Expression and clinical significance of C14orf166 in esophageal squamous cell carcinoma

Zhou, Yanwu; Li, Rong; Duan, Chaojun; Gao, Yang; Cheng, Yuanda; He, Zhiwei; Zeng, Jianping; Zhang, Chunfang

doi:10.3892/mmr.2016.6056

Cited by 6 publications

(5 citation statements)

References 38 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, we found that C14orf166 was constantly up-regulated in NSCLC cell lines. These results agree with our previous study of C14orf166 in esophagus squamous cell carcinoma [ 18 ], as well as in other previous studies [ 22 , 26 ].…”

Section: Discussionsupporting

confidence: 94%

“…In esophagus carcinoma cells, overexpressed C14orf166 has been reported to activate the abnormal JAK2/STAT3 signaling pathway by acting as a JH2-interacting protein, which induces excessive function, initiating carcinogenesis [ 15–17 ]. Additionally, along with our previous research in esophagus squamous cell carcinoma [ 18 ], C14orf166 is found to be overexpressed in several tumor tissues [ 19–22 ]; however, the role of C14orf166 in NSCLC is unknown, and this present study aims to clarify this potency.…”

Section: Introductionmentioning

confidence: 76%

See 1 more Smart Citation

Overexpressed C14orf166 associates with disease progression and poor prognosis in non-small-cell lung cancer

Zhou

Duan

et al. 2018

Bioscience Reports

Self Cite

View full text Add to dashboard Cite

Chromosome 14 ORF 166 (C14orf166), a protein involved in the regulation of RNA transcription and translation, has been reported to possess the potency to promote tumorigenesis; however, the role of C14orf166 in non-small-cell lung cancer (NSCLC) remains unknown. The purpose of the present study was to assess C14orf166 expression and its clinical significance in NSCLC. Immunohistochemical staining, quantitative real-time PCR (qRT-PCR), and Western blotting were used to detect the C14orf166 protein and mRNA expression levels in NSCLC tissues compared with adjacent normal tissues, as well as in NSCLC cells lines compared with normal human bronchial epithelial cells (HBE). Then, the correlations between the C14orf166 expression levels and the clinicopathological features of NSCLC were analyzed. Additionally, the Cox proportional hazard model was used to evaluate the prognostic significance of C14orf166. We found that C14orf166 expression increased in carcinoma tissues compared with their adjacent normal tissues at the protein (P<0.001) and mRNA levels (P<0.001). High expression of C14orf166 was significantly associated with the T stage (P=0.006), lymph node metastasis (P=0.001), advanced TNM stage (P<0.001), and chemotherapy (P<0.001). Moreover, according to the survival analysis, patients with overexpressed C14orf166 were inclined to experience a shorter overall survival and disease-free survival time (P<0.001). Multivariate COX analysis implied that C14orf166 was an independent prognostic biomarker. Taken together, our findings indicate that the overexpression of C14orf166 may contribute to the disease progression of NSCLC, represent a novel prognostic predictor and help high-risk patients make better decisions for subsequent therapy.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 76%

Overexpressed C14orf166 associates with disease progression and poor prognosis in non-small-cell lung cancer

Zhou

Duan

et al. 2018

Bioscience Reports

Self Cite

View full text Add to dashboard Cite

show abstract

“…Overexpression and activation of this receptor are commonly detected in colorectal cancer [15]. MEK1 can increase cell proliferation through EGF-EGFR-Ras-MEK-MAPK cascade [16]. In concordance with our results of MEK1 expression in ACS, Williet and colleagues [17] have also demonstrated that there is an increasing EGFR expression in the process of colorectal carcinogenesis, from adenomas showing low grade dysplasia (10% overexpression), and high grade dysplasia (77.8%), to carcinoma (100%).…”

Section: Discussionmentioning

confidence: 99%

Role of MEK1 and DIAPH3 expression in colorectal adenoma-carcinoma sequence

Foda,

El-Hawary,

Elnaghi

et al. 2024

TUB

View full text Add to dashboard Cite

BACKGROUND: It is well established that most colorectal carcinomas arise from conventional adenomas through the adenoma-carcinoma sequence (ACS) model. mitogen-activated protein kinases (MAPKs) pathway has been reported as a crucial player in tumorigenesis. The MAPK signaling pathway is activated by different extracellular signals involving the “mitogen-activated/extracellular signal-regulated kinase 1 (MEK1)”, and this induces the expression of genes involved in proliferation and cellular transformation. Diaphanous-related formin-3 (DIAPH3) acts as a potential metastasis regulator through inhibiting the cellular transition to amoeboid behavior in different cancer types. OBJECTIVE: The aim of the study was to investigate the pattern of immunohistochemical expression of MEK1 and DIAPH3 in colorectal adenoma (CRA) and corresponding colorectal carcinoma (CRC) specimens. METHODS: The immunohistochemical expression of DIAPH3 and MEK1 was examined in 43 cases of CRC and their associated adenomas using tissue microarray technique. RESULTS: MEK1 was overexpressed in 23 CRC cases (53.5%) and in 20 CRA cases (46.5%). DIAPH3 was overexpressed in 11 CRA cases (about 29%) which were significantly lower than CRC (22 cases; 58%) (P = 0.011). Both MEK1 and DIAPH3 overexpression were significantly correlated in CRC (P = 0.009) and CRA cases (P = 0.002). Tumors with MEK1 overexpression had a significantly higher tumor grade (P = 0.050) and perineural invasion (P = 0.017). CONCLUSIONS: Both MEK1 and DIAPH3 are overexpressed across colorectal ACS with strong correlation between them. This co- expression suggests a possible synergistic effect of MEK1 and DIAPH-3 in colorectal ACS. Further large-scale studies are required to investigate the potential functional aspects of MEK1 and DIAPH3 in ACS and their involvement in tumor initiation and the metastatic process.

show abstract

“…Additionally, the RTRAF gene overexpression in human nasopharyngeal and cervical carcinoma cells and tissues has been linked to suboptimal patient outcomes, larger tumor sizes, metastasis to lymph nodes, decreased survival rates, and early patient mortality (23,24). Moreover, following the stabilization of RTRAF gene expression, progression and metastasis to lymph nodes were observed in both esophageal and pancreatic cancers (25,26). In both low-grade and high-grade brain neoplasms, the RTRAF gene expression does not exhibit a noteworthy disparity, proposing that the RTRAF gene overexpression is of paramount importance in the genesis of tumors during the initial phases of tumorigenesis (14).…”

Section: Discussionmentioning

confidence: 99%

The Act of Diminishing the RTRAF Expression in Benign Thyroid Tissues Represents a Potential Method for Impeding the Progression of Malignancy in Cells

Esfandiarpoor,

Javadirad,

Kolahduzan

2023

Jentashapir J Cell Mol Biol

View full text Add to dashboard Cite

Background: Thyroid cancer is the most common endocrine cancer, and women are almost three times more likely to develop it than men. Objectives: The etiology of thyroid cancer at the genetic level remains elusive. However, a potential involvement of the RNA Transcription, Translation, and Transport Factor (RTRAF) gene in the pathogenesis of this malignancy has been postulated. This gene has been shown to govern the expression of proteins that exert regulatory effects on the transition from the G1 to S phase of the cell cycle. Methods: For this study, 22 papillary thyroid carcinoma (PTC) tissues and 22 healthy tissues were collected. Additionally, 10 benign goiter tissues and 10 healthy tissues were gathered. RNA molecules were fixed, and complementary DNA (cDNA) was produced. Specific primers were used to measure the RTRAF gene expression. Statistical analysis was conducted using REST 2009 software. Results: Upon assessing the quality and quantity of RNA, it was ascertained that the extracted RNA molecules exhibited minimal damage and were found to be appropriately concentrated for cDNA production. The amplification of the RTRAF gene was carried out accurately, as evidenced by the melting curve. A noteworthy decline in the RTRAF gene expression was detected in the benign goiter tissue compared to the adjacent healthy tissue, with a relative expression of 0.154 and a statistical value of P = 0.002. Conversely, there was no significant difference in the RTRAF gene expression between PTC and the adjacent healthy tissue, with a P-value of 0.808. Conclusions: The reduced RTRAF gene expression in benign thyroid tumors may hinder cancer cell growth, as no difference was observed between malignant PTC tumor tissues and their healthy tissues.

show abstract

Expression and clinical significance of C14orf166 in esophageal squamous cell carcinoma

Cited by 6 publications

References 38 publications

Overexpressed C14orf166 associates with disease progression and poor prognosis in non-small-cell lung cancer

Overexpressed C14orf166 associates with disease progression and poor prognosis in non-small-cell lung cancer

Role of MEK1 and DIAPH3 expression in colorectal adenoma-carcinoma sequence

The Act of Diminishing the RTRAF Expression in Benign Thyroid Tissues Represents a Potential Method for Impeding the Progression of Malignancy in Cells

Contact Info

Product

Resources

About