Expression and Regulation of c-kit Receptor and Proliferative Response to Stem Cell Factor in Childhood Lymphoma and Leukemia Cells

Tomeczkowski, Jörg; Frick, D; Schwinzer, Beate; Reiter, Alfred; Welte, Karl; Sykora, Karl‐Walter

doi:10.1007/978-1-4613-0391-6_23

Cited by 5 publications

(9 citation statements)

References 51 publications

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“…[11][12][13] However, an IL-2-dependent T-lymphoblastic leukemia cell line that was stimulated to secrete GM-CSF by SCF, 37 and a case of T-ALL blast cells producing small amounts of c-kit mRNA have been described. 36 More recently, our results 38,44 have been confirmed by Kees and Ashman 45 who found c-kit expression in three of five T-ALL cell lines, and SCF had an synergistic proliferative effect with IL-2 on one of these cells. Sperling et al 39 discovered CD 117 in a small proportion of T-lineage ALL (9%) and Ichinohasama et al 46 found a c-kit expressing T-lymphoblastic lymphoma.…”

Section: Discussionsupporting

confidence: 84%

“…43 Free Dig was removed by a desalting column (Sephadex G-25). MO7e and MHH-TALL-1 cells, 5 × 10 6 cells in 100 l, were incubated with 15 l (0.13 mg/ml) Dig-rhSCF for 1 h on ice in the presence or absence of a 100-fold excess of unlabeled rhSCF.…”

Section: Labeling Of Scf and Chemical Crosslinking With Digoxigenin-rmentioning

confidence: 99%

“…6,29,35 Little is known about a direct stimulatory or inhibitory effect of SCF on childhood malignant T-lymphoblastic cells, although the expression of c-kit in T-lymphoblastic cells has been described previously. [36][37][38][39] The finding that c-kit is involved in the growth regulation of thymocytes makes this receptor a candidate for involvement in the growth and development of T-lineage leukemia and lymphoma cells. Because of the potential clinical use of SCF, we examined the expression and regulation of c-kit receptor on pediatric malignant T-lymphoblastic cells and the direct effect of SCF on these cells.…”

Section: Introductionmentioning

confidence: 99%

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Expression and regulation of c-kit receptor and response to stem cell factor in childhood malignant T-lymphoblastic cells

et al. 1998

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Section: Discussionsupporting

confidence: 84%

Section: Labeling Of Scf and Chemical Crosslinking With Digoxigenin-rmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression and regulation of c-kit receptor and response to stem cell factor in childhood malignant T-lymphoblastic cells

et al. 1998

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“…BL cells were shown to express IL-2 receptors (Matsuo et al, 1985;Wagner et al, 1987;Pang et al, 1992) and were activated to produce interferon gamma by IL-2 (Pang et al, 1992). G-CSF receptors and c-kit receptors, the receptor for stem cell factor, were absent on BL cells and could not be induced by different reagents and neither G-CSF nor SCF influenced proliferation (Tomeczkowski et al, 1995a, b). Lymphomas of different histology show varying degrees of expression of IL-1, IL-2, IL-4, IL-5, IL-6 (Emilie et al, 1993), GM-CSF and their receptors.…”

mentioning

confidence: 97%

“…The proliferative response of BL cell lines in the presence of anti-m antibodies was determined under serum-free conditions in [ 3 H]thymidine incorporation assays. All cell lines were washed three times with serum-free BL medium (Tomeczkowski et al, 1995a). The cells were then cultured at a concentration of 5 × 10 4 cells/ml in 96-well flatbottomed microtitre plates in 200 ml serum-free BL medium containing various concentrations of anti-m antibodies (Sigma, Deisenhofen, Germany).…”

Section: Fresh Lymphoma Cellsmentioning

confidence: 99%

Absence of IL‐6 receptor expression in fresh childhood Burkitt's lymphoma cells and induction of IL‐6 receptors by Epstein‐Barr virus in vitro

et al. 1997

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Summary. Interleukin-6 (IL-6) is a cytokine which is necessary for the differentiation of activated B cells and growth of early haemopoietic progenitors. It is used for exvivo expansion of myeloid progenitors in the setting of highdose chemotherapy with autologous bone marrow transplantation (BMT). Expression of the IL-6 receptor (IL-6R) was examined in six fresh Burkitt's lymphoma (BL) cell preparations and 12 BL cell lines by reverse transcriptase polymerase chain reaction (RT-PCR) and flow cytometry (FCM). Inducibility of IL-6R mRNA expression by EpsteinBarr virus (EBV) was studied by comparing two uninfected cell lines with the same cell lines infected by EBV. The phenotype of the BL cells lines was analysed by FCM and by proliferation assays in the presence of anti-IgM antibodies.None of the fresh BL cells expressed the IL-6 receptor. The BL cell lines expressed varying degrees of IL-6R mRNA and protein. In vitro infection of EBV-negative BL cell lines resulted in up-regulation of IL-6R mRNA. There was no proliferative response of the BL cell lines to IL-6, including the cells that expressed the receptor. Compared to uninfected BL cell lines, EBV-infected cell lines and lymphoblastoid cell lines (LCLs) showed a weaker or no response to anti-IgM antibodies, indicating a more mature phenotype of these cells. We conclude that the IL-6 receptor is not expressed in fresh childhood BLs, but only in BL cell lines. EBV infection in vitro leads to an up-regulation of IL-6R mRNA but not to increased proliferation. This makes growth stimulation of contaminating BL cells in the setting of autologous BMT unlikely.

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Expression of high amounts of the CD117 molecule in a case of B-cell non-Hodgkin's lymphoma carrying the t(14:18) translocation

Bravo¹,

Agustín²,

Bellas³

et al. 2000

Am. J. Hematol.

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The c‐kit proto‐oncogen (CD117) has been described to be present in normal and neoplastic hemopoietic cells including both myeloid and lymphoid lineages. Among the normal lymphoid cells CD117 expression would be restricted to a small subset of NK‐cells, and to early T‐cell precursors and it is not expressed by normal B‐cells. Regarding chronic lymphoproliferative disorders the only data provided up to now suggests that CD117 expression is restricted to cases of Hodgkin's disease and anaplastic large‐cell lymphoma. In the present paper we describe a case of a B‐cell chronic lymphoproliferative disorder carrying the t(14:18) translocation as demonstrated by molecular studies, in which the flow cytometric immunophenotypic analysis of both peripheral blood and bone marrow samples revealed the expression of high amounts of the CD117 antigen in the surface of the clonal B‐cell population. Further studies are necessary to explore both the functional role of c‐kit expression in the neoplastic B‐cells from this patient and its potential utility for the diagnosis and follow‐up of patients with B‐cell non‐Hodgkin's lymphoma. Am. J. Hematol. 63:226–229, 2000. © 2000 Wiley‐Liss, Inc.

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Expression and Regulation of c-kit Receptor and Proliferative Response to Stem Cell Factor in Childhood Lymphoma and Leukemia Cells

Cited by 5 publications

References 51 publications

Expression and regulation of c-kit receptor and response to stem cell factor in childhood malignant T-lymphoblastic cells

Expression and regulation of c-kit receptor and response to stem cell factor in childhood malignant T-lymphoblastic cells

Absence of IL‐6 receptor expression in fresh childhood Burkitt's lymphoma cells and induction of IL‐6 receptors by Epstein‐Barr virus in vitro

Expression of high amounts of the CD117 molecule in a case of B-cell non-Hodgkin's lymphoma carrying the t(14:18) translocation

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