Expression and functional role of Bone Morphogenetic Proteins (BMPs) in cyclical corpus luteum in buffalo ( Bubalus bubalis )

Rajesh, G.; Paul, Avishek; Mishra, Sumit; Bharati, Jaya; Thakur, Neeraj; Mondal, Tanmay; Soren, Sanjhali; Harikumar, S.; Narayanan, K.; Chouhan, V.S.; Bag, Sadhan; Das, Bikash; Singh, Gyanendra; Maurya, V. P.; Sharma, G. Taru; Sarkar, Mihir

doi:10.1016/j.ygcen.2016.10.016

Cited by 13 publications

(12 citation statements)

References 39 publications

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“…However, mRNA for BMP7, which inhibits ovulation and progesterone synthesis in rats (Lee et al, 2001), was undetectable in our study. It is unlikely that BMP7 is involved in luteolysis because, to our knowledge, there is only one report of greater BMP7 expression in the early stages of CL in buffalo (Rajesh et al, 2017) and the same authors demonstrated a positive effect of BMP7 on P4 secretion and mRNA expression of steroidogenic enzymes and pro-survival factors.…”

Section: Discussionmentioning

confidence: 86%

“…The higher levels of BMPR1B observed during CL regression in rats suggests its implication in luteal degradation (Erickson and Shimasaki, 2003). In disagreement with this hypothesis, BMPR1A, BMPR1B and BMPR2 mRNAs were positively regulated during the intermediate luteal phase in buffaloes (between days 5 and 10), compared to other luteal stages (Rajesh et al, 2017). Unlike BMPRs, TGFBR3 (betaglycan) does not participate in BMPs signaling directly, being considered a reservoir of ligands.…”

Section: Discussionmentioning

confidence: 97%

“…It has also been demonstrated that some bone morphogenetic proteins (BMPs) and their receptors are more expressed in the CL of women during spontaneous regression, and are negatively regulated by the luteotropic hormone hCG (human chorionic gonadotropin) (Nio-Kobayashi et al, 2015). In contrast to the well-established involvement in folliculogenesis, few studies (Erickson and Shimasaki, 2003;Nio-Kobayashi et al, 2015;Rajesh et al, 2017) have investigated the regulation and function of BMPs during luteinization and luteolysis.…”

Section: Introductionmentioning

confidence: 99%

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Transforming growth factor-beta family members are regulated during induced luteolysis in cattle

et al. 2019

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The transforming growth factors beta (TGFβ) are local factors produced by ovarian cells which, after binding to their receptors, regulate follicular deviation and ovulation. However, their regulation and function during corpus luteum (CL) regression has been poorly investigated. The present study evaluated the mRNA regulation of some TGFβ family ligands and their receptors in the bovine CL during induced luteolysis in vivo. On day 10 of the estrous cycle, cows received an injection of prostaglandin F2α (PGF) and luteal samples were obtained from separate groups of cows (n= 4-5 cows per time-point) at 0, 2, 12, 24 or 48 h after treatment. Since TGF beta family comprises more than 30 ligands, we focused in some candidates genes such as activin receptors (ACVR-1A, -1B, -2A, -2B) AMH, AMHR2, BMPs (BMP-1, -2, -3, -4, -6 and -7), BMP receptors (BMPR-1A, -1B and -2), inhibin subunits (INH-A, -BA, -BB) and betaglycan (TGFBR3). The mRNA levels of BMP4, BMP6 and INHBA were higher at 2 h after PGF administration (P<0.05) in comparison to 0 h. The relative mRNA abundance of BMP1, BMP2, BMP3, BMP4, BMP6, ACVR1B, INHBA and INHBB was upregulated up to 12 h post PGF (P<0.05). On the other hand, TGFBR3 mRNA that codes for a reservoir of ligands that bind to TGF-beta receptors, was lower at 48 h. In conclusion, findings from this study demonstrated that genes encoding several TGFβ family members are expressed in a time-specific manner after PGF administration.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Transforming growth factor-beta family members are regulated during induced luteolysis in cattle

et al. 2019

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“…In the mammalian ovary, BMP7 is highly expressed in the GCs and theca cells (TCs) of dominant follicles [28]. In follicular cells cultured in vitro, the inhibition of BMP7 induced the suppression of androgen secretion by bovine ovarian TCs [29], whereas the addition of recombinant BMP7 stimulated estrogen (E2) release by buffalo ovarian GCs [28], as well as progesterone (P4) production by human ovarian granulosa lutein cells [30], and buffalo ovarian luteal cells [31]. BMP7 also promoted GC survival in the buffalo ovary [28] and GC proliferation in the rat ovary [32].…”

Section: Discussionmentioning

confidence: 99%

Variants in BMP7 and BMP15 3’-UTRs Associated with Reproductive Traits in a Large White Pig Population

Yin

Pan

et al. 2019

Animals

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Bone morphogenetic protein 7 (BMP7) and BMP15, which encode members of the BMP family, have been identified by whole-genome resequencing as breeding-related genes that overlap with a known quantitative trait locus for reproductive traits. In this study, we investigated the effects of variants at the BMP7 and BMP15 gene loci on sow reproductive traits. We isolated 669 and 1213 bp sequences of the 3’-untranslated region (3’-UTR) of the porcine BMP7 and BMP15 genes, respectively, and detected several RNA regulatory elements, such as miRNA response elements and AU-rich elements. Pooled DNA sequencing identified two novel point mutations (viz., BMP7 c.1569A>G and BMP15 c.2366G>A) in the 3’-UTR. Association analysis showed that the c.1569A>G polymorphism was associated with the litter weight trait in a Large White pig population. Furthermore, analysis of the combined genetic effects revealed that AA/GA and AG/GG were the favorable combined genotypes for the total number of piglets born (TNB) and the total number of piglets born alive (NBA), whereas. Together, our findings confirm that BMP7 and BMP15 are candidate genes for porcine reproductive performance.

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“…primers EGR 1, EGR 2, EGR 3, EGR 4, AKT, BAX, StAR, 3βHSD and CYP11A1 were designed using DNAStar (online trial version, DNASTARLasergene 6, 2004), Gene Tool (online trial version, 2004) and Oligo Analyser 3.1 (open access tool, 2017) software. Published primers were used for 40 S ribosomal protein S15 (RPS15A)44 and vWF46 . Details of the primers used are presented inTable 1.…”

mentioning

confidence: 99%

Early growth response gene mediates in VEGF and FGF signaling as dissected by CRISPR in corpus luteum of water buffalo

Punetha

Chouhan

Sonwane

et al. 2020

Sci Rep

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The EGR family comprises of EGR 1, EGR 2, EGR 3 and EGR 4 which are involved in the transactivation of several genes. A broad range of extracellular stimuli by growth factors is capable of activating eGR mediated transactivation of genes involved in angiogenesis and cell proliferation. However, their role in controlling VEGF A and FGF 2 signaling in the CL of water buffalo is not known. The present study was conducted to understand the role of EGR mediated regulation of VEGF A and FGF 2 signaling in buffalo luteal cells. Towards this goal, luteal cells were cultured and treated with VEGF A and FGF 2 and the mRNA expression pattern of EGR family members were documented. The EGR 1 message was found to be up-regulated in luteal cells of buffalo at 72 hours of culture. The functional validation of EGR 1 gene was accomplished by knocking out (KO) of EGR 1 in cultured luteal cells by CRISPR/Cas9 mediated gene editing technology. The EGR 1 KO cells were then cultured and stimulated with VEGF A and FGF 2. It was observed that VEGF A and FGF 2 induced angiogenesis, cell proliferation and steroidogenesis in wild type luteal cells, whereas the response of the growth factors was attenuated in the EGR 1 KO cells. taken together our study provides evidence convincingly that both VeGf and fGf mediate their biological action through a common intermediate, EGR 1, to regulate corpus luteum function of buffalo.The corpus luteum (CL) is essential for the pregnancy establishment and its maintenance as it primarily secretes progesterone 1 . Luteal dysfunction in domestic animals results in inadequate progesterone production, poor embryonic development, infertility and increased pregnancy failure in buffalo cow 2 . Although, LH and PGF2α plays important role in development and regression of corpus luteum, but there are several other ovarian peptides which are critical for development, function and regression of CL. These include fibroblast growth factors (FGF) 3 , the vascular endothelial growth factor (VEGF) 4 , thrombospondins (TSPs) 5 , IGF 6 , Leptin 7 , Angiopoietin 8 and Ghrelin 9 . Among these VEGF helps in the regulation of angiogenesis, vasculogenesis, steroidogenesis of the CL in several species such as cattle 10 , pigs 11 , and buffaloes 12 . The transition of follicular cell to luteal cell, angiogenesis in CL and its function is also controlled by FGF 2 in cattle 13 and buffalo 3 . Growth factors such as VEGF and FGF family mediate their physiological role by binding with their transmembrane tyrosine kinase receptor. The complex dimerizes and stimulates the receptor's intrinsic tyrosine kinase activity. Ligand activation of Receptor tyrosine kinase (RTK) activates downstream pathways such as mitogen-activated protein kinase (MAPK), protein kinase B (PKB)/ phosphatidylinositol-3-kinase (PI3K) and protein kinase C (PKC) in a ligand and cell-context manner 14 . The downstream signaling further phosphorylates the dual acceptor motif ERK1/2. Activated ERK translocates into the nucleus and promotes binding SRF/Elk-1 which subsequ...

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Expression and functional role of Bone Morphogenetic Proteins (BMPs) in cyclical corpus luteum in buffalo ( Bubalus bubalis )

Cited by 13 publications

References 39 publications

Transforming growth factor-beta family members are regulated during induced luteolysis in cattle

Transforming growth factor-beta family members are regulated during induced luteolysis in cattle

Variants in BMP7 and BMP15 3’-UTRs Associated with Reproductive Traits in a Large White Pig Population

Early growth response gene mediates in VEGF and FGF signaling as dissected by CRISPR in corpus luteum of water buffalo

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