Expression analysis of key genes of phenylpropanoid pathway and phenol profiling during Ricinus communis–Fusarium oxysporum f. sp. ricini interaction

Jadhav, Pritam R.; Mahatma, M. K.; Mahatma, Lalit; Jha, Sanjay Kumar; Parekh, Vipulkumar; Khandelwal, Vikas

doi:10.1016/j.indcrop.2013.08.022

Cited by 22 publications

(7 citation statements)

References 18 publications

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“…The fresh leaf and root samples (500 mg) were powdered in liquid nitrogen and extracted at room temperature for 20 min with 2 mL of 80% methanol solution (v/v). 68 The extract was filtered and evaporated to dryness under nitrogen stream in a turbo evaporator (Biotage, TurboVap LV). Immediately before analysis, the extracts were redissolved in 100 μL of absolute methanol.…”

Section: Methodsmentioning

confidence: 99%

Metabolomic Profiling of Drought-Tolerant and Susceptible Peanut (Arachis hypogaea L.) Genotypes in Response to Drought Stress

2020

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Peanut is frequently constrained by extreme environmental conditions such as drought. To reveal the involvement of metabolites, TAG 24 (drought-tolerant) and JL 24 (drought-sensitive) peanut genotypes were investigated under control and 20% PEG 6000-mediated water scarcity conditions at the seedling stage. Samples were analyzed by gas chromatography–mass spectrometry (GC–MS) to identify untargeted metabolites and targeted metabolites, i.e., polyamines and polyphenols by high-performance liquid chromatography (HPLC) and ultrahigh-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS), respectively. The principal component analysis (PCA), partial least-squares discriminant analysis (PLS-DA), heat map, and cluster analysis were applied to the metabolomics data obtained by the GC–MS technique to determine the important metabolites for drought tolerance. Among 46 resulting metabolites, pentitol, phytol, xylonic acid, d-xylopyranose, stearic acid, and d-ribose were important drought-responsive metabolites. Agmatine and cadaverine were present in TAG 24 leaves and roots, respectively, during water-deficit conditions and believed to be the potential polyamines for drought tolerance. Polyphenols such as syringic acid and vanillic acid were produced more in the leaves of TAG 24, while catechin production was high in JL 24 during stress conditions. Seven metabolic pathways, namely, galactose metabolism, starch and sucrose metabolism, fructose and mannose metabolism, pentose and glucuronate interconversion, propanoate metabolism, amino sugar and nucleotide sugar metabolism, and biosynthesis of unsaturated fatty acids were significantly affected by water-deficit conditions. This study provides valuable information about the metabolic response of peanut to drought stress and metabolites identified, which encourages further study by transcriptome and proteomics to improve drought tolerance in peanut.

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Section: Methodsmentioning

confidence: 99%

Metabolomic Profiling of Drought-Tolerant and Susceptible Peanut (Arachis hypogaea L.) Genotypes in Response to Drought Stress

2020

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“…Several studies have reported that disease-resistant genotypes of crops are rich in phenolic acids (Sztejnberg et al, 1983;Bily et al, 2003;He, 2018). Jadhav et al (2013) reported higher concentrations of ferulic acid in infected and non-infected resistant genotypes of castor (Ricinus communis), suggesting a critical role of phenols in castor disease resistance. Zhang et al (2007) and Dong et al (2014) found that the organic acid concentrations in pea root exudates showed a positive relation with disease resistance.…”

Section: Discriminant Compounds In Root Exudatesmentioning

confidence: 99%

“…The phenylpropanoid metabolism pathway plays an important role in plant disease resistance and defense responses (Hammerschmidt, 1999), and the main defensive compounds, such as phenolics, phytoalexin, lignin, and flavonoids, need to be synthesized through this pathway (Jadhav et al, 2013). Pathogen infection usually activates phenylpropane metabolism in plants to enhance host resistance (Gong et al, 1995;Slatnar et al, 2010).…”

Section: Possible Disease Resistance Signal Pathway Associated With Rmentioning

confidence: 99%

Root Exudates Metabolic Profiling Suggests Distinct Defense Mechanisms Between Resistant and Susceptible Tobacco Cultivars Against Black Shank Disease

et al. 2020

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“…Identification of phenolic acids and flavonols using LC-MS/MS Extraction of phenolic acids and flavonols from leaves Phenolic compounds were extracted following the method described by Jadhav et al (2013) with minor modifications. One gram of groundnut leaves were immersed in 80% HPLC grade methanol in screw cap glass tubes and kept in refrigerator at 4 °C for 48 h. Leaves were homogenized and extracted with 5 ml of 80% methanol solution (v/v) at room temperature and centrifuged at 10,000 rpm for 10 min.…”

Section: Targeted Metabolite Profilingmentioning

confidence: 99%

“…Various defense-related and signal metabolites such as salicylic acid, linoleic acid, thymol, pentitol, scopolin, hydroquinone and cinnamic acid were observed higher in tolerant/resistant genotypes during various fungal disease in castor and groundnut (Jadhav et al 2013;Mahatma et al 2018Mahatma et al , 2019. The hypersensitive response signal molecules salicylic acid and jasmonic acid; the antioxidants like glutathione and ascorbic acid are also induced during various host-pathogen interaction to activate plant defense mechanism (Bao et al2016;Li et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling for dissection of late leaf spot disease resistance mechanism in groundnut

Mahatma

Thawait

Jadon

et al. 2021

Physiol Mol Biol Plants

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Late leaf spot (LLS) caused by fungi Passalora personata is generally more destructive and difficult to control than early leaf spot. The aim of this study was to decipher biochemical defense mechanism in groundnut genotypes against P. personata by identifying resistance specific biomarkers and metabolic pathways induced during host-pathogen interaction. Metabolomics of non-infected and infected leaves of moderately resistant (GPBD4 and ICGV86590), resistant (KDG128 and RHRG06083) and susceptible (GG20, JL24 and TMV2) genotypes was carried out at 5 days after infection (65 days after sowing). Nontargeted metabolite analysis using GC-MS revealed total 77 metabolites including carbohydrates, sugar alcohols, amino acids, fatty acids, polyamines, phenolics, terpenes and sterols. Variable importance in projection (VIP) measure of partial least squares-discriminant analysis (PLS-DA) showed that resistant and moderately resistant genotypes possessed higher intensities of ribonic acid, cinnamic acid, malic acid, squalene, xylulose, galactose, fructose, glucose, b-amyrin and hydroquinone while susceptible genotypes had higher amount of gluconic acid 2-methoxime, ribo-hexose-3-ulose and gluconic acid. Heat map analysis showed that resistant genotypes had higher intensities of b-amyrin, hydroquinone in non-infected and malic acid, squalene, putrescine and 2,3,4-trihydroxybutyric acid in infected leaves. Dendrogram analysis further separated resistant genotypes in the same cluster along with infected moderately resistant genotypes. The most significant pathways identified are: linoleic acid metabolism, flavone and flavonol biosynthesis, cutin, suberin and wax biosynthesis, pentose and glucuronate interconversions, starch and sucrose metabolism, stilbenoid biosynthesis and ascorbate and aldarate metabolism. Targeted metabolite analysis further confirmed that resistant genotypes possessed higher content of primary metabolites sucrose, glucose, fructose, malic acid and citric acid. Moreover, resistant genotypes possessed higher content of salicylic, coumaric, ferulic, cinnamic, gallic acid (phenolic acids) and kaempferol, quercetin and catechin (flavonols). Thus metabolites having higher accumulation in resistant genotypes can be used as biomarkers for screening of LSS resistant germplasm. These results unravel that higher amount of primary metabolites leads to stimulate the accumulation of more amounts of secondary metabolites such as phenolic acid, flavanols, stilbenes and terpenoids (squalene and b-amyrin) biosynthesis which are ultimately involved in defense mechanism against LLS pathogen.

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Expression analysis of key genes of phenylpropanoid pathway and phenol profiling during Ricinus communis–Fusarium oxysporum f. sp. ricini interaction

Cited by 22 publications

References 18 publications

Metabolomic Profiling of Drought-Tolerant and Susceptible Peanut (Arachis hypogaea L.) Genotypes in Response to Drought Stress

Metabolomic Profiling of Drought-Tolerant and Susceptible Peanut (Arachis hypogaea L.) Genotypes in Response to Drought Stress

Root Exudates Metabolic Profiling Suggests Distinct Defense Mechanisms Between Resistant and Susceptible Tobacco Cultivars Against Black Shank Disease

Metabolic profiling for dissection of late leaf spot disease resistance mechanism in groundnut

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