Expression analysis of stress responsive pathway genes linked to drought hardiness in an adapted crop, finger millet (Eleusine coracana)

Parvathi, M. S.; Nataraja, K.; Yashoda, B. K.; Ramegowda, H.V.; Mamrutha, H. M.; Rama, Nicolas

doi:10.1007/s13562-012-0135-0

Cited by 45 publications

(16 citation statements)

References 47 publications

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“…Initially, gene transcript analysis from drought tolerant crop species was adopted as a good approach for gene discovery, which has been primarily achieved by general gene expression analysis that is clearly reflected even in recent studies. [24][25][26] Later on, identification of the differentially expressed genes were successfully done by different approaches like subtractive hybridization, suppressive subtractive hybridization, 27 differential display, [28][29] cDNA-AFLP, 30 microarray technology and other means. 22,31 The microarray analysis of Arabidopsis thaliana genome has provided a powerful and widely used method to research the effects of various gene expressions which is also reflected in diverse plant species.…”

Section: Prospecting the Trait Regulatory Genesmentioning

confidence: 99%

Emerging tools, concepts and ideas to track the modulator genes underlying plant drought adaptive traits: An overview

Nataraja

2016

Plant Signaling & Behavior

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Crop vulnerability to multiple abiotic stresses is increasing at an alarming rate in the current global climate change scenario, especially drought. Crop improvement for adaptive adjustments to accomplish stress tolerance requires a comprehensive understanding of the key contributory processes. This requires the identification and careful analysis of the critical morpho-physiological plant attributes and their genetic control. In this review we try to discuss the crucial traits underlying drought tolerance and the various modes followed to understand their molecular level regulation. Plant stress biology is progressing into new dimensions and a conscious attempt has been made to traverse through the various approaches and checkpoints that would be relevant to tackle drought stress limitations for sustainable crop production.

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Section: Prospecting the Trait Regulatory Genesmentioning

confidence: 99%

Emerging tools, concepts and ideas to track the modulator genes underlying plant drought adaptive traits: An overview

Nataraja

2016

Plant Signaling & Behavior

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“…Finger millet seedlings were grown in petri plates under control condition for four days and subsequently subjected to rapid desiccation (air-dried), heat (42C) and cold (4C) stresses for 3 h. To induce salinity and oxidative stress, finger millet seedlings were exposed to NaCl (150 mM) and methyl viologen (10 M) respectively, for 3 h. At the end of the stress period, shoot and root tissues were collected for total RNA isolation and subsequent cDNA synthesis. Drought stress was imposed on 20-day-old plants raised in battery pots by gravimetric approaches 15,16 . Soil field capacities (FCs) of 80%, 60%, 40% and 20% were maintained to create drought stress by controlled irrigation 15,16 .…”

Section: Primersmentioning

confidence: 99%

“…Drought stress was imposed on 20-day-old plants raised in battery pots by gravimetric approaches 15,16 . Soil field capacities (FCs) of 80%, 60%, 40% and 20% were maintained to create drought stress by controlled irrigation 15,16 . Eight days post-stress imposition, leaf tissues from both stressed and non-stressed plants were collected for expression analysis.…”

Section: Primersmentioning

confidence: 99%

“…Representation of CIPK31-like gene was observed in the finger millet drought-specific cDNA library developed in a previous study 15 . Hence, we cloned EcCIPK31-like from finger millet exposed to drought stress.…”

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confidence: 98%

“…Finger millet (Eleusine coracana (L.) Gaertn. ), a drought-tolerant monocot crop, can serve as an ideal system to prospect superior genes associated with tolerance 14,15 . In the present study, we identify and validate a novel drought-responsive CIPK from finger millet.…”

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Full-Length Cloning and Characterization of Abiotic Stress Responsive <i>CIPK31-Like</i> Gene from Finger Millet, a Drought-Tolerant Crop

Nagarjuna¹,

Parvathi²,

Sajeevan³

et al. 2016

Current Science

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Regulatory proteins such as protein kinases are known to play a crucial role in plant stress acclimation pathways. In this study, we report the identification and characterization of an abiotic stress responsive protein kinase called CBL Interacting Protein Kinase (EcCIPK31-like) from finger millet, a drought-tolerant crop. PCR-based approach was followed to clone 1350 bp coding region that encodes a 449 amino acid protein with a calculated molecular mass of 50.69 kDa. The conserved domain analysis revealed the presence of CIPK domain with 90% sequence similarity with fox tail millet SiCIPK31-like gene. We also report its upregulation under salinity, desiccation, oxidative and temperature stresses at seedling level in finger millet. Drought stress at wholeplant level significantly induced the expression of EcCIPK31-like, indicating that the gene is linked to drought signalling pathways. The stress-responsive nature of EcCIPK31-like to diverse stresses indicates that the gene could regulate multiple cellular tolerance traits and its further functional validation can highlight the relevance in abiotic stress acclimation in plants.

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Production and cultivation dynamics of millets in India

Yadav,

Singh,

Kumari

et al. 2024

Crop Science

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Millets gained a great attention at the global level in 2023 which is celebrated as the “International Year of Millets” to create awareness to eventually promote consumption and production. An attempt is made here to understand the cultivation and production dynamics of millets as influenced by their demand and supply. We also assess challenges and emerging opportunities to make millets more productive, competitive, and relevant to future farming. Millets registered a 60% decline in area and a 200% rise in productivity, but production has remained the same during the last seven decades. The demand of millets decreased as food due to greater incentives (purchasing grain at a pre‐determined price) and policies (distribution of grains to the public at a subsidized rate) in favor of wheat and rice. Millets would play a greater role in future agriculture due to challenges posed by climate change, limited water supply, and reduced agro‐biodiversity. This would need a much greater intensity of investment in millet research and adequate support as extended to wheat and rice. Increased emphasis on their genetic improvement and agronomic management is required to develop cultivars, using new tools and technologies, with high production potential and adequate environmental adaptation to make millets competitive with other crops. Mainstreaming the nutritional traits in millet breeding is also critically important to develop high‐yielding cultivars with improved grain quality traits. Promoting millet consumption would remain the key issue for increasing their demand as food, feed, and industrial raw materials through policies and awareness programs. Strengthening of value chain will help in diversifying agri‐food production system and creating an ecosystem for millet promotion.

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Expression analysis of stress responsive pathway genes linked to drought hardiness in an adapted crop, finger millet (Eleusine coracana)

Cited by 45 publications

References 47 publications

Emerging tools, concepts and ideas to track the modulator genes underlying plant drought adaptive traits: An overview

Emerging tools, concepts and ideas to track the modulator genes underlying plant drought adaptive traits: An overview

Full-Length Cloning and Characterization of Abiotic Stress Responsive <i>CIPK31-Like</i> Gene from Finger Millet, a Drought-Tolerant Crop

Production and cultivation dynamics of millets in India

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