Expression analysis of genes involved in mitochondrial biogenesis in mice with MPTP-induced model of Parkinson's disease

Rudenok, Margarita M.; Alieva, Anelya Kh.; Starovatykh, J.S.; Нестеров, М. С.; Stanishevskaya, Vera A.; Колачева, А. А.; Ugryumov, M. V.; Slominsky, Petr; Шадрина, М. И.

doi:10.1016/j.ymgmr.2020.100584

Cited by 9 publications

(15 citation statements)

References 63 publications

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“…Models were created as previously described [ 42 , 43 ]. Detailed information is presented in the work performed by our team earlier [ 44 ]. A brief description is provided in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

“…RNA isolation from tissue samples from each animal as well as analysis of mRNA levels using reverse transcription and real-time PCR (TaqMan technology, StepOnePlus™ System, Applied Biosystems, USA) was carried out in accordance with the protocols described previously [ 44 ].…”

Section: Methodsmentioning

confidence: 99%

“…The protocol of statistical and bioinformatic analysis is described in detail in the work carried out in our laboratory earlier [ 44 ].…”

Section: Methodsmentioning

confidence: 99%

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Expression Analysis of Genes Involved in Transport Processes in Mice with MPTP-Induced Model of Parkinson’s Disease

Rudenok

Шадрина

Filatova

et al. 2022

Life

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Processes of intracellular and extracellular transport play one of the most important roles in the functioning of cells. Changes to transport mechanisms in a neuron can lead to the disruption of many cellular processes and even to cell death. It was shown that disruption of the processes of vesicular, axonal, and synaptic transport can lead to a number of diseases of the central nervous system, including Parkinson’s disease (PD). Here, we studied changes in the expression of genes whose protein products are involved in the transport processes (Snca, Drd2, Rab5a, Anxa2, and Nsf) in the brain tissues and peripheral blood of mice with MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)-induced models of PD. We detected changes in the expressions of Drd2, Anxa2, and Nsf at the earliest modeling stages. Additionally, we have identified conspicuous changes in the expression level of Anxa2 in the striatum and substantia nigra of mice with MPTP-induced models of PD in its early stages. These data clearly suggest the involvement of protein products in these genes in the earliest stages of the pathogenesis of PD.

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“…Models were created as previously described [ 42 , 43 ]. Detailed information is presented in the work performed by our team earlier [ 44 ]. A brief description is provided in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Expression Analysis of Genes Involved in Transport Processes in Mice with MPTP-Induced Model of Parkinson’s Disease

Rudenok

Шадрина

Filatova

et al. 2022

Life

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“…In one of the studies, this reduction was accompanied by an increase in splice variants encoding truncated isoforms which can interfere with the activity of the full length variant [ 209 ]. Additionally, a loss of PGC-1α significantly exacerbates cell death in MPTP [ 52 , 210 , 211 , 212 , 213 , 214 ] and α-synuclein-induced cell death [ 192 , 215 , 216 ] models of PD, while overexpression of PGC-1α enhances autophagy and reduces rotenone- and α-synuclein-mediated toxicity in cell culture [ 186 , 192 , 217 ]. PGC-1α overexpression [ 52 , 212 , 218 , 219 , 220 , 221 , 222 ] or stabilization [ 211 ] can also prevent MPTP-mediated neurotoxicity in vivo.…”

Section: Pgc-1α and Transcriptional Dysregulation In Brain Diseasementioning

confidence: 99%

Dysregulation of PGC-1α-Dependent Transcriptional Programs in Neurological and Developmental Disorders: Therapeutic Challenges and Opportunities

McMeekin

Fox

Boas

et al. 2021

Cells

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Substantial evidence indicates that mitochondrial impairment contributes to neuronal dysfunction and vulnerability in disease states, leading investigators to propose that the enhancement of mitochondrial function should be considered a strategy for neuroprotection. However, multiple attempts to improve mitochondrial function have failed to impact disease progression, suggesting that the biology underlying the normal regulation of mitochondrial pathways in neurons, and its dysfunction in disease, is more complex than initially thought. Here, we present the proteins and associated pathways involved in the transcriptional regulation of nuclear-encoded genes for mitochondrial function, with a focus on the transcriptional coactivator peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1α). We highlight PGC-1α’s roles in neuronal and non-neuronal cell types and discuss evidence for the dysregulation of PGC-1α-dependent pathways in Huntington’s Disease, Parkinson’s Disease, and developmental disorders, emphasizing the relationship between disease-specific cellular vulnerability and cell-type-specific patterns of PGC-1α expression. Finally, we discuss the challenges inherent to therapeutic targeting of PGC-1α-related transcriptional programs, considering the roles for neuron-enriched transcriptional coactivators in co-regulating mitochondrial and synaptic genes. This information will provide novel insights into the unique aspects of transcriptional regulation of mitochondrial function in neurons and the opportunities for therapeutic targeting of transcriptional pathways for neuroprotection.

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“…In addition, the mitochondrial genome is widely considered to be a highly informative molecular marker for comparative genomic research [ 24 , 25 ], and is widely used to study phylogenetic relationships due to its maternal inheritance and high evolutionary rate [ 26 ]. There have already been several studies related to mitochondrial gene expression [ 27 , 28 , 29 , 30 ]. Previous studies have already demonstrated that gene expression in M. alternatus was different when infected with B. xylophilus [ 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Mitogenome Analysis of Four Lamiinae Species (Coleoptera: Cerambycidae) and Gene Expression Responses by Monochamus alternatus When Infected with the Parasitic Nematode, Bursaphelenchus mucronatus

Zhang

Guan

Cao

et al. 2021

Insects

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We determined the mitochondrial gene sequence of Monochamus alternatus and three other mitogenomes of Lamiinae (Insect: Coleoptera: Cerambycidae) belonging to three genera (Aulaconotus, Apriona and Paraglenea) to enrich the mitochondrial genome database of Lamiinae and further explore the phylogenetic relationships within the subfamily. Phylogenetic trees of the Lamiinae were built using the Bayesian inference (BI) and maximum likelihood (ML) methods and the monophyly of Monochamus, Anoplophora, and Batocera genera was supported. Anoplophora chinensis, An. glabripennis and Aristobia reticulator were closely related, suggesting they may also be potential vectors for the transmission of the pine wood pathogenic nematode (Bursaphelenchus xylophilus) in addition to M. alternatus, a well-known vector of pine wilt disease. There is a special symbiotic relationship between M. alternatus and Bursaphelenchus xylophilus. As the native sympatric sibling species of B. xylophilus, B. mucronatus also has a specific relationship that is often overlooked. The analysis of mitochondrial gene expression aimed to explore the effect of B. mucronatus on the energy metabolism of the respiratory chain of M. alternatus adults. Using RT-qPCR, we determined and analyzed the expression of eight mitochondrial protein-coding genes (COI, COII, COIII, ND1, ND4, ND5, ATP6, and Cty b) between M. alternatus infected by B. mucronatus and M. alternatus without the nematode. Expression of all the eight mitochondrial genes were up-regulated, particularly the ND4 and ND5 gene, which were up-regulated by 4–5-fold (p < 0.01). Since longicorn beetles have immune responses to nematodes, we believe that their relationship should not be viewed as symbiotic, but classed as parasitic.

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Expression analysis of genes involved in mitochondrial biogenesis in mice with MPTP-induced model of Parkinson's disease

Cited by 9 publications

References 63 publications

Expression Analysis of Genes Involved in Transport Processes in Mice with MPTP-Induced Model of Parkinson’s Disease

Expression Analysis of Genes Involved in Transport Processes in Mice with MPTP-Induced Model of Parkinson’s Disease

Dysregulation of PGC-1α-Dependent Transcriptional Programs in Neurological and Developmental Disorders: Therapeutic Challenges and Opportunities

Mitogenome Analysis of Four Lamiinae Species (Coleoptera: Cerambycidae) and Gene Expression Responses by Monochamus alternatus When Infected with the Parasitic Nematode, Bursaphelenchus mucronatus

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