Expression and activity of sulfate transporters and APS reductase in curly kale in response to sulfate deprivation and re-supply

Koralewska, A.; Büchner, Peter; Stuiver, C.E.E.; Posthumus, Freek; Kopriva, Stanislav; Hawkesford, Malcolm J.; Kok, Luit J. De

doi:10.1016/j.jplph.2008.03.005

Cited by 50 publications

(42 citation statements)

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“…Selenate supply had little effect on transcript abundance, and S starvation only resulted in a slight increase in the expression of group 1 and group 4 transporters in root tissues. This is in contrast to observations with other species, where the expression of many sulfate transporters has been shown to be highly induced by sulfate starvation (Smith et al, 1995(Smith et al, , 1997Takahashi et al, 2000;Shibagaki et al, 2002;Yoshimoto et al, 2002Yoshimoto et al, , 2003Howarth et al, 2003;Buchner et al, 2004aBuchner et al, , 2004bHopkins et al, 2004Hopkins et al, , 2005Koralewska et al, 2008Koralewska et al, , 2009Rouached et al, 2008;Shinmachi et al, 2010). The expression of one group 1 isoform, Sultr1;1, and one group 2 isoform, Sultr2;1, has also been shown to be induced by increased Se supply (Takahashi et al, 2000).…”

Section: Discussioncontrasting

confidence: 93%

A Comparison of Sulfate and Selenium Accumulation in Relation to the Expression of Sulfate Transporter Genes in Astragalus Species

et al. 2011

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Sulfate and selenate uptake were investigated in both selenium (Se) hyperaccumulators (Astragalus racemosus and Astragalus bisulcatus) and closely related nonaccumulator species (Astragalus glycyphyllos and Astragalus drummondii). Sulfur (S) starvation increased Se accumulation, whereas increased selenate supply increased sulfate accumulation in both root and shoot tissues. cDNAs for homologs of groups 1 to 4 sulfate transporters were cloned from these Astragalus species to investigate patterns of expression and interactions with sulfate and selenate uptake. In contrast to all other previously analyzed plant species, abundant gene expression of putative sulfate transporters was observed for both Se-hyperaccumulating and nonaccumulating Astragalus, regardless of S and Se status. Furthermore, quantitative analysis of expression indicated a transcript level in Sehyperaccumulating Astragalus comparable with other plant species under S deprivation. The high expression of sulfate transporters in certain Astragalus species may lead to enhanced Se uptake and translocation ability and therefore may contribute to the Se hyperaccumulation trait; however, it is not sufficient to explain S/Se discriminatory mechanisms. Selenium (Se) is an essential element for human and animal health, but excess consumption may also be toxic, and the concentration range from trace element requirement to lethality is narrow (Ohlendorf et al

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Section: Discussioncontrasting

confidence: 93%

A Comparison of Sulfate and Selenium Accumulation in Relation to the Expression of Sulfate Transporter Genes in Astragalus Species

et al. 2011

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“…Sultr1;3 is presumably involved in phloem loading of sulfate (Yoshimoto et al, 2003 ). The abundance and expression of the Group 1 sulfate transporters in roots is species specifi c. For instance, in roots of dicotyledons (e.g., Arabidopsis , Brassica ), Sultr1;2 was the sole constitutively expressed sulfate transporter present, whereas Sultr1;1 was only expressed upon sulfate deprivation (Buchner et al, 2004a,b ;Koralewska et al, 2007Koralewska et al, , 2008Koralewska et al, , 2009aParmar et al, 2007 ). In the monocotyledon wheat, however, the Sultr1;2 -type transporter appeared to be absent in the roots, and here the Sultr1; -type sulfate transporter was expressed .…”

Section: Sulfate Uptake Distribution and Assimilation In Plantsmentioning

confidence: 99%

“…For instance, in Brassica there was generally no clear relation between the overall tissue levels of sulfate, thiols, and O -acetylserine in the root or shoot and the expression and activity of the sulfate transporters upon variation in sulfate supply (Buchner et al, 2004a ;Koralewska et al, 2007Koralewska et al, , 2008Koralewska et al, , 2009aStuiver et al, 2009 ;Shahbaz et al, 2010 ). First, the fast upregulation of expression and activity of the sulfate transporters in Brassica upon sulfate deprivation usually already occurs without substantial changes in the overall tissue sulfate and/or thiol (viz.…”

Section: Regulation Of Uptake Distribution and Assimilation Of Sulfmentioning

confidence: 99%

“…Sulfate deprivation generally results in a rapid upregulation of the expression of the high -affi nity Group 1 sulfate transporters in the roots and, upon prolonged deprivation, also in the shoot (Buchner et al, 2004a(Buchner et al, ,b, 2010Koralewska et al, 2007Koralewska et al, , 2008Koralewska et al, , 2009aParmar et al, 2007 ;Stuiver et al, 2009 ;Shinmachi et al, 2010 ). Furthermore, the Group 2 (vascular transport) and Group 4 transporters (vacuolar effl ux) are highly upregulated in the root and that of Group 4 transporters also in the shoot (Parmar et al, 2007 ;Stuiver et al, 2009 ;Koralewska et al, 2007Koralewska et al, , 2008Koralewska et al, , 2009aBuchner et al, 2004aBuchner et al, , 2010Shinmachi et al, 2010 ). Apparently upon the occurrence of sulfur defi ciency plants try to remobilize and redistribute all possible available sulfate from, for instance, vacuoles and other resources.…”

Section: Regulation Of Uptake Distribution and Assimilation Of Sulfmentioning

confidence: 99%

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Sulfur Nutrition in Crop Plants

Kok

Stulen

Hawkesford

2011

The Molecular and Physiological Basis of Nutrient Use Efficiency in Crops

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Sulfur is an essential element for plant growth, and together with nitrogen it is necessary for the synthesis of amino acids, proteins, and various other cellular components, including thiol compounds and so -called secondary sulfur compounds, which play an important role in the protection of plants against stress and pests. Sulfur fertilization is often necessary to obtain optimal crop yield and quality. The timing and form of sulfur fertilizer is of great importance since the rate of remobilization of sulfur from older to young tissue can be low in some crops. Sulfate moving through the soil toward the surface of root by mass/bulk fl ow and taken up with high affi nity by the roots (apparent K m < 10 μ M), is the primary sulfur source for plants. The sulfate is reduced in the chloroplasts/plastids prior to its assimilation into organic sulfur compounds. The uptake of sulfate by the root is a primary controlling factor in plant sulfur nutrition and is adjusted to the sulfur demand for growth. The uptake and distribution of sulfate in the plants is mediated by distinct sulfate transporters. Both the expression and activity of the sulfate transporters and adenosine 5 ′ phosphosulfate (APS) reductase, the key enzyme of the sulfate reduction pathway, are modulated by the sulfur nutritional status. The signal transduction pathway in the regulation of the sulfate transporters is not well elucidated. It is uncertain to what extent the measurement of changes in concentrations of potential signal compounds, determined at the whole -plant organ level, provides suffi cient insight into the actual regulatory control of the sulfate uptake at the cellular level. Furthermore, a key unresolved issue is the signal transduction pathway in the crosstalk between the sulfate reduction pathway in the chloroplasts/plastids and the transcription of sulfate transporters/sulfate -reducing enzymes in the nucleus. With the recent appearance of sulfur defi ciency in agricultural systems and the awareness of the importance in quality and stress resistance, possibilities for targeting improvements in sulfur use effi ciency may be required and are discussed.The Molecular and Physiological Basis of Nutrient Use Effi ciency in Crops, First Edition. Edited by Malcolm J. Hawkesford, Peter Barraclough.

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“…For example, increasing expression levels were found under sulfur limitation when the sulfate uptake capacity increased (Smith et al 1997;Vidmar et al 2000;Buchner et al 2004b;Hopkins et al 2004;Kataoka et al 2004b;Rouached et al 2008;Koralewska et al 2009). Moreover, reduced or increased expression was also observed during heavy metal application (Heiss et al 1999;Nocito et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Seasonal and cell type specific expression of sulfate transporters in the phloem of Populus reveals tree specific characteristics for SO4 2− storage and mobilization

Dürr¹,

Bücking

Mult³

et al. 2010

Plant Mol Biol

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The storage and mobilization of nutrients in wood and bark tissues is a typical feature of trees. Sulfur can be stored as sulfate, which is transported from source to sink tissues through the phloem. In the present study two transcripts encoding sulfate transporters (SULTR) were identified in the phloem of grey poplar (Populus tremula x P. alba). Their cell-specific expression was analyzed throughout poplar in source tissues, such as mature leaves, and in sink tissues, such as the wood and bark of the stem, roots and the shoot apex. PtaSULTR1;1 mRNA was detected in companion cells of the transport phloem, in the phloem of high-order leaf veins and in fine roots. PtaSULTR3;3a mRNA was found exclusively in the transport phloem and here in both, companion cells and sieve elements. Both sulfate transporter transcripts were located in xylem parenchyma cells indicating a role for PtaSULTR1;1 and PtaSULTR3;3a in xylem unloading. Changes in mRNA abundance of these and of the sulfate transporters PtaSULTR4;1 and PtaSULTR4;2 were analyzed over an entire growing season. The expression of PtaSULTR3;3a and of the putative vacuolar efflux transporter PtaSULTR4;2 correlated negatively with the sulfate content in the bark. Furthermore, the expression pattern of both PtaSULTR3;3a and PtaSULTR4;2 correlated significantly with temperature and day length. Thus both SULTRs seem to be involved in mobilization of sulfate during spring: PtaSULTR4;2 mediating efflux from the vacuole and PtaSULTR3;3a mediating loading into the transport phloem. In contrast, the abundance of PtaSULTR1;1 and PtaSULTR4;1 transcripts was not affected by environmental changes throughout the whole season. The transcript abundance of all tested sulfate transporters in leaves was independent of weather conditions. However, PtaSULTR1;1 abundance correlated negatively with sulfate content in leaves, supporting its function in phloem loading. Taken together, these findings indicate a transcriptional regulation of sulfate distribution in poplar trees.

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Expression and activity of sulfate transporters and APS reductase in curly kale in response to sulfate deprivation and re-supply

Cited by 50 publications

References 39 publications

A Comparison of Sulfate and Selenium Accumulation in Relation to the Expression of Sulfate Transporter Genes in Astragalus Species

A Comparison of Sulfate and Selenium Accumulation in Relation to the Expression of Sulfate Transporter Genes in Astragalus Species

Sulfur Nutrition in Crop Plants

Seasonal and cell type specific expression of sulfate transporters in the phloem of Populus reveals tree specific characteristics for SO4 2− storage and mobilization

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