Expression and characterization of the benzoic acid hydroxylase CYP199A25 from Arthrobacter sp.

Genomic insight into O-demethylation of 4-methoxybenzoate by a two-component system from Amycolatopsis magusensis KCCM40447

“…The function prediction of the 4-methoxybenzoate monoxygenase was further analyzed by phylogenesis and multiple sequences alignment with other homologous enzymes known for the O-demethylation of aromatic compounds [ [10] , [11] , [12] , [13] ]. The higher identity and conservation at the protein sequence level represent this monoxygenase may be responsible for the O -demethylation of different benzoic acid derivatives.…”

“…In addition, CYP199A25 from Arthrobacter sp. was responsible for hydroxylation or demethylation only in the para position using the non-physiological redox partners putidaredoxin reductase (Pdr) and putidaredoxin (Pdx) (class I CYP101A1 system of Pseudomonas putida) [ 13 ].…”

Cytochromes P450 in the biocatalytic valorization of lignin

“…The two best characterized CYP199As, CYP199A2 from Rhodopseudomonas palustris CGA009 [31] and CYP199A4 from R. palustris HaA2 [32], have strongest preference for 4-methoxybenzate as substrate, but also turnover veratrate efficiently and selectively oxidize the 4-substituent of chemically diverse benzoates [33][34][35]. CYP199A25, identified in a papaverine-degrading Arthrobacter sp., also catalyzes the O-demethylation of 4-methoxybenzoate and has high affinity for veratrate [36], while CYP199A3 is predicted to be responsible for growth on 4-methoxybenzoate in RHA1 (Wolf and Eltis, unpublished). CYP199A2 and CYP199A4 are part of three-component systems, with a ferredoxin and a two-domain reductase (Figure 2) [32].…”

A High-Efficiency Artificial Synthetic Pathway for 5-Aminovalerate Production From Biobased L-Lysine in Escherichia coli

“…Increasing concerns over global water pollution, climate change, public health, and petroleum shortages have attracted considerable attention to sustainable development as promising green alternatives to traditional petrochemical-derived chemicals renewable feedstock ( Tsuge et al, 2016 ). Recently a variety of valuable chemicals such as 6-aminocaproate ( Cheng et al, 2019 ), fructose ( Yang et al, 2016 ), mandelic acid ( Youn et al, 2020 ), vitamin B 12 ( Fang et al, 2018 ), naringenin ( Gao et al, 2020b ), p -coumaric acid ( Gao et al, 2020a ), breviscapine ( Liu et al, 2018 ), 4-hydroxybenzoic acid ( Klenk et al, 2020 ), curcuminoids ( Rodrigues et al, 2020 ) and hydroxytyrosol ( Zeng et al, 2020 ) have been produced in microorganisms. As a kind of green alternative to petrochemical products, microbial bioplastics are composed of monomers containing appropriate functional groups, which have become the focus of metabolic engineering research.…”