Expression and prognostic value of the Ki-67 in Wilms’ tumor: experience with 48 cases

Jurić, Ivo; Pogorelić, Zenon; Kuzmić-Prusac, Ivana; Biočić, Mihovil; Jakovljević, Gordana; Stepan, Jasminka; Župančić, Božidar; Čulić, Srđana; Krušlin, Božo

doi:10.1007/s00383-010-2588-3

Cited by 13 publications

(16 citation statements)

References 25 publications

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“…The mean proliferation index of blastemal components was 58.9±26.8 in the present study. But this ratio was lower in the other studies [10,37,38]. Both for the Ki67 and TERT staining, the epitope retrieval procedure is the most crucial step.…”

Section: Discussionmentioning

confidence: 62%

“…For example, the blastemal component had the highest proliferative activity in three studies, and the authors demonstrated that the blastemal component was a highly significant indicator of metastases and aggressive outcome in WT [29,35,36]. However in two other studies, the highest Ki67 index was determined in the epithelial component [10,36]. In the present study, we determined the highest proliferative activity in the blastemal component, and this rate was adopted as the proliferation index of the tumor.…”

Section: Discussionmentioning

confidence: 96%

“…The cure rate in these cases is close to 90% [10]. Favorable histology is characterized by the presence of all three histological elements and the absence of diffuse anaplasia [10,30]. The Wilms tumors with unfavorable histology correlate with a poor prognosis.…”

Section: Discussionmentioning

confidence: 97%

“…However, it seems that the expression of Ki67 protein is an absolute requirement for progression through the celldivision cycle [9]. Therefore, Ki67 is accepted as one of the tumor proliferation markers and is an important prognostic indicator in a variety of malignancies especially in prostate and breast carcinomas [8,10].…”

Section: Introductionmentioning

confidence: 99%

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Telomerase reverse transcriptase catalytic subunit expression and proliferation index in Wilms tumor

et al. 2011

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Telomerase activity provides telomere maintenance in chromosomes. It prevents cells from entering senescence. Telomerase activity is one of the crucial steps in various cancers. Wilms tumor (nephroblastoma) is one of the most common solid tumors of childhood. Hitherto, telomerase reverse transcriptase (TERT) catalytic subunit expression in Wilms tumor has not been investigated widely. The aim of this study was to explore the expression level of human TERT in Wilms tumor and to correlate with some clinical prognosis factors such as tumor weight, stage, histology, and Ki67 expression. This study included 41 nephroblastoma cases of childhood. The telomerase catalytic subunit expression and proliferation index was determined using an immunohistochemical method on archival paraffin-embedded tissue sections. Statistical analysis was done on SPSS 9.05 by Mann-Whitney U test and Spearman's correlation analysis. TERT expression was negative in 11 cases (26.8%), weakly positive in 14 cases (34.1%), and strongly positive in 16 cases (39%). The proliferation index was found to be 20 to 90 (mean 58.9 ± 26.8). Using Spearman correlation analysis, both the TERT expression (p=0.032) and Ki67 index (p=0.048) were found to be correlated with survival rate. Similarly, both the telomerase expression (p=0.011) and the Ki67 index (0.040) were correlated with the weight and dimension of the tumor. But there was no relationship between telomerase expression and Ki67 index (p=0.429). The mean survival time for telomerase negative cases was 56.6 ± 27.3 months, while it was 34.67 ± 28.36 months for positive cases. The Mann-Whitney U test revealed that levels of telomerase (p=0.040) significantly affected the survival rate. In the present study, we showed that the presence of TERT expression correlated with both tumor size and survival time. These findings suggest that senescence may play an important role in WT evolution, and determination of telomere maintenance will be useful to predict survival and follow-up of patients with Wilms tumor.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Telomerase reverse transcriptase catalytic subunit expression and proliferation index in Wilms tumor

et al. 2011

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“…With respect to tumor proliferative index, the three histological components of nephroblastomas (blastemal, epithelial and stromal) are known to have different proliferative potentials, with the highest proliferative index reported to occur in the epithelium 24 , 25 , 26 , 27 . The rationale behind this finding has not been fully elucidated so far, since the epithelial component is universally considered to be more differentiated than the other ones 26 , 27 .…”

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confidence: 99%

Immunohistochemical Characterization of a Renal Nephroblastoma in a <i>Trp</i>53-mutant and Prolyl Isomerase 1-deficient Mouse

et al. 2013

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A nephroblastoma is a tumor arising from metanephric blastema occurring in childhood. Among laboratory rodents, nephroblastoma has been frequently reported in rats, but it remains exceedingly rare in mice. The present work describes a nephroblastoma in a young mouse homozygous for the specific Trp53 R172H point mutation coupled with targeted deletion of the Pin1 gene. The affected kidney was effaced by a biphasic tumor with an epithelial component arranged in tubules surrounded by nests of blastemal cells. Immunohistochemically, the neoplasm was diffusely positive for Wilms’ tumor antigen. The epithelial component expressed markers of renal tubular differentiation including wide-spectrum cytokeratin, E-cadherin and folate-binding protein. Furthermore, the neoplasm exhibited a high proliferative index and diffuse nucleocytoplasmic β-catenin expression. Based on histological and immunohistochemical features, a diagnosis of nephroblastoma potentially associated with Trp53 loss and oncogenic β-catenin activation has been proposed.

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Rare localization of extrarenal nephroblastoma in 1-month-old female infant

Armanda

Čulić

Pogorelić

et al. 2012

Journal of Pediatric Urology

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Expression and prognostic value of the Ki-67 in Wilms’ tumor: experience with 48 cases

Abstract: The results support the conclusion that Ki-67 is a relevant marker for assessing the proliferative activity and tumor cell dynamics of nephroblastoma, but it may not be a good clinical prognostic marker.

Cited by 13 publications

References 25 publications

Telomerase reverse transcriptase catalytic subunit expression and proliferation index in Wilms tumor

Telomerase reverse transcriptase catalytic subunit expression and proliferation index in Wilms tumor

Immunohistochemical Characterization of a Renal Nephroblastoma in a <i>Trp</i>53-mutant and Prolyl Isomerase 1-deficient Mouse

Rare localization of extrarenal nephroblastoma in 1-month-old female infant

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