Expression and regulation of adipocyte fatty acid binding protein in granulosa cells and its relation with clinical characteristics of polycystic ovary syndrome

Abstract

Increased expression of adipocyte fatty acid binding protein (FABP4) is associated with type 2 diabetic, high triglycerides, increased lipid peroxidation, and inflammation markers. To study the expression of FABP4 mRNA in granulosa cells of patients with polycystic ovary syndrome (PCOS) and the impact of testosterone, insulin, and PPARγ agonist rosiglitazone on granulosa cells (GCs), and to investigate the relationship of serum FABP4 levels with clinical characteristics in patients with PCOS. The expression of FABP4 mRNA in GCs of patients with PCOS and normal controls were assayed by RT-PCR. We assessed the level of FABP4 mRNA after treatment with testosterone, insulin, and rosiglitazone in GCs from normal controls. Serum FABP4 were assayed from 96 patients with PCOS (obese and nonobese 48 cases, respectively) and 80 healthy normal controls (obese and the nonobese 40 cases, respectively). The expression of FABP4 mRNA was higher in the GCs of PCOS than that of the controls (P<0.05). FABP4 mRNA expression was up-regulated by testosterone, insulin, and rosiglitazone at different dosages. Serum FABP4 levels were higher in the nonobese PCOS group than that of the nonobese controls (8.9±5.1 ng/ml vs. 4.8±0.7 ng/ml), and in the obese PCOS group than that of the obese controls (28.2±14.0 ng/ml vs. 15.6±6.6 ng/ml), respectively (P<0.05). Multiple linear regression analyses showed that serum FABP4 level was independently associated with HOMA-IR, BMI, and testosterone (P<0.05). Increased FABP4 was related to the clinical characteristics of PCOS.

Metabolomic Analysis of SCD during Goose Follicular Development: Implications for Lipid Metabolism

“…Investigations of lipid profiles in both follicular cells (including cumulus, GCs, and theca cells (TCs)) and mass spectrometry (MS) of follicular fluid suggest that lipid metabolism is pivotal for oocyte maturation and follicular development [13,14]. Moreover, as the capacity of oocytes to utilize glucose as the main energy source is limited [15], lipid metabolism in GCs of follicles is considered indispensable for oocyte maturation in cattle, sheep, and humans [16][17][18]. In a previous study, we confirmed for the first time that de novo lipogenesis occurs in goose GCs [19].…”

FASN-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis

“…As the key follicular component, granulosa cells (GCs) are essential for follicular growth, maturation, and ovulation due to their pivotal roles in regulating cellular apoptosis and steroidogenesis. In recent years, increased attention has been paid to lipid metabolism in GCs, and there are evidences that lipid metabolism in GCs is essential for maintaining ovarian follicle development in humans (Hu and Qiao, 2011) and other mammals (Campbell et al, 2010;Elis et al, 2015). Nevertheless, very little is known about the underlying mechanisms by which lipid metabolism in GCs modulates vertebrate ovarian functions.…”

Emerging concepts about prenatal genesis, aberrant metabolism and treatment paradigms in polycystic ovary syndrome

“…Genetic susceptibility loci for PCOS, identified by recent genome-wide association studies, particularly implicate genes involved in cytoplasmic function in multiple organ systems, including THADA (thyroid associated protein) [10, 11, 12] and DENND1A (DENN/MADD domain containing 1A) [10, 11]. Polymorphisms of genes involved in glucose homeostasis, including adipocyte fatty acid binding protein (FABP4) [13] and adiponectin [14], are also associated with PCOS, but PCOS-linked variants differ from those associated with type 2 DM [15]. …”