Expression and functional characterization of collection-K1 from Nile tilapia (Oreochromis niloticus) in host innate immune defense

Mu, Liangliang; Yin, Xiaoxue; Bian, Xia; Wu, Liting; Yang, Yanjian; Wei, Xin; Guo, Zheng; Ye, Jianmin

doi:10.1016/j.molimm.2018.08.012

Cited by 28 publications

(21 citation statements)

References 57 publications

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“…Our results show that OnGal8-L is up-regulated in a time-dependent manner and reached to peak at 12 h in spleen, head kidney, and brain after S. agalactiae infection, indicating that OnGal8-L plays a role in the anti-bacterial immune response of tilapia. Similar results are also observed in the studies of OnCL-K1 and OnGal-2 from Nile tilapia ( 17 , 27 ). It has been proven that S. agalactiae can invade the brain microvascular endothelial cell (BMEC) of fish, leading to meningitis ( 43 ).…”

Section: Discussionsupporting

confidence: 89%

“…Respiratory burst is crucial for degrading internalized particles and bacteria in phagocytes. It has been recorded that fish lectins, including C-type lectin ( 27 ), galectin ( 18 ), and rhamnose-binding lectin ( 51 ), can enhance the respiratory burst activity of monocyte/macrophages. Consistently, stronger respiratory burst of macrophage was detected after rOnGal8L treatment in this study, indicating a role of OnGal8-L as opsonin during pathogen invasion.…”

Section: Discussionmentioning

confidence: 99%

“…The bacterial growth inhibition assay of rOnGal8L was performed as described in a previous study (27). In brief, S. agalactiae and A. hydrophila were cultured overnight, the bacterial pellet was collected by centrifugation, and then adjusted to an initial concentration of 1 × 10 5 cells/mL with culture medium.…”

Section: Bacterial Growth Inhibition Assaymentioning

confidence: 99%

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Fish Galectin8-Like Exerts Positive Regulation on Immune Response Against Bacterial Infection

et al. 2020

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Galectin-8 is a member of the galectin family that is involved in immune response against pathogens. However, the roles of fish galectin-8 during pathogen infection require comprehensive studies. In this study, a galectin-8 homolog (OnGal8-like, OnGal8-L) was characterized from Nile tilapia (Oreochromis niloticus), and its roles in response to bacterial infection were analyzed. The OnGal8-L contains an open reading frame of 891 bp, encoding a peptide of 296 amino acids with two CRD regions of tandem-repeat galectin and two carbohydrate recognition sites. The OnGal8-L protein shares 46.42% identities with reported Oreochromis niloticus galectin-8 protein. Transcriptional expression analysis revealed that OnGal8-L was constitutively expressed in all examined tissues and was highly expressed in spleen. The transcript levels of OnGal8-L were up-regulated in the spleen, head kidney, and brain, following Streptococcus agalactiae (S. agalactiae) challenge. Further in vitro analysis indicated that the recombinant protein of OnGal8-L (rOnGal8L) could agglutinate erythrocyte, S. agalactiae, and A. hydrophila and bind S. agalactiae, A. hydrophila, and various PAMPs (lipopolysaccharides, lipoteichoic acid, poly I:C, peptidoglycan, galactose, mannose, and maltose). Also, rOnGal8L could regulate inflammatory-related gene expression, phagocytosis, and a respiratory burst of monocytes/macrophages. Moreover, in vivo analysis showed that OnGal8-L overexpression could protect O. niloticus from S. agalactiae infection through modulating serum antibacterial activity (AKP, ACP, and LZM), antioxidant capacity (CAT, POD, and SOD), and monocyte/macrophage proliferation and cytokine expression, as well as reducing bacterial burden and decreasing tissue damage. Our results collectively indicate that OnGal8-L plays important regulatory roles in immune response against bacterial infection.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Fish Galectin8-Like Exerts Positive Regulation on Immune Response Against Bacterial Infection

et al. 2020

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“…As we know, MO/MΦ are important effector cells that eliminate pathogenic microorganisms, which respond quickly to invading microorganisms and exert a pivotal role in non-specific cellular defense. The non-specific cellular immune defense is an important component of the fish innate defense system, including inflammation, migration, phagocytosis, and killing ( 37 , 48 ). OnMAP34 could not only involve the regulation of inflammation and migration reaction but also enhance MO/MΦ-mediated phagocytosis, thereby playing a regulatory role in host non-specific cellular defense.…”

Section: Discussionmentioning

confidence: 99%

“…To explore the effect of (r)OnMAp34 on phagocytosis, flow cytometer (FCM) analysis was implemented as previous descriptions ( 33 , 37 ). Briefly, the MO/MΦ (1 × 10 6 cells/mL) phagocytosing the FITC-labeled S. agalactiae and A. hydrophila (1 × 10 7 CFU/mL) were analyzed by FCM (BD, USA), in which the bacteria were pre-incubated with 100 μL of TBS, Trx (100 μg/mL), or (r)OnMAp34 (100 μg/mL) for 1 h. The non-ingested bacteria were removed, and the cell suspension was centrifuged over a cushion of 3% BSA in TBS supplemented with 4.5% D-glucose at 100 × g at 4°C for 10 min, repeated three times; then the pellets were re-suspended with TBS, and 1 μL of ice-cold trypan blue (0.4%) was added per sample ( 20 ).…”

Section: Methodsmentioning

confidence: 99%

MAp34 Regulates the Non-specific Cell Immunity of Monocytes/Macrophages and Inhibits the Lectin Pathway of Complement Activation in a Teleost Fish

Yin

et al. 2020

Front. Immunol.

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The lectin pathway of the complement system is one of the main components of innate immunity, which plays a pivotal role in the defense against infectious microorganisms and maintains immune homeostasis. However, its control mechanisms remain unclear in teleost fish. In this study, we described the identification and functional characterization of a mannose-binding lectin associated protein MAp34 (OnMAp34) from Nile tilapia ( Oreochromis niloticus ) at molecular, cellular, and protein levels. The open reading frame (ORF) of OnMAp34 is 918 bp of nucleotide sequence encoding a polypeptide of 305 amino acids. The deduced amino acid sequence has three characteristic structures, including two C1r/C1s-Uegf-BMP domains (CUB) and one epidermal growth factor domain (EGF). Expression analysis revealed that the OnMAp34 was highly expressed in the liver and widely existed in other examined tissues. In addition, the mRNA and protein expression levels of OnMAp34 were remarkably altered upon infection with Streptococcus agalactiae and Aeromonas hydrophila in vivo and in vitro . Further, we found that the OnMAp34 could participate in the non-specific cellular immune defense, including the regulation of inflammation, migration, and enhancement of phagocytosis of monocytes/macrophages. Moreover, the OnMAp34 could compete with OnMASPs to combine OnMBL and inhibit the lectin pathway of complement activation. Overall, our results provide new insights into the understanding of MAp34 as a potent regulator in the lectin complement pathway and non-specific cell immunity in an early vertebrate.

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Galectin-related protein from Oreochromis niloticus: Molecular, and transcriptional features and biological responses against microbial pathogens

Luo

Niu

Fang

et al. 2021

Aquaculture Reports

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Expression and functional characterization of collection-K1 from Nile tilapia (Oreochromis niloticus) in host innate immune defense

Cited by 28 publications

References 57 publications

Fish Galectin8-Like Exerts Positive Regulation on Immune Response Against Bacterial Infection

Fish Galectin8-Like Exerts Positive Regulation on Immune Response Against Bacterial Infection

MAp34 Regulates the Non-specific Cell Immunity of Monocytes/Macrophages and Inhibits the Lectin Pathway of Complement Activation in a Teleost Fish

Galectin-related protein from Oreochromis niloticus: Molecular, and transcriptional features and biological responses against microbial pathogens

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