Variants in BMP7 and BMP15 3’-UTRs Associated with Reproductive Traits in a Large White Pig Population

Yin, Hang; Du, Xiaohong; Pan, Zengxiang; Wu, Wangjun; Liu, Honglin

doi:10.3390/ani9110905

Cited by 10 publications

(5 citation statements)

References 47 publications

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“…Intronic mutations mostly affect mRNA shearing and folding at the molecular level and do not directly affect phenotypic traits (46, 47). For example, two intronic NOBOX mutations in Chinese women with premature ovarian failure (POF) were not associated with the disease (48), consistent with the intronic mutations in the present study, where g.1624 T>C, g.2770 G>A, and g.2821 A>G were not significantly associated with sow litter size traits (49,50). In the present study, the 3′-UTR mutation locus g.6025 T>A was significantly lower in the AA genotype, characterized by fewer litters and weaned piglets than in TT and TA genotypes in the second litter.…”

Section: Discussionsupporting

confidence: 82%

Association between polymorphisms in NOBOX and litter size traits in Xiangsu pigs

Huang,

Ruan,

Xiao

et al. 2024

Front. Vet. Sci.

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The newborn ovary homeobox gene (NOBOX) regulates ovarian and early oocyte development, and thus plays an essential role in reproduction. In this study, the mRNA expression level and single nucleotide polymorphism (SNP) of NOBOX in various tissues of Xiangsu pigs were studied to explore the relationship between its polymorphism and litter size traits. Also, bioinformatics was used to evaluate the effects of missense substitutions on protein structure and function. The results revealed that NOBOX is preferentially expressed in the ovary. Six mutations were detected in the NOBOX sequence, including g.1624 T>C, g.1858 G>A, g.2770 G>A, g.2821 A>G, g.5659 A>G, and g.6025 T>A, of which g.1858 G>A was a missense mutation. However, only g.1858 G>A, g.5659 A>G, and g.6025 T>A were significantly associated with litter size traits (p < 0.05). Further prediction of the effect of the missense mutation g.1858 G>A on protein function revealed that p.V82M is a non-conservative mutation that significantly reduces protein stability and thus alters protein function. Overall, these findings suggest that NOBOX polymorphism is closely related to the litter size of Xiangsu pigs, which may provide new insights into pig breeding.

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Section: Discussionsupporting

confidence: 82%

Association between polymorphisms in NOBOX and litter size traits in Xiangsu pigs

Huang,

Ruan,

Xiao

et al. 2024

Front. Vet. Sci.

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“…However, growth traits of Shaanbei White Cashmere goats need improving. Rapid, accurate, as well as an effective method of MAS is presented in [42][43][44]. Herein, this study aimed at finding an effective and faithful DNA maker which could accelerate the process of selective breeding in goat rearing.…”

Section: Introductionmentioning

confidence: 99%

Myostatin (MSTN) Gene Indel Variation and Its Associations with Body Traits in Shaanbei White Cashmere Goat

Feng

Wang

et al. 2020

Animals

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Myostatin (MSTN) gene, also known as growth differentiation factor 8 (GDF8), is a member of the transforming growth factor-beta super-family and plays a negative role in muscle development. It acts as key points during pre- and post-natal life of amniotes that ultimately determine the overall muscle mass of animals. There are several studies that concentrate on the effect of a 5 bp insertion/deletion (indel) within the 5’ untranslated region (5’ UTR) of goat MSTN gene in goats. However, almost all sample sizes were below 150 individuals. Only in Boer goats, the sample sizes reached 482. Hence, whether the 5 bp indel was still associated with the growth traits of goats in large sample sizes which were more reliable is not clear. To find an effective and dependable DNA marker for goat rearing, we first enlarged the sample sizes (n = 1074, Shaanbei White Cashmere goat) which would enhance the robustness of the analysis and did the association analyses between the 5 bp indel and growth traits. Results uncovered that the 5 bp indel was significantly related to body height, height at hip cross, and chest width index (p < 0.05). In addition, individuals with DD genotype had a superior growing performance than those with the ID genotype. These findings suggested that the 5 bp indel in MSTN gene are significantly associated with growth traits and the specific genotype might be promising for maker-assisted selection (MAS) of goats.

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“…The BMP7 gene is mainly enriched in several reproduction-related pathways and entries, such as embryonic placental development, urogenital development, and uterine embryo development. Previous studies have focused on it as a candidate gene related to reproductive traits, such as ovarian function, litter size, number of live-born piglets, and litter weight of piglets [70][71][72]. The QTL related to reproductive performance in BS pigs in this study were mainly concentrated on Chr1, Chr3, and Chr7, especially those related to ovary weight, litter size, and number of stillbirths, which are likely related to the regulatory pathway of MBP7.…”

Section: Discussionmentioning

confidence: 86%

Genome-Wide Detection for Runs of Homozygosity in Baoshan Pigs Using Whole Genome Resequencing

Li,

Wu,

Xiang

et al. 2024

Genes

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Baoshan pigs (BS) are a local breed in Yunnan Province that may face inbreeding owing to its limited population size. To accurately evaluate the inbreeding level of the BS pig population, we used whole-genome resequencing to identify runs of homozygosity (ROH) regions in BS pigs, calculated the inbreeding coefficient based on pedigree and ROH, and screened candidate genes with important economic traits from ROH islands. A total of 22,633,391 SNPS were obtained from the whole genome of BS pigs, and 201 ROHs were detected from 532,450 SNPS after quality control. The number of medium-length ROH (1–5 Mb) was the highest (98.43%), the number of long ROH (>5 Mb) was the lowest (1.57%), and the inbreeding of BS pigs mainly occurred in distant generations. The inbreeding coefficient FROH, calculated based on ROH, was 0.018 ± 0.016, and the FPED, calculated based on the pedigree, was 0.027 ± 0.028, which were positively correlated. Forty ROH islands were identified, containing 507 genes and 891 QTLs. Several genes were associated with growth and development (IGFALS, PTN, DLX5, DKK1, WNT2), meat quality traits (MC3R, ACSM3, ECI1, CD36, ROCK1, CACNA2D1), and reproductive traits (NPW, TSHR, BMP7). This study provides a reference for the protection and utilization of BS pigs.

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Variants in BMP7 and BMP15 3’-UTRs Associated with Reproductive Traits in a Large White Pig Population

Cited by 10 publications

References 47 publications

Association between polymorphisms in NOBOX and litter size traits in Xiangsu pigs

Association between polymorphisms in NOBOX and litter size traits in Xiangsu pigs

Myostatin (MSTN) Gene Indel Variation and Its Associations with Body Traits in Shaanbei White Cashmere Goat

Genome-Wide Detection for Runs of Homozygosity in Baoshan Pigs Using Whole Genome Resequencing

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