Expression analysis of drought stress specific genes in Peanut (Arachis hypogaea , L.)

Abstract

Improving drought tolerance through gene manipulation has been of importance for modern agriculture, which requires identification and validation of candidate genes. Prospecting candidate genes from drought adapted crop species is of immense significance. To identify candidate stress responsive genes from adapted crop, we carried out expression analysis of a few drought responsive ESTs from Arachis hypogaea L. (peanut). The expression patterns of nine AhDR (Arachis hypogea drought responsive) clones were analysed under drought. Quantitative reverse transcription PCR analysis revealed stress responsive nature of the selected genes. The clones AhDR 118 (putative cyclin T-like), AhDR185 (aldehyde reductase-like), AhDR193 (cholin kinase-like) and AhDR 76 (proline amino peptidase-like) showed more than five fold increase in expression. Highly upregulated genes analysed for expression pattern against salinity at seedling level indicated that these genes provide cross protection. This paper is the first report indicating the association of peanut genes cyclin T, proline amino peptidase and choline kinase to drought tolerance, and the possible roles of these genes are discussed.

Peanut (Arachis hypogaea L.): A Prospective Legume Crop to Offer Multiple Health Benefits Under Changing Climate

“…The past decade has seen the development and use of transgenic plants as an important approach to modify and enable crops to withstand variable and ever‐changing climatic conditions (Ahanger et al., ). In this context, transgenic approaches have been regarded as very effective for the induction of salt and drought tolerance among peanut plants so as to achieve improved yields (Krishna et al., ; Pandey et al., ; Pruthvi, Rama, Govind, & Nataraja, , ; Sarkar et al., ). Apart from these reports, transgenic peanut plants exhibited higher activities of enzymatic antioxidants that contributed to oxidative stress regulation (Pandurangaiah et al., ).…”

Emerging tools, concepts and ideas to track the modulator genes underlying plant drought adaptive traits: An overview

“…Initially, gene transcript analysis from drought tolerant crop species was adopted as a good approach for gene discovery, which has been primarily achieved by general gene expression analysis that is clearly reflected even in recent studies. [24][25][26] Later on, identification of the differentially expressed genes were successfully done by different approaches like subtractive hybridization, suppressive subtractive hybridization, 27 differential display, [28][29] cDNA-AFLP, 30 microarray technology and other means. 22,31 The microarray analysis of Arabidopsis thaliana genome has provided a powerful and widely used method to research the effects of various gene expressions which is also reflected in diverse plant species.…”

Transgenic tobacco plants constitutively expressing peanut BTF3 exhibit increased growth and tolerance to abiotic stresses

“…; Pruthvi et al . ). BTF3 was initially reported from studies in HeLa cells, as a basal transcription factor involved in initiating transcription by forming a stable complex with RNA polymerase II (Zheng et al .…”

“…In this study, with a focus on basal transcriptional regulators, a novel transcription factor, Basic Transcription Factor 3 (BTF3), was identified and cloned from an abiotic stress-tolerant crop, peanut, since genes from hardy species are believed to be better at imparting stress tolerance (Parvathi et al 2013;Pruthvi et al 2013). BTF3 was initially reported from studies in HeLa cells, as a basal transcription factor involved in initiating transcription by forming a stable complex with RNA polymerase II (Zheng et al 1987).…”