Expression analysis of jagged genes in zebrafish embryos

Abstract

The interaction of transmembrane Delta and Jagged/Serrate ligands with Notch receptors on neighboring cells is critically involved in cell specification during development. In zebrafish, the early expression of delta but not of jagged genes has been investigated in some detail. We have analyzed the sequence and embryonic expression pattern of the three zebrafish genes jagged1a, jagged1b, and jagged2. These genes, whose transcripts are detectable by in situ hybridization from early somitogenesis, are widely and dynamically expressed in embryos. Coexpression is limited, however, to the notochord and lens (jagged1a and jagged1b) and to the otic vesicle and pronephros (jagged1b and jagged2). Conversely, jagged1a and jagged2, both widely expressed in the central nervous system, are not coexpressed. jagged2 is also detected in the epidermis, newly formed somites, pharyngeal pouches, and pancreatic exocrine anlage and jagged1b in otic placodes and cell clusters close to the pancreatic islet. The similarities of the expression patterns of jagged and delta genes in zebrafish suggest that the Jagged and Delta ligands are functionally redundant or required in specific combinations in many differentiation processes. Developmental Dynamics 233: 638 -645, 2005.

Jag1b is essential for patterning inner ear sensory cristae by regulating anterior morphogenetic tissue separation and preventing posterior cell death

“…In addition, the expression of Jag1b-EGFP fluorescence in the otic vesicle (Fig. 1A, arrows) is consistent with a previous report that jag1b, rather than jag1a, is expressed in the zebrafish otic vesicle (Zecchin et al, 2005).…”

“…At late stages, lateral inhibition directs differentiation of hair cells and supporting cells (Bryant et al, 2002;Haddon et al, 1998;Lanford et al, 1999). One of the Notch ligands, Jag1, is expressed in the prosensory precursors and regulates specification of prosensory domains (Adam et al, 1998;Lewis et al, 1998;Morrison et al, 1999;Murata et al, 2012;Zecchin et al, 2005). In mice carrying point mutations impairing Jag1 function, posterior and sometimes anterior cristae are missing or mispatterned Tsai et al, 2001).…”

Notch Signaling Regulates the Extent of Hair Cell Regeneration in the Zebrafish Lateral Line

“…We also investigated expression of two other receptors (notch1a, notch1b) and four ligands (deltaD, jagged1a, jagged1b, jagged2), some of which are expressed in the developing zebrafish ear, but which expression in neuromasts have not been reported (Haddon et al, 1998a;Zecchin et al, 2005). The three jagged ligand genes, jagged1a, jagged1b, and jagged2, were not expressed in neuromasts 5-7 dpf both with and without neomycin exposure (data not shown).…”

“…Fluorescent in situ hybridization (FISH) was performed at 55°C as published previously (Julich et al, 2005), followed by myosin-VI immunostaining and counterstaining with 4Ј,6Ј-diamidino-2-phenylindole (DAPI) (Invitrogen). Plasmids were cut and transcribed with the following for generating antisense digoxigenin-labeled RNA probes: Pme1/T7 for atoh1a (a gift from Bruce Riley, Texas A&M University, College Station, TX) (Millimaki et al, 2007); SalI/T7 for notch3 (clone NAA32G11; Open Biosystems, Huntsville, AL); XbaI/T7 for notch1a (a gift from Jose CamposOrtega; University of Cologne, Cologne, Germany) (Bierkamp and Campos-Ortega, 1993); SmaI/T3 for notch1b [cDNA in pBS-SK plasmid (Addgene, Cambridge, MA)] (Kortschak et al, 2001); EcoRI/T7 for deltaA and deltaD (a gift from Bruce Appel, Vanderbilt University, Nashville, TN) (Haddon et al, 1998b); ApaI/Sp6 for jagged1a; EcoRV/Sp6 for jagged1b; and SpeI/T7 for jagged2 (all jagged plasmids were gifts from Natasha Tiso, University of Padua, Padua, Italy) (Zecchin et al, 2005).…”

DeltaA/DeltaD Regulate Multiple and Temporally Distinct Phases of Notch Signaling during Dopaminergic Neurogenesis in Zebrafish

“…We analyzed the expression patterns of the four delta ( A-D) (Haddon et al, 1998) and three jagged genes (1a, 1b, and 2a) (Zecchin et al, 2005) during developmental stages of DA neurogenesis (Fig. 5) (supplemental Fig.…”