no major abnormalities have been described in the CNS of mice lacking proteoglycans, likely because of either functional redundancy or early embryonic lethality (Hartmann and Maurer 2001;Zimmermann and Dours-Zimmermann 2008).…”

Section: Proteoglycansmentioning

confidence: 99%

Extracellular Matrix: Functions in the Nervous System

Barros

Franco

Müller

2010

Cold Spring Harbor Perspectives in Biology

348

274

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An astonishing number of extracellular matrix glycoproteins are expressed in dynamic patterns in the developing and adult nervous system. Neural stem cells, neurons, and glia express receptors that mediate interactions with specific extracellular matrix molecules. Functional studies in vitro and genetic studies in mice have provided evidence that the extracellular matrix affects virtually all aspects of nervous system development and function. Here we will summarize recent findings that have shed light on the specific functions of defined extracellular matrix molecules on such diverse processes as neural stem cell differentiation, neuronal migration, the formation of axonal tracts, and the maturation and function of synapses in the peripheral and central nervous system.

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Section: Proteoglycansmentioning

confidence: 99%

Extracellular Matrix: Functions in the Nervous System

Barros

Franco

Müller

2010

Cold Spring Harbor Perspectives in Biology

348

274

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“…Versican isoform V1 promotes cell growth, aggregation and mesenchymal-epithelial transition, whereas the V2 isoform has an inhibitory effect in these processes (Schmalfeldt et al 2000;Sheng et al 2005;Sheng et al 2006;Gu et al 2007). The V2 isoform is highly expressed in the brain, predominately in white matter, with its expression increasing in the rodent brain after post-natal day (P)18 and remaining at the high level of expression seen in the adult brain (Milev et al 1998;Oohashi et al 2002).…”

Section: Research-article2015mentioning

confidence: 99%

“…In human lungs, versican accumulation is increased in many pathological conditions (Bensadoun et al 1996(Bensadoun et al , 1997Lowry et al 2008;Merrilees et al 2008;Gill et al 2010;Chang et al 2014;Wight et al 2014a). Versican expression in the CNS is also increased following injury and altered in diseases such as multiple sclerosis (Fawcett and Asher 1999;Schmalfeldt et al 2000;Sobel and Ahmed 2001;Asher et al 2002;Gu et al 2007).…”

Section: Research-article2015mentioning

confidence: 99%

“…The structure of versican is characterized by an approximately 550-kDa core protein consisting of an amino-terminal (G1) hyaluronan binding domain, a carboxy-terminal (G3) domain, and two central chondroitin sulfate glycosaminoglycan attachment domains, the α-GAG and β-GAG domains (Zimmermann and Ruoslahti 1989;Zako et al 1995;Horii-Hayashi et al 2008;Hatano et al 2012). Versican has four different isoforms that differ in the central GAG attachment domain: V0 (contains both α and β GAG domains), V1 (contains only the β domain), V2 (contains only the α domain) and V3 (devoid of both GAG domains) (Zako et al 1995;Gu et al 2007). …”

Section: Introductionmentioning

confidence: 99%

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Correlation of Versican Expression, Accumulation, and Degradation during Embryonic Development by Quantitative Immunohistochemistry

Snyder

Washington

Birkland

et al. 2015

J Histochem Cytochem.

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SummaryVersican, a chondroitin sulfate proteoglycan, is important in embryonic development, and disruption of the versican gene is embryonically lethal in the mouse. Although several studies show that versican is increased in various organs during development, a focused quantitative study on versican expression and distribution during lung and central nervous system development in the mouse has not previously been performed. We tracked changes in versican (Vcan) gene expression and in the accumulation and degradation of versican. Vcan expression and quantitative immunohistochemistry performed from embryonic day (E) 11.5 to E15.5 showed peak Vcan expression at E13.5 in the lungs and brain. Quantitative mRNA analysis and versican immunohistochemistry showed differences in the expression of the versican isoforms in the embryonic lung and head. The expression of Vcan mRNA and accumulation of versican in tissues was complementary. Immunohistochemistry demonstrated co-localization of versican accumulation and degradation, suggesting distinct roles of versican deposition and degradation in embryogenesis. Very little versican mRNA or protein was found in the lungs of 12-to 16-week-old mice but versican accumulation was significantly increased in mice with Pseudomonas aeruginosa lung infection. These data suggest that versican plays an important role in fundamental, overlapping cellular processes in lung development and infection. (J Histochem Cytochem 63:952-967, 2015)

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“…RNA Extraction and Real-Time RT-PCR mRNA expression was measured by real-time RT-PCR as described elsewhere [32]. Total RNA from injured spinal cords (1-cm spinal cord segment containing the injury epicenter) was extracted using TRI reagent (Molecular Research Center, Cincinnati, OH) according to the manufacturer's instructions.…”

Section: Passive Immunizationmentioning

confidence: 99%

Effects of Olig2-Overexpressing Neural Stem Cells and Myelin Basic Protein-Activated T Cells on Recovery from Spinal Cord Injury

Shen

Wang

et al. 2012

Neurotherapeutics

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Neural stem cell (NSC) transplantation is a major focus of current research for treatment of spinal cord injury (SCI). However, it is very important to promote the survival and differentiation of NSCs into myelinating oligodendrocytes (OLs). In this study, myelin basic protein-activated T (MBP-T) cells were passively immunized to improve the SCI microenvironment. Olig2-overexpressing NSCs were infected with a lentivirus carrying the enhanced green fluorescent protein (GFP) reporter gene to generate Olig2-GFP-NSCs that were transplanted into the injured site to differentiate into OLs. Transferred MBP-T cells infiltrated the injured spinal cord, produced neurotrophic factors, and induced the differentiation of resident microglia and/or infiltrating blood monocytes into an "alternatively activated" anti-inflammatory macrophage phenotype by producing interleukin-13. As a result, the survival of transplanted NSCs increased fivefold in MBP-T cell-transferred rats compared with that of the vehicle-treated control. In addition, the differentiation of MBP-positive OLs increased 12-fold in Olig2-GFP-NSC-transplanted rats compared with that of GFP-NSC-transplanted controls. In the MBP-T cell and Olig2-GFP-NSC combined group, the number of OL-remyelinated axons significantly increased compared with those of all other groups. However, a significant decrease in spinal cord lesion volume and an increase in spared myelin and behavioral recovery were observed in Olig2-NSC-and NSC-transplanted MBP-T cell groups. Collectively, these results suggest that MBP-T cell adoptive immunotherapy combined with NSC transplantation has a synergistic effect on histological and behavioral improvement after traumatic SCI. Although Olig2 overexpression enhances OL differentiation and myelination, the effect on functional recovery may be surpassed by MBP-T cells.

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Expression and regulation of versican in neural precursor cells and their lineages

Cited by 18 publications

References 47 publications

Extracellular Matrix: Functions in the Nervous System

Extracellular Matrix: Functions in the Nervous System

Correlation of Versican Expression, Accumulation, and Degradation during Embryonic Development by Quantitative Immunohistochemistry

Effects of Olig2-Overexpressing Neural Stem Cells and Myelin Basic Protein-Activated T Cells on Recovery from Spinal Cord Injury

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