Overexpressed C14orf166 associates with disease progression and poor prognosis in non-small-cell lung cancer

Abstract

Chromosome 14 ORF 166 (C14orf166), a protein involved in the regulation of RNA transcription and translation, has been reported to possess the potency to promote tumorigenesis; however, the role of C14orf166 in non-small-cell lung cancer (NSCLC) remains unknown. The purpose of the present study was to assess C14orf166 expression and its clinical significance in NSCLC. Immunohistochemical staining, quantitative real-time PCR (qRT-PCR), and Western blotting were used to detect the C14orf166 protein and mRNA expression levels in NSCLC tissues compared with adjacent normal tissues, as well as in NSCLC cells lines compared with normal human bronchial epithelial cells (HBE). Then, the correlations between the C14orf166 expression levels and the clinicopathological features of NSCLC were analyzed. Additionally, the Cox proportional hazard model was used to evaluate the prognostic significance of C14orf166. We found that C14orf166 expression increased in carcinoma tissues compared with their adjacent normal tissues at the protein (P<0.001) and mRNA levels (P<0.001). High expression of C14orf166 was significantly associated with the T stage (P=0.006), lymph node metastasis (P=0.001), advanced TNM stage (P<0.001), and chemotherapy (P<0.001). Moreover, according to the survival analysis, patients with overexpressed C14orf166 were inclined to experience a shorter overall survival and disease-free survival time (P<0.001). Multivariate COX analysis implied that C14orf166 was an independent prognostic biomarker. Taken together, our findings indicate that the overexpression of C14orf166 may contribute to the disease progression of NSCLC, represent a novel prognostic predictor and help high-risk patients make better decisions for subsequent therapy.

An in silico prediction of interaction models of influenza A virus PA and human C14orf166 protein from yeast‐two‐hybrid screening data

“…It has been suggested that the overexpression of C14orf166 has a role in the development of nonsmall-cell lung cancer, and it would be beneficial to consider it in the treatment of high-risk patients. 33 It has been reported that this protein is overexpressed in breast cancer. 34 The expression of both the C14orf166 mRNA and protein was also found to be upregulated in cervical cancer cell lines and tissues.…”

“…The C14orf166, a multifunctional protein with these properties, is associated with some cancer types. It has been suggested that the overexpression of C14orf166 has a role in the development of nonsmall‐cell lung cancer, and it would be beneficial to consider it in the treatment of high‐risk patients 33 . It has been reported that this protein is overexpressed in breast cancer 34 .…”

An in silico prediction of interaction models of influenza a virus PA and human C14orf166 protein from yeast-two-hybrid screening data

“…The C14orf166, a multifunctional protein with these properties, is associated with some cancer types. It has been suggested that the overexpression of C14orf166 has a role in the development of non-small-cell lung cancer (NSCLC), and it would be beneficial to consider it in the treatment of high-risk patients 32 . It has been reported that this protein is overexpressed in breast cancer 33 .…”

Identification of Functional Interactome of Gastric Cancer Cells with Helicobacter pylori Outer Membrane Protein HpaA by HPLC-MS/MS

“…PRDX1 could modulate cellular apoptosis via scavenging intracellular reactive oxygen species in AGS cells [44,45]. IRF2BP2 and C14orf166 have been reported to possess the potency to promote tumorigenesis [46,47]. IRF2BP2 as a transcriptional repressor overexpresses in gastric cancer cells, which is closely related to proliferation, migration, and invasion in gastric cancer [48].…”