Expression and biochemical characterization and substrate specificity of the fucoidanase from<i>Formosa algae</i>

Silchenko, Artem S.; Ustyuzhanina, Nadezhda E.; Kusaykin, Mikhail I.; Krylov, Vadim B.; Shashkov, Alexander S.; Dmitrenok, Andrey S.; Usoltseva, Roza V.; Zueva, Anastasiya O.; Nifantiev, Nikolay E.; Zvyagintseva, T. N.

doi:10.1093/glycob/cww138

Cited by 30 publications

(82 citation statements)

References 35 publications

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“…This indicated a hydrolytic activity against a-1,4-linked fucan, since fucans extracted from Laminariales are known to be composed of an a-1,3-linked fucose backbone [2,52]. This is also in agreement with the activities previously observed for the characterized GH107 counterparts, FcnA from M. fucanivorans and FFA2 from F. algae, which were both found to cleave a-1,4-linked fucans [26,27]. Based on the distinct C-PAGE profiles obtained for Fucus serratus, F. vesiculosus, and Fucus spiralis when hydrolyzed with the same fucanase, sulfated fucans seem to be highly structurally diverse, even between closely related algal species.…”

Section: Novel Gh107s Exclusively Degrade Sulfated Fucans Derived Frosupporting

confidence: 90%

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Discovery and screening of novel metagenome‐derived GH107 enzymes targeting sulfated fucans from brown algae

et al. 2018

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Sulfated fucans, often denoted as fucoidans, are highly variable cell wall polysaccharides of brown algae, which possess a wide range of bioactive properties with potential pharmaceutical applications. Due to their complex architecture, the structures of algal fucans have until now only been partly determined. Enzymes capable of hydrolyzing sulfated fucans may allow specific release of defined bioactive oligosaccharides and may serve as a tool for structural elucidation of algal walls. Currently, such enzymes include only a few hydrolases belonging to the glycoside hydrolase family 107 (GH107), and little is known about their mechanistics and the substrates they degrade. In this study, we report the identification and recombinant production of three novel GH107 family proteins derived from a marine metagenome. Activity screening against a large substrate collection showed that all three enzymes degraded sulfated fucans from brown algae in the order Fucales. This is in accordance with a hydrolytic activity against α-1,4-fucosidic linkages in sulfated fucans as reported for previous GH107 members. Also, the activity screening gave new indications about the structural differences in brown algal cell walls. Finally, sequence analyses allowed identification of the proposed catalytic residues of the GH107 family. The findings presented here form a new basis for understanding the GH107 family of enzymes and investigating the complex sulfated fucans from brown algae. DATABASE: The assembled metagenome and raw sequence data is available at EMBL-EBI (Study number: PRJEB28480). Sequences of the GH107 fucanases (Fp273, Fp277, and Fp279) have been deposited in GenBank under accessions MH755451-MH755453.

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Section: Novel Gh107s Exclusively Degrade Sulfated Fucans Derived Frosupporting

confidence: 90%

“…These observations give new indications that members of the GH107 family display individual substrate preference for certain algal fucan structures. This may be due to a difference in sulfation, which has previously been found to influence fucanase activity . Yet, additional work is required for confirmation.…”

Section: Resultsmentioning

confidence: 95%

Discovery and screening of novel metagenome‐derived GH107 enzymes targeting sulfated fucans from brown algae

et al. 2018

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“…Despite the increasing number of publications investigating fucoidanase activity of different marine species cell extracts, few of these enzymes have been isolated and characterized. Moreover, genome sequences encoding few fucoidanases have been published, including Ffa2 and FFA1 from Formosa algae KMM 3553 T [182,183], FcnA from Mariniflexili fucanivorans SW5T [184]. Therefore, specificity of fucoidanases, type of cleaved glycoside bond, structure-activity relationship studies and enzyme stability are still poorly described.…”

Section: Enzymatic Modification Of Native Fucoidansmentioning

confidence: 99%

“…Similarly, recent advances in bioinformatics and genome sequencing of microbial species have resulted in a continual increase of novel genome sequences. These genomes demonstrated various potential genes encoding for enzymes with biopolymer-degrading capabilities, such as Shewanella violacea DSS12 (NC_014012.1), Formosa algae KMM 3553 (NZ_LMAK01000014.1) [182], Formosa haliotis MA1 (NZ_BDEL01000001.1) [198], Wenyingzhuangia fucanilytica CZ1127 (NZ_CP014224.1) [199] and Pseudoalteromonas sp. strain A601 (MXQF01000000) [200].…”

Section: Enzymatic Modification Of Native Fucoidansmentioning

confidence: 99%

Fucoidans: Downstream Processes and Recent Applications

Zayed

Ulber

2020

Marine Drugs

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Fucoidans are multifunctional marine macromolecules that are subjected to numerous and various downstream processes during their production. These processes were considered the most important abiotic factors affecting fucoidan chemical skeletons, quality, physicochemical properties, biological properties and industrial applications. Since a universal protocol for fucoidans production has not been established yet, all the currently used processes were presented and justified. The current article complements our previous articles in the fucoidans field, provides an updated overview regarding the different downstream processes, including pre-treatment, extraction, purification and enzymatic modification processes, and shows the recent non-traditional applications of fucoidans in relation to their characters. of 22reproductive, immune and cell-to-cell communicative roles [23]. As recommended by the International Union of Pure and Applied Chemistry (IUPAC), fucoidans is a general term used to describe sulfated L-fucose-based polymers including sulfated fucans cited by the Swedish scholar Kylin, as well as other fucose-rich sulfated heteropolysaccharides [23,28]. Their chemical structures, in terms of monomeric composition and branching, are quite simple in marine invertebrates compared to their analogues in brown algae [13,29].Hundreds of articles have thoroughly discussed and reviewed the biological, pharmacological and pharmaceutical applications of fucoidans [30][31][32][33], including nanomedicine, [34] which has made it a hot topic in the last few decades [35][36][37]. All these studies tried to investigate fucoidans molecular mechanisms in relation to their chemical structure and physicochemical properties. Therefore, different hypotheses were suggested for each activity, such as anti-tumor [31,[38][39][40], anti-coagulant [41,42], anti-viral [43,44] and anti-inflammatory activity [45,46]. These investigations revealed that various factors are relevant, such as molecular weight, sulfation pattern, sulfate content and monomeric composition [47][48][49]. For example, different fractions were produced with different physicochemical properties in our previous experiments; sulfation pattern and sulfate content were highly related to anti-viral and cytotoxic activities against HSV-1 and Caco-2 cell lines, respectively, while molecular weight and sugar composition were potential factors in anti-coagulation activity [41,50]. In addition, degree of purity was reported as an influential factor [32], where co-extracted contaminants (e.g., phlorotannins or polyphenols) could lead to significant interference in anti-oxidant activity and, consequently, cosmetic applications [51,52].Therefore, several key production challenges regarding fucoidans were discussed in our last review article in order to obtain a product that follows the universal good manufactured practice (GMP) guidelines. The article discussed sources of heterogeneity in extracted fucoidans, including the different biotic (e.g., biogenic, geographical and seas...

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“…So, as affected of alkaline fucoidanаse from Littorina kurila yield of LMW sulfated products from F. evanescens which was three times higher compare with action of acid fucoidanase was observed [143,144]. By means of enzymatic hydrolysis of fucoidan from brown alga F. evanescens using of fucoidanase from marine bacteria Formosa algae the product with Mm ~9 kDa was obtained [141,145]. From the results of nuclear magnetic resonance (NMR)-analysis, this fraction represents regular PS, with the following structure of repeating unit:…”

Section: Oligosaccharidesmentioning

confidence: 99%

The Sulfated Polysaccharides of Brown Algae and Products of Their Enzymatic Transformation as Potential Vaccine Adjuvants

Кузнецова

Persiyanova²,

Ermakova

et al. 2018

Natural Product Communications

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The review is devoted to critical analysis of literature data, deal with effects and mechanisms of action of sulfated polysaccharides (PSs)-fucoidans from brown algae and products of their enzymatic transformation as potential adjuvants for enhancement of anti-infective and antitumor immune response. Numerous experimental data indicate that sulfated PSs demonstrate properties of vaccine adjuvants. Application perspectiveness of fucoidans as vaccine adjuvants is defined by their high biocompatibility, low-toxicity, safety and good tolerance by macroorganism, and also mechanisms of their immunomodulatory action. In particular, fucoidans are agonists of receptors of innate immunity and strong inducers of cellular and humoral immune response. At presenting the data of structural-functional interrelations, attention focused to the defining role of degree of sulfation, uronic acids and polyphenols contents, and also molecular mass in actions of fucoidans to innate and adaptive immunity cells. Insufficiency of literary data on studying of correlation of structure-physicochemical characteristics with adjuvanticities of the sulfated PSs, and also the problem of standardization of their active fractions are noted. Special attention is paid to the analysis of immunomodulatory and adjuvant activity of fucoidan oligosaccharides. Presented here results of experimental trial indicate that, despite the difficulties due to preparation of highly purified structurally characterized fractions and complex structure of fucoidans, these substances can be used as safe and effective adjuvants in vaccines against various pathogens including viruses, and also in antitumor vaccines.

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Expression and biochemical characterization and substrate specificity of the fucoidanase fromFormosa algae

Cited by 30 publications

References 35 publications

Discovery and screening of novel metagenome‐derived GH107 enzymes targeting sulfated fucans from brown algae

Discovery and screening of novel metagenome‐derived GH107 enzymes targeting sulfated fucans from brown algae

Fucoidans: Downstream Processes and Recent Applications

The Sulfated Polysaccharides of Brown Algae and Products of Their Enzymatic Transformation as Potential Vaccine Adjuvants

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