FASN-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis

Chen, Xi; Huang, Kailiang; Hu, Shiyan; Lan, Gang; Gan, Xiang; Gao, Shan‐Yan; Deng, Yan; Hu, Jiwei; Li, Liang; Hu, Bo; He, Hua; Liu, Hehe; Xia, Lu; Wang, Jiwen

doi:10.3389/fphys.2020.00600

Cited by 9 publications

(8 citation statements)

References 38 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the F1 follicle, the deposition of large amounts of yolk lipids was associated with cell proliferation and very-low-density lipoprotein transportation. Genes related to lipid metabolism were enriched at the ZT12 stage, which are essential for promoting the deposition of yolk protein and synthesis of lipids before the LH surge stage [ 46 ], For example, FASN is involved in lipid metabolism to regulate steroidogenesis in granulosa cells [ 47 ], and the observation that this gene was enriched in the ZT12 stage was consistent with the results of another study [ 48 ]. These findings suggested that cell proliferation and lipid metabolism in granulosa cells plays a pivotal role in follicle development before the LH surge stage [ 49 , 50 , 51 ].…”

Section: Discussionsupporting

confidence: 83%

Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle

Liang

Deng

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

Long non-coding RNAs (lncRNAs) and mRNAs are temporally expressed during chicken follicle development. However, follicle transcriptome studies in chickens with timepoints relating to changes in luteinizing hormone (LH) levels are rare. In this study, gene expression in Rohman layers was investigated at three distinct stages of the ovulatory cycle: zeitgeber time 0 (ZT0, 9:00 a.m.), zeitgeber time 12 (ZT12, 9:00 p.m.), and zeitgeber time 20 (ZT20, 5:00 a.m.) representing the early, middle, and LH surge stages, respectively, of the ovulatory cycle. Gene expression profiles were explored during follicle development at ZT0, ZT12, and ZT20 using Ribo-Zero RNA sequencing. The three stages were separated into two major stages, including the pre-LH surge and the LH surge stages. A total of 12,479 mRNAs and 7528 lncRNAs were identified among the three stages, and 4531, 523 differentially expressed genes (DEGs) and 2367, 211 differentially expressed lncRNAs (DELs) were identified in the ZT20 vs. ZT12, and ZT12 vs. ZT0, comparisons. Functional enrichment analysis revealed that genes involved in cell proliferation and metabolism processes (lipid-related) were mainly enriched in the ZT0 and ZT12 stages, respectively, and genes related to oxidative stress, steroids regulation, and inflammatory process were enriched in the ZT20 stage. These findings provide the basis for further investigation of the specific genetic and molecular functions of follicle development in chickens.

show abstract

Section: Discussionsupporting

confidence: 83%

Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle

Liang

Deng

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

show abstract

“…Increasing evidence has shown the importance of lipid metabolism in goose follicle development [ 30 , 31 ]; we also demonstrated that de novo lipogenesis occurs in goose GCs [ 17 ], revealing that the LD accumulation capacity of goose GCs depends on the different stages of follicle development, with the highest accumulation capacity during the preovulatory follicle stage [ 32 ]. The accumulation of LDs in the cytoplasm plays a key role in the energy balance, membrane synthesis, and cell signal transduction [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 59%

Mechanism of SCD Participation in Lipid Droplet-Mediated Steroidogenesis in Goose Granulosa Cells

Yuan

Abdul-Rahman

et al. 2022

Genes

Self Cite

View full text Add to dashboard Cite

Stearoyl-CoA desaturase (SCD) is a key enzyme catalyzing the rate-limiting step in monounsaturated fatty acids (MUFAs) production. There may be a mechanism by which SCD is involved in lipid metabolism, which is assumed to be essential for goose follicular development. For this reason, a cellular model of SCD function in goose granulosa cells (GCs) via SCD overexpression and knockdown was used to determine the role of SCD in GC proliferation using flow cytometry. We found that SCD overexpression induced and SCD knockdown inhibited GCs proliferation. Furthermore, ELISA analysis showed that SCD overexpression increased the total cholesterol (TC), progesterone, and estrogen levels in GCs, while SCD knockdown decreased TC, progesterone, and estrogen levels (p < 0.05). Combining these results with those of related multi-omics reports, we proposed a mechanism of SCD regulating the key lipids and differentially expressed gene (DEGs) in glycerophospholipid and glycerolipid metabolism, which participate in steroidogenesis mediated by the lipid droplet deposition in goose GCs. These results add further insights into understanding the lipid metabolism mechanism of goose GCs.

show abstract

“…For the overexpression group, the expression levels of FASN increased to almost 78- and 330-fold compared to the control in phGCs and hGCs, respectively. For the interference group, the expression levels of FASN decreased to 0.42- and 0.37-fold compared to the control in phGCs and hGCs, respectively [ 5 ].…”

Section: Resultsmentioning

confidence: 99%

“…GCs were isolated by 0.1% collagenase Ⅱ (Sigma, Aldrich, Burlington, MA, USA) digestion, then counted and cultured as previously described [ 26 ]. At 70~80% confluence, GCs were transfected with pEGFP-N1-FASN (2 μg/mL), pEGFP-N1 (2 μg/mL, control) and siRNA- FASN (90 pmol/mL), siRNA-NC (90 pmol/mL, control) individually using lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and assayed at 48 h post-transfection [ 5 ].…”

Section: Methodsmentioning

confidence: 99%

“…Otherwise, palmitic acid was reported to induce the apoptosis of human GCs due to its lipotoxicity [ 4 ]. Moreover, our recent research suggests that FASN interference inhibited P 4 biosynthesis of geese GCs through repressing the expression of steroidogenic acute regulatory protein ( STAR ), while FASN overexpression promoted their apoptosis due to the lipotoxicity of the product of FASN [ 5 ]. The mechanisms, however, of how FASN regulates geese GC physiological function remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Integrated Transcriptome and Metabolome Analysis Reveals the Regulatory Mechanisms of FASN in Geese Granulosa Cells

Chen

Huang

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

FASN plays a critical role in lipid metabolism, which is involved in regulating ovarian follicular development. However, the molecular mechanisms of how FASN regulate the function of ovarian follicular cells still remain elusive. In this study, by overexpression or interference of FASN in pre-hierarchical follicle granulosa cells (phGCs) and hierarchical follicle granulosa cells (hGCs), we analyzed their effects on the granulosa cell transcriptome and metabolome profiles using RNA-Seq and LC-MS/MS, respectively. The results showed that overexpression of FASN promoted proinflammatory factors expression by activating TLR3/IRF7 and TLR3/NF-κB pathways in phGCs, but only by activating TLR3/IRF7 pathways in hGCs. Then, necroptosis and apoptosis were triggered through the JAK/STAT1 pathway (induced by inflammatory factors) and BAK/caspase-7 pathway, respectively. The combined analysis of the metabolome and transcriptome revealed that FASN affected the demand of GCs for 5-hydroxytryptamine (5-HT) by activating the neuroactive ligand-receptor interaction pathway in two categorized GCs and only altering the metabolic pathway of tryptophan in phGCs, and ultimately participated in regulating the physiological function of geese GCs. Taken together, this study showed that the mechanisms of FASN regulating the physiological function of geese phGCs and hGCs were similar, but they also had some different characteristics.

show abstract

FASN-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis

Cited by 9 publications

References 38 publications

Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle

Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells during the Hen Ovulatory Cycle

Mechanism of SCD Participation in Lipid Droplet-Mediated Steroidogenesis in Goose Granulosa Cells

Integrated Transcriptome and Metabolome Analysis Reveals the Regulatory Mechanisms of FASN in Geese Granulosa Cells

Contact Info

Product

Resources

About