Targeting dipeptidyl peptidase 8 genes inhibits proliferation, migration and invasion by inhibition of cyclin D1 and MMP2MMP9 signal pathway in cervical cancer

Chen, Yurou; Liu, Fulin; Wu, Kejia; Wu, Wanrong; Wu, Hailing; Zhang, Wei

doi:10.1002/jgm.3056

Cited by 12 publications

(11 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…c-Myc has a number of putative targets, including genes involved in cell cycle control, apoptosis, DNA metabolism and dynamics along with energy metabolism and macromolecular synthesis[15]. CyclinD1 is responsible for cell cycle progression in the transition from G0/G1 to S phase and is overexpressed in various cancers such as cervical cancer [21]. The C-MYC and CyclinD1 were also identified as target genes in Wnt/β-catenin signaling conducted in the human HT29 colorectal cancer cell line harboring mutant APC alleles using a differential RNA expression screen [22].…”

Section: Discussionmentioning

confidence: 99%

Knock-Down of HOXB8 Prohibits Proliferation and Migration of Colorectal Cancer Cells via Wnt/β-Catenin Signaling Pathway

Lin

Jiang

et al. 2019

Med Sci Monit

View full text Add to dashboard Cite

BackgroundThere has been no research on the mechanism of HOXB8 action on colorectal cancer so far. This study was designed to investigate the mechanism of HOXB8 regulating colorectal cancer cell proliferation and invasion in vivo and in vitro.Material/MethodsHOXB8 shRNA, HOXB8 overexpression, and negative control vector were designed and stably transfected into HCT116 cells. MTT assays were performed to detect cell proliferation. Western blot was utilized to detect HOXB8 expression level in HCT116 stable cells. The invasive and migration abilities were detected by Transwell assay and wound-healing assay.ResultsHOXB8 knockdown inhibited cell proliferation. The invasiveness of HCT116 cells was significantly reduced following HOXB8 depletion compared with that in the shRNA control group, whereby the rates were reduced by 67% in HOXB8 knockdown group. The wound-healing rate of HOXB8 over-expression cells was significantly increased comparing with that of cells in the blank control group (P<0.05). HOXB8 knockdown promotes apoptosis of HCT116 cells. The expression of E-cadherin was restrained in the HOXB8 over-expression group and increased in the HOXB8 knockdown group.ConclusionsKnock-down of HOXB8 prohibits the proliferation and migration of colorectal cancer cells via the Wnt/β-catenin signaling pathway and the downregulation of various factors, such as MMP2, c-Myc, CyclinD1,and vimentin. Our data suggested that HOXB8 has great potential to be developed as a novel therapeutic agent for the treatment of human colorectal cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Knock-Down of HOXB8 Prohibits Proliferation and Migration of Colorectal Cancer Cells via Wnt/β-Catenin Signaling Pathway

Lin

Jiang

et al. 2019

Med Sci Monit

View full text Add to dashboard Cite

show abstract

“…Studies in recent years have further proved that DPP8/9 is involved in the occurrence and development of various tumors, and their potential mechanisms have been explored. For example, inhibition of DPP8 expression in cervical cancer cells can inhibit cell proliferation, migration, and invasion by affecting the expression of Bax and BCL2, inhibiting the expression of MMP2 and MMP9 ( Chen et al, 2018 ). DPP9 affects the adhesive migration of tumor cells via colocalizing with cytoskeletal proteins ( Zhang et al, 2015b ).…”

Section: Discussionmentioning

confidence: 99%

“…These findings suggest that DPP9 may be involved in tumor metastasis. Moreover, it was found that knockdown of DPP8 in HeLa and SiHa cells also inhibited the expression of MMP2 and MMP9, reducing cell migration and invasion ( Chen et al, 2018 ).…”

Section: Biological Activity and Physiological Functions Of Dpp8/9mentioning

confidence: 99%

“…Moreover, DPP8 has also been indicated to participate in the pathogenesis of cervical cancer ( Huo et al, 2016 ). After silencing DPP8 in human cervical cancer cell lines, G1 phase arrest increased Bax expression, decreased BCL2 expression, inhibited cell proliferation, promoted cell apoptosis, and inhibited the expression of MMP2 and MMP9, reducing cell migration and invasion ( Chen et al, 2018 ). The expression of DPP8/9 was also detected in the reproductive system of male animals, but the specific role is not clear so far.…”

Section: Dpp8/9 and Diseasesmentioning

confidence: 99%

See 1 more Smart Citation

New insights into the role of dipeptidyl peptidase 8 and dipeptidyl peptidase 9 and their inhibitors

et al. 2022

View full text Add to dashboard Cite

Dipeptidyl peptidase 8 (DPP8) and 9 (DPP9) are widely expressed in mammals including humans, mainly locate in the cytoplasm. The DPP8 and DPP9 (DPP8/9) belong to serine proteolytic enzymes, they can recognize and cleave N-terminal dipeptides of specific substrates if proline is at the penultimate position. Because the localization of DPP8/9 is different from that of DPP4 and the substrates for DPP8/9 are not yet completely clear, their physiological and pathological roles are still being further explored. In this article, we will review the recent research advances focusing on the expression, regulation, and functions of DPP8/9 in physiology and pathology status. Emerging research results have shown that DPP8/9 is involved in various biological processes such as cell behavior, energy metabolism, and immune regulation, which plays an essential role in maintaining normal development and physiological functions of the body. DPP8/9 is also involved in pathological processes such as tumorigenesis, inflammation, and organ fibrosis. In recent years, related research on immune cell pyroptosis has made DPP8/9 a new potential target for the treatment of hematological diseases. In addition, DPP8/9 inhibitors also have great potential in the treatment of tumors and chronic kidney disease.

show abstract

“…Recent studies have shown that DPP9 is involved in cancer, inflammation, and immune diseases [7,[9][10][11]. Inhibition of DPP9 activity has been demonstrated to reduce tumor growth and metastasis in animal models of cancer [12,13]. In addition, inhibition of DPP9 has been shown to decrease T-cell proliferation and cytokine production in animal models of autoimmune diseases [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Hippocampal Dipeptidyl Peptidase 9 Bidirectionally Regulates Memory Via Synaptic Plasticity

Zhao,

Wang,

Guo

et al. 2023

Preprint

View full text Add to dashboard Cite

Dipeptidyl Peptidase family members are known to modulate memory through peripheral substrates. However, the direct impact of this family on the central nervous system (CNS) remains underexplored. Dipeptidyl peptidase 9 (DPP9) has been extensively studied for its role in inflammation and cancer. Here, we found that DPP9 is relevant highly expressed in hippocampal neurons. Electrophysiological and behavioral experiments have shown that it plays a pivotal role in long-term potentiation and memory. Proteomics analysis identified differentially expressed proteins associated with memory retrieval regulated by DPP9. Further investigation revealed that DPP9 enzymatic activity is essential for memory retrieval. DPP9-interacting proteins are involved in the function of the dendritic spine and axon, and two memory- and axon-related genes, Tmp3 and Baiap2, were identified as potential targets for DPP9. Our findings suggest that DPP9 is a novel memory regulatory protein and provide new insights into memory molecular mechanisms.

show abstract

Targeting dipeptidyl peptidase 8 genes inhibits proliferation, migration and invasion by inhibition of cyclin D1 and MMP2MMP9 signal pathway in cervical cancer

Abstract: The data obtained in the present study reveal that DPP8 promotes the progression of cervical cancer.

Cited by 12 publications

References 30 publications

Knock-Down of HOXB8 Prohibits Proliferation and Migration of Colorectal Cancer Cells via Wnt/β-Catenin Signaling Pathway

Knock-Down of HOXB8 Prohibits Proliferation and Migration of Colorectal Cancer Cells via Wnt/β-Catenin Signaling Pathway

New insights into the role of dipeptidyl peptidase 8 and dipeptidyl peptidase 9 and their inhibitors

Hippocampal Dipeptidyl Peptidase 9 Bidirectionally Regulates Memory Via Synaptic Plasticity

Contact Info

Product

Resources

About