Expression and antiviral activity of a β-defensin-like peptide identified in the rainbow trout (Oncorhynchus mykiss) EST sequences

Falcó, Alberto; Chico, V.; Marroquí, Laura; Pérez, Luis; Coll, Julio; Estepa, A.

doi:10.1016/j.molimm.2007.06.358

Cited by 112 publications

(83 citation statements)

References 44 publications

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“…The effect of trout rIL-17A/F on the expression of antimicrobial peptides (AMPs) was studied with a particular focus on the ␤-defensins, since they are known to be modulated by T H 17 cytokines in mammals (10,33,35,72). ␤-defensins have been well characterized in previous rainbow trout reports (37,73). In this study, ␤-defensin 3 (BD-3) was selected as a marker gene, since previous work (36) demonstrated a clear effect of fish recombinant IL-22 on BD-3 expression.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of Rainbow Trout Interleukin-17A/F2 (IL-17A/F2) and IL-17 Receptor A: Expression during Infection and Bioactivity of Recombinant IL-17A/F2

et al. 2013

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Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of Rainbow Trout Interleukin-17A/F2 (IL-17A/F2) and IL-17 Receptor A: Expression during Infection and Bioactivity of Recombinant IL-17A/F2

et al. 2013

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“…Zhou et al [179] used EST and complete genome data to identify defensins from zebrafish (Dario rerio) and pufferfish (Takifugu rubripes) that resemble the β-defensins of birds and mammals. Falco et al [182], using a recombinant protein based on rainbow trout defensin, found it to be antiviral against viral haemorrhagic septicaemia rhabdovirus (VHSV), one of the most troublesome diseases in fish aquaculture. More recent studies have cloned three novel β-defensins from rainbow trout, all of which appear to be constitutively expressed but increase in expression during bacterial and simulated viral challenges [180].…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

An Overview of the Immunological Defenses in Fish Skin

2012

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The vertebrate immune system is comprised of numerous distinct and interdependent components. Every component has its own inherent protective value, and the final combination of them is likely to be related to an animal's immunological history and evolutionary development. Vertebrate immune system consists of both systemic and mucosal immune compartments, but it is the mucosal immune system which protects the body from the first encounter of pathogens. According to anatomical location, the mucosa-associated lymphoid tissue, in teleost fish is subdivided into gut-, skin-, and gill-associated lymphoid tissue and most available studies focus on gut. The purpose of this paper is to summarise the current knowledge of the immunological defences present in skin mucosa as a very important part of the fish immune system, serving as an anatomical and physiological barrier against external hazards. Interest in defence mechanism of fish arises from a need to develop health management tools to support a growing finfish aquaculture industry, while at the same time addressing questions concerning origins and evolution of immunity in vertebrates. Increased knowledge of fish mucosal immune system will facilitate the development of novel vaccination strategies in fish.

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“…They are expressed in pre-peptidic form by many cell types (keratinocytes, macrophages, neutrophils, lymphocytes), and stored in vesicles that are released upon infection [56]. Multiple β-defensin genes have been identified in puffer fish (Takifugu rubripes), spotted puffer fish (Tetraodon nigroviridis), zebrafish (Danio rerio), and trout (Oncorhynchus mykiss) [16,57]. The β-defensin gene is constitutively expressed in all below species during adulthood, and their expression and localization includes gills, head kidney, liver, intestine, gonads, and spleen.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Peptides Are Expressed during Early Development of Zebrafish (Danio rerio) and Are Inducible by Immune Challenge

Caccia

Agnello

Ceci

et al. 2017

Fishes

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Antimicrobial peptides (AMPS) are ancestral components in the evolution of immunity from protozoans to metazoans. Their expression can be constitutive or inducible by infectious challenge. Although characterized in detail in their structure and activity, the temporal and spatial expression of AMPS during vertebrate embryogenesis is still poorly understood. In the present study, we identified selected AMPs in zebrafish, and characterized their expression during early development, and upon experimental immune challenge in adult animals, with the goal of establishing this genetically-tractable model system for further AMP studies. By mining available genomic databases, zebrafish AMP sequences homologous to AMPs from other vertebrates were selected for further study. These included parasin I and its enzyme cathepsin D, β-defensin (DB1), liver-expressed antimicrobial peptide 2 (LEAP2), bactericidal permeability-increasing protein (BPI), and chromogranin-A and -B (CgA and CgB). Specific primers were designed for RT-PCR amplification of each AMP gene of interest and amplicons between 242 bp and 504 bp were obtained from RNA extracted from adult zebrafish. Sequencing of the amplicons and alignment of their deduced amino acid sequences with those from AMPs from other vertebrate species confirmed their identity. The temporal expression of AMPs was investigated by RT-PCR analysis in fertilized oocytes, embryos, and adult individuals. Parasin I and chatepsin D transcripts were detectable immediately after fertilization, while the transcripts for CgA and CgB became evident starting at 48 h post fertilization. Mature transcripts of LEAP2 and DB1 were detectable only in the adult zebrafish, while BPI transcripts were detectable starting from the 12th day post fertilization. To explore the possible upregulation of AMP expression by infectious challenge, experiments were carried out in adult zebrafish by intraperitoneal injection of a cocktail of lipopolysaccharide and lipoteichoic acid. Except for CgA and CgB, amplicons corresponding to all tested AMPs showed stronger signals in the experimental animals as compared to the unchallenged controls. This study provided information on the early expression of AMPs in zebrafish from ontogeny to adulthood and their inducibility by microbials. This information could be useful to actuate new prophylactic strategies as an alternative to the use of antibiotics in culture.

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Expression and antiviral activity of a β-defensin-like peptide identified in the rainbow trout (Oncorhynchus mykiss) EST sequences

Cited by 112 publications

References 44 publications

Cloning and Characterization of Rainbow Trout Interleukin-17A/F2 (IL-17A/F2) and IL-17 Receptor A: Expression during Infection and Bioactivity of Recombinant IL-17A/F2

Cloning and Characterization of Rainbow Trout Interleukin-17A/F2 (IL-17A/F2) and IL-17 Receptor A: Expression during Infection and Bioactivity of Recombinant IL-17A/F2

An Overview of the Immunological Defenses in Fish Skin

Antimicrobial Peptides Are Expressed during Early Development of Zebrafish (Danio rerio) and Are Inducible by Immune Challenge

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