Expression and prognostic assessment of dipeptidyl peptidase IV and related enzymes in B-cell chronic lymphocytic leukemia

Sulda, Melanie; Abbott, Catherine A.; Macardle, Peter J.; Hall, Rachel; Kuss, Bryone J.

doi:10.4161/cbt.10.2.12168

Cited by 24 publications

(26 citation statements)

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“…For example, DPP9 protein levels are increased upon differentiation of monocytes to macrophages [24]. Increases in mRNa levels of DPP9 are detected in inflamed lungs and chronic lymphocytic leukemia [32,33].…”

Section: Introductionmentioning

confidence: 99%

The amino terminus extension in the long dipeptidyl peptidase 9 isoform contains a nuclear localization signal targeting the active peptidase to the nucleus

Justa-Schuch

Möller

Geiss‐Friedlander

2014

Cell. Mol. Life Sci.

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Section: Introductionmentioning

confidence: 99%

The amino terminus extension in the long dipeptidyl peptidase 9 isoform contains a nuclear localization signal targeting the active peptidase to the nucleus

Justa-Schuch

Möller

Geiss‐Friedlander

2014

Cell. Mol. Life Sci.

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“…Quantitative measurements of DPs and PEP mRNA levels were determined by real-time qPCR using TaqMan™ probe technology and external PCR product standards of known copy number. Primer and probe sequences used for DP8, DP9, DP4, FAP, DP2 and PEP are the same as previously published (37). The hypoxanthine ribosyltransferase 1 (HPRT1) house-keeping primer sequences were as previously published (50 The external PCR product standards of known number of copies of purified PCR product/ml were prepared as previously described (37).…”

Section: Rna Isolation and Real-time Quantitative Pcr (Qpcr)mentioning

confidence: 99%

“…Primer and probe sequences used for DP8, DP9, DP4, FAP, DP2 and PEP are the same as previously published (37). The hypoxanthine ribosyltransferase 1 (HPRT1) house-keeping primer sequences were as previously published (50 The external PCR product standards of known number of copies of purified PCR product/ml were prepared as previously described (37). Initial processing and analysis of the realtime qPCR data were performed using Rotorgene 6 software (Corbette Research/Qiagen).…”

Section: Rna Isolation and Real-time Quantitative Pcr (Qpcr)mentioning

confidence: 99%

“…Surface expression of endogenous DP4 and FAP protein was detected by indirect immunofluorescence staining followed by flow cytometry analysis. DP4 was detected using the anti-human CD26, clone 2A6 (mouse IgG1) (14-0269; eBioscience) and FAP detected using the F19 hybridoma supernatant as previously described (37). Alexa Fluor ® 488 goat anti-mouse (Molecular Probes ® , Invitrogen) was used as the secondary antibody.…”

Section: Rna Isolation and Real-time Quantitative Pcr (Qpcr)mentioning

confidence: 99%

“…Lysates were separated into soluble and insoluble protein fractions by centrifugation (18,000 g for 30 min at 4˚C) and protein concentration determined by Bradford assay (Bio-Rad) as per manufacturer's instructions. Soluble proteins (50 µg) were analyzed by SDS-PAGE (8%) and western blotting as previously described (37). Membranes were probed with either rabbit polyclonal anti-DP8 (RP1-DP8; Triple Point Biologics Inc., OR, USA), rabbit polyclonal anti-DP9 (RP1-DP9; Triple Point Biologics Inc.), or rabbit polyclonal anti-β-actin (ab8227; Abcam), primary antibodies diluted in blocking buffer (RP1-DP8, 1:1000; RP1-DP9, 1:2500; anti-β-actin, 1:2500).…”

Section: Rna Isolation and Real-time Quantitative Pcr (Qpcr)mentioning

confidence: 99%

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Expression profiling of dipeptidyl peptidase 8 and 9 in breast and ovarian carcinoma cell lines

Wilson

Abbott

2012

International Journal of Oncology

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Abstract. Proteases, particularly serine proteases like dipeptidyl peptidase 4 (DP4) and fibroblast activation protein (FAP), play an important role in cancer invasion and angiogenesis. Aberrant expression of DP4 and FAP is associated with numerous cancers, including breast and epithelial ovarian carcinoma. We investigated the mRNA levels, protein expression and enzyme activity of the structural homologs DP8 and DP9, in addition to DP4 and FAP, in three breast carcinoma (MDA-MB-231, MDA-MB-453, MCF-7), three epithelial ovarian carcinoma (EOC) (OVCA-432, OVCA-429, SKOV3), 293T and HeLa cell lines. In addition, DP2 and prolyl endopeptidase (PEP) mRNA and enzyme levels were measured and compared in each cell line. Ubiquitous but differential expression of DP8 and DP9 mRNA and protein was observed across all cell lines. Relative to EOC, DP8 protein was lower in the breast carcinoma cell lines (p= 0.057), suggesting that DP8 may play differing roles in different cancer cell types. A strong, negative, non-reciprocal relationship was identified between DP9 protein and DP4 mRNA (r=-0.903, p=0.002) and protein (r=-0.810, p=0.015). This suggests that DP4 expression plays an important role in the post-transcriptional regulation of DP9 in breast and ovarian cancer cell lines. Overall, this study suggests a potential role for DP8 and DP9 in breast and ovarian cancer and further investigations in this area are required. IntroductionBreast and ovarian cancer are two of the most common and lethal cancers affecting women worldwide. Estimates rank breast cancer as being the number one cause of new cancer cases (1,383,000 cases, 22.9%) and cancer related deaths (458,000 deaths, 13.7%) in women worldwide in 2008. Ovarian cancer was ranked as the seventh cause of new cancer cases (225,000 cases, 3.7%) and cancer related deaths (140,000 deaths, 4.2%) in women worldwide in the same year (1). Among gynecological malignancies, epithelial ovarian carcinoma (EOC) is the most lethal to women in the world, accounting for approximately 90% of all tumors affecting the ovaries (2). Mortality from breast cancer often results from distant metastatic spread to lung, bone and lymph node tissue while diagnosis of EOC typically occurs in the late stages of disease after its spread into the peritoneal cavity or other distant sites (2), thus making it difficult for effective treatment to be administered. Although a number of risk factors for the development of breast and ovarian cancer have been identified the exact origin and pathogenesis of disease is still poorly understood (3,4). Increasing our knowledge about the fundamental biology of these diseases is needed for the development of improved diagnostic, prognostic and therapeutic interventions at all stages of disease.Enzymatic members of the DP4-like gene family, DP4, FAP, DP8 and DP9, share the rare ability to cleave N-terminal dipeptides at post-proline bonds in the penultimate position and play an important role in the biological processing of the N-termini of peptides such as chemokines, glu...

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Targeting dipeptidyl peptidase 8 genes inhibits proliferation, migration and invasion by inhibition of cyclin D1 and MMP2MMP9 signal pathway in cervical cancer

Chen

Liu

et al. 2018

The Journal of Gene Medicine

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Expression and prognostic assessment of dipeptidyl peptidase IV and related enzymes in B-cell chronic lymphocytic leukemia

Cited by 24 publications

References 50 publications

The amino terminus extension in the long dipeptidyl peptidase 9 isoform contains a nuclear localization signal targeting the active peptidase to the nucleus

The amino terminus extension in the long dipeptidyl peptidase 9 isoform contains a nuclear localization signal targeting the active peptidase to the nucleus

Expression profiling of dipeptidyl peptidase 8 and 9 in breast and ovarian carcinoma cell lines

Targeting dipeptidyl peptidase 8 genes inhibits proliferation, migration and invasion by inhibition of cyclin D1 and MMP2MMP9 signal pathway in cervical cancer

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