Expression and localization of rat NBC4c in liver and renal uroepithelium

Abuladze, Natalia; Pushkin, Alexander; Tatishchev, Sergei; Newman, Debra K.; Sassani, Pejvak; Kurtz, Ira

doi:10.1152/ajpcell.00590.2003

Cited by 41 publications

(27 citation statements)

References 73 publications

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“…These findings expand the biologic role of NBCe2 in CPE cells beyond merely its transport function and suggest that NBCe2 is involved in the modeling of CPE cells in vivo. We have previously reported that NBCe2 is expressed in the mammalian kidney and liver (36). In NBCe2 Ϫ/Ϫ mice, the liver and kidney were grossly and histologically normal, as were the plasma electrolytes (not shown), suggesting that additional compensatory processes are present.…”

Section: Discussionmentioning

confidence: 86%

Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene)

Kao

Kurtz

Shao

et al. 2011

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 86%

Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene)

Kao

Kurtz

Shao

et al. 2011

Journal of Biological Chemistry

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“…Immunohistochemistry and immuno-electron microscopy revealed that NBC4 is localized to the apical brush border membrane where Na + /K + -ATPase localizes [21]. The polarized membrane localization of NBC4c has also been reported by immunohistology: NBC4c is localized to the basolateral membranes of hepatocytes and renal pelvic uroepithelium and to the apical membrane of the choroid plexus epithelial cells [11] and intrahepatic cholangiocytes [22]. However, the mechanism for the specific-membrane targeting of NBC4c and NBC4g remains unknown.…”

Section: Nbc4g Is An Apical Hco 3 − Transportermentioning

confidence: 86%

Identification and properties of a novel variant of NBC4 (Na+/HCO3− co-transporter 4) that is predominantly expressed in the choroid plexus

Fukuda

Hirata

Nakamura

et al. 2013

Biochemical Journal

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Secretion of HCO(3)- at the apical side of the epithelial cells of the choroid plexus is an essential step in the formation of cerebrospinal fluid. Anion conductance with a high degree of HCO(3)- permeability has been observed and suggested to be the major pathway for HCO(3)- transport across the apical membrane. Recently, it was found that NBC (Na(+)/HCO(3)- co-transporter) 4, an electrogenic member of the NBC family, was expressed in the choroid plexus. We found that a novel variant of the NBC4 [NBC4g/Slc4a5 (solute carrier family 4, sodium bicarbonate co-transporter, member 5)] is almost exclusively expressed in the apical membrane of rat choroid plexus epithelium at exceptionally high levels. RNA interference-mediated knockdown allowed the functional demonstration that NBC4g is the major player in the HCO(3)- transport across the apical membrane of the choroid plexus epithelium. When combined with a recent observation that in choroid plexus epithelial cells electrogenic NBC operates with a stoichiometry of 3:1, the results of the present study suggest that NBC4g mediates the efflux of HCO(3)- and contributes to cerebrospinal fluid production.

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“…The acidic environment of the excurrent ducts maintains sperm in an immotile but viable state while they mature and are stored in the epididymis. In the digestive tract, CAIX is expressed (1,26,54) in basolateral membranes of gall bladder epithelial cells, hepatic biliary duct cells, and pancreatic duct cells, each of which secretes HCO 3 Ϫ -rich luminal fluid. Several candidate bicarbonate transporters are coexpressed with CAIX in these digestive tract epithelial cells (35).…”

Section: C745mentioning

confidence: 99%

Interactions of transmembrane carbonic anhydrase, CAIX, with bicarbonate transporters

Morgan

Pastoreková²,

Stuart-Tilley³

et al. 2007

American Journal of Physiology-Cell Physiology

130

120

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Association of some plasma membrane bicarbonate transporters with carbonic anhydrase enzymes forms a bicarbonate transport metabolon to facilitate metabolic CO(2)-HCO(3)(-) conversions and coupled HCO(3)(-) transport. The transmembrane carbonic anhydrase, CAIX, with its extracellular catalytic site, is highly expressed in parietal and other cells of gastric mucosa, suggesting a role in acid secretion. We examined in transfected HEK293 cells the functional and physical interactions between CAIX and the parietal cell Cl(-)/HCO(3)(-) exchanger AE2 or the putative Cl(-)/HCO(3)(-) exchanger SLC26A7. Coexpression of CAIX increased AE2 transport activity by 28 +/- 7% and also activated transport mediated by AE1 and AE3 (32 +/- 10 and 37 +/- 9%, respectively). In contrast, despite a transport rate comparable to that of AE3, coexpressed CAIX did not alter transport associated with SLC26A7. The CAIX-associated increase of AE2 activity did not result from altered AE2 expression or cell surface processing. CAIX was coimmunoprecipitated with the coexpressed SLC4 polypeptides AE1, AE2, and AE3, but not with SLC26A7. GST pull-down assays with a series of domain-deleted forms of CAIX revealed that the catalytic domain of CAIX mediated interaction with AE2. AE2 and CAIX colocalized in human gastric mucosa, as indicated by coimmunofluorescence. This is the first example of a functional and physical interaction between a bicarbonate transporter and a transmembrane carbonic anhydrase. We conclude that CAIX can bind to some Cl(-)/HCO(3)(-) exchangers to form a bicarbonate transport metabolon.

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Expression and localization of rat NBC4c in liver and renal uroepithelium

Abstract: Abuladze, Natalia, Alexander Pushkin, Sergei Tatishchev, Debra Newman, Pakan Sassani, and Ira Kurtz. Expression and localization of rat NBC4c in liver and renal uroepithelium.

Cited by 41 publications

References 73 publications

Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene)

Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene)

Identification and properties of a novel variant of NBC4 (Na+/HCO3− co-transporter 4) that is predominantly expressed in the choroid plexus

Interactions of transmembrane carbonic anhydrase, CAIX, with bicarbonate transporters

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