Expression and purification of a rapidly degraded protein, TMEM8B-a, in mammalian cell line

Wang, Li; Fan, Min; Zeng, Chao; Li, Wei; Hu, Qikang; Li, Wenliang; Huang, Xingchun; Li, Guiyuan; Yu, Fenglei

doi:10.1016/j.pep.2018.06.002

Cited by 2 publications

(2 citation statements)

References 35 publications

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“…TMEM8B is a tumor metastasis inhibitor formerly known as nasopharyngeal carcinoma associated 6 (NGX6) and was originally isolated from nasopharyngeal carcinoma [21,22] and colon cancer [23]. The TMEM8B gene acts to inhibit metastasis and restrains proliferation by arresting cell cycle progression at the G0/G1 phase of colon cancer cells in vitro [24].…”

Section: Discussionmentioning

confidence: 99%

Association of TMEM8B and SPAG8 with Mature Weight in Sheep

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Herndon

et al. 2020

Animals

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Signature of selection studies have identified many genomic regions with known functional importance and some without verified functional roles. Multiple studies have identified Transmembrane protein 8B (TMEM8B)rs426272889 as having been recently under extreme selection pressure in domesticated sheep, but no study has provided sheep phenotypic data clarifying a reason for extreme selection. We tested rs426272889 for production trait association in 770 U.S. Rambouillet, Targhee, Polypay, and Suffolk sheep. TMEM8Brs426272889 was associated with mature weight at 3 and 4 years (p < 0.05). This suggested selection for sheep growth and body size might explain the historical extreme selection pressure in this genomic region. We also tested Sperm-associated antigen 8 (SPAG8) rs160159557 encoding a G493C substitution. While this variant was associated with mature weights at ages 3 and 4, it was not as strongly associated as TMEM8Brs426272889. Transmembrane protein 8B has little functional information except as an inhibitor of cancer cell proliferation. To our knowledge, this is the first study linking TMEM8B to whole organism growth and body size under standard conditions. Additional work will be necessary to identify the underlying functional variant(s). Once identified, such variants could be used to improve sheep production through selective breeding.

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Section: Discussionmentioning

confidence: 99%

Association of TMEM8B and SPAG8 with Mature Weight in Sheep

Çınar

Mousel

Herndon

et al. 2020

Animals

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“…However, TMEM8B-a is not ubiquitinated. To this matter, Wang et al demonstrated that cell lines with stable TMEM8B-a protein expression treated with MG-132 or bortezomib could prevent TMEM8B-a degradation [ 89 ].…”

Section: Introductionmentioning

confidence: 99%

Current approach and novel perspectives in nasopharyngeal carcinoma: the role of targeting proteasome dysregulation as a molecular landmark in nasopharyngeal cancer

Yarza

Bover

Agulló-Ortuño

et al. 2021

J Exp Clin Cancer Res

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Nasopharyngeal carcinoma (NPC) represents a molecularly paradigmatic tumor given the complex diversity of environmental as well as host dependent factors that are closely implicated in tissue transformation and carcinogenesis. Epstein Barr Virus (EBV) plays a key role in tissue invasion, hyperplasia and malignant transformation. Therefore, EBV related oncoviral proteins such as Latent Membrane Protein family (LMP1, LMP2), Epstein Barr Nuclear Antigen 1 (EBNA1) and EBV related glycoprotein B (gB) are responsible for inducing intracellular signalling aberrations leading to sustained proliferation and further acquisition of NPC related invasive nature and metastatic potential.Dysregulation of proteasome signaling seems to be centrally implicated in oncoviral protein stabilization as well as in modulating tumor microenvironment. Different studies in vitro and in vivo suggest a potential role of proteasome inhibitors in the therapeutic setting of NPC. Furthermore, alterations affecting proteasome signalling in NPC have been associated to tumor growth and invasion, distant metastasis, immune exclusion and resistance as well as to clinical poor prognosis. So on, recent studies have shown the efficacy of immunotherapy as a suitable therapeutic approach to NPC. Nevertheless, novel strategies seem to look for combinatorial regimens aiming to potentiate immune recognition as well as to restore both primary and acquired immune resistance.In this work, our goal is to thoroughly review the molecular implications of proteasome dysregulation in the molecular pathogenesis of NPC, together with their direct relationship with EBV related oncoviral proteins and their role in promoting immune evasion and resistance. We also aim to hypothesize about the feasibility of the use of proteasome inhibitors as part of immunotherapy-including combinatorial regimens for their potential role in reversing immune resistance and favouring tumor recognition and eventual tumor death.

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Expression and purification of a rapidly degraded protein, TMEM8B-a, in mammalian cell line

Cited by 2 publications

References 35 publications

Association of TMEM8B and SPAG8 with Mature Weight in Sheep

Association of TMEM8B and SPAG8 with Mature Weight in Sheep

Current approach and novel perspectives in nasopharyngeal carcinoma: the role of targeting proteasome dysregulation as a molecular landmark in nasopharyngeal cancer

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