Expression and prognosis analyses of the Tob/BTG antiproliferative (APRO) protein family in human cancers

Bai, Yuxiang; Liao, Qiao; Xie, Ning; Shi, Yongquan; Liu, Na; Wang, Jinhai

doi:10.1371/journal.pone.0184902

Cited by 24 publications

(23 citation statements)

References 43 publications

(51 reference statements)

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“…Recently, analyses based on large accessible databases have gradually become the hottest scientific research method. These analyses have led to the discovery of new prognostic indexes such as S100 family members, STATs, and the APRO protein family in breast cancer 3–5. There may also be new gene families that are associated with the prognosis of breast cancer but have not yet been detected.…”

Section: Introductionmentioning

confidence: 99%

<p>Significant prognostic values of aquaporin mRNA expression in breast cancer</p>

Zhu¹,

Ma²,

Wang³

et al. 2019

CMAR

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Introduction Aquaporins (AQPs), also called water channels, have been shown to have functions in the migration, invasion, and proliferation of human breast tumor cells. Most AQP mRNA expression levels were tested by cell lines, mouse models, and even human breast tissues. However, the mRNA expression of individual AQPs in different clinicopathologic characteristics and prognostic values according to different kinds of classifications of breast cancer patients remains unclear. Materials and methods In the current study, we used the Oncomine database, Breast cancer Gene-Expression Miner v4.1, Kaplan–Meier Plotter, and cBioPortal to investigate the expression distribution and prognostic values of AQPs in breast cancer patients. Results Our study revealed that the mRNA expression levels of AQP8, AQP9, and AQP10 were upregulated, while those of AQP3, AQP4, AQP5, and AQP7 were downregulated in breast cancer. The clinical database showed that lower mRNA levels of AQP1 were associated with a high Scarff–Bloom–Richardson grade, but AQP9 showed the opposite trend. Further survival analyses indicated that high mRNA expression levels of AQP0, AQP1, AQP2, AQP4, AQP6, AQP8, AQP10, and AQP11 were significantly associated with better relapse-free survival (RFS). Conversely, AQP3 and AQP9 were associated with worse RFS in breast cancer patients, suggesting that these two genes might be potential targets in future chemotherapy. Discussion These significant AQP members might be further explored as new biomarkers for breast cancer prognosis, but this needs further study.

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Section: Introductionmentioning

confidence: 99%

<p>Significant prognostic values of aquaporin mRNA expression in breast cancer</p>

Zhu¹,

Ma²,

Wang³

et al. 2019

CMAR

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“…It has been reported that BTG1 acts as a tumor suppressor in several human malignant tumors, including CRC (38). It is also known to be a gene that induces apoptosis and inhibits proliferation (23).…”

Section: Resultsmentioning

confidence: 99%

“…RIPA lysis buffer (Beyotime Institute of Biotechnology) was used for cell lysis, the protein concentration was determined using a bicinchoninic acid assay (Thermo Fisher Scientific, Inc.) and 10% SDS-PAGE was used to separate cellular proteins (50 µg/lane). Subsequently, protein was transferred to a 0.45 µm PVDF filter at 200 V for 2 h. The subsequent western blot analysis was carried out as described previously (38). Following blocking in 3% BSA (A8020; Beijing Solarbio Science & Technology) for 2 h at room temperature, membranes were incubated with appropriate primary antibodies in dilution buffer (3% BSA; 1:1,000 dilution) overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

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miR‑27a‑3p regulates proliferation and apoptosis of colon cancer cells by potentially targeting BTG1

Huang

Liu

et al. 2019

Oncol Lett

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microRNA (miR/miRNA)-27a-3p has been reported to be abnormally expressed in various types of cancer, including colorectal cancer (CRC). B-cell translocation gene 1 (BTG1) has also been implicated with CRC. However, the association between miR-27a-3p and BTG1 in CRC, to the best of our knowledge, has not been investigated. In order to assess whether miR-27a-3p is associated with CRC, reverse transcription-quantitative PCR was performed on 20 paired CRC and paracancerous tissues for miRNA analysis. For the screening and validation of miR-27a-3p expression in colon cancer, several colon cancer cell lines (HCT-116, HCT8, SW480, HT29, LOVO and Caco2) and the normal colorectal epithelial cell line NCM460 were examined. The highest expression levels of miR-27a-3p were detected in the HCT-116, which was selected for further experimentation. The HCT-116 cells were divided into control, miR-27a-3p mimic and inhibitor groups, and cell proliferation was tested using an MTT assay. Additionally, miR-27a-3p inhibitor/mimic or BTG1 plasmid were transfected into the HCT-116 cells, and flow cytometry was performed to analyze cell cycle distributions. TUNEL analysis was performed to detect apoptosis. Protein levels of factors in the downstream signaling pathway mediated by miR-27a-3p [ERK/mitogen-activated extracellular signal-regulated kinase (MEK)] were detected. miR-27a-3p was revealed to be overexpressed in human CRC tissues and colon cancer cell lines. Knockdown of miR-27a-3p suppressed proliferation of HCT-116 cells and apoptosis was increased. It further markedly upregulated expression levels of BTG1 and inhibited activation of proteins of the ERK/MEK signaling pathway. In addition, overexpression of BTG1 in HCT-116 cells triggered G 1 /S phase cell cycle arrest and increased apoptosis via the ERK/MEK signaling pathway. In conclusion, the present study demonstrated that the effects of miR-27a-3p on colon cancer cell proliferation and apoptosis were similar to those of the tumor suppressor gene BTG1. The miR-27a-3p/BTG1 axis may have potential implications for diagnostic and therapeutic approaches in CRC.

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“…BTG-1 is a member of the BTG/Tob antiproliferation gene family 27 , 28 and has been shown to block the rate of cell proliferation 29 , 30 by modulating the cell cycle distribution. For example, when cells are in the G 0 /G 1 phase, BTG-1 expression is high, which can promote neovascularization and cell differentiation 31 .…”

Section: Discussionmentioning

confidence: 99%

Plasma exosome-derived B-cell translation gene 1: a predictive marker for the prognosis in patients with non-small cell lung cancer

Wan¹,

Chen²,

Deng³

et al. 2021

J. Cancer

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Expression and prognosis analyses of the Tob/BTG antiproliferative (APRO) protein family in human cancers

Cited by 24 publications

References 43 publications

<p>Significant prognostic values of aquaporin mRNA expression in breast cancer</p>

<p>Significant prognostic values of aquaporin mRNA expression in breast cancer</p>

miR‑27a‑3p regulates proliferation and apoptosis of colon cancer cells by potentially targeting BTG1

Plasma exosome-derived B-cell translation gene 1: a predictive marker for the prognosis in patients with non-small cell lung cancer

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