Expression analysis of AFAP1-AS1 and AFAP1 in breast cancer

Dianatpour, Ali; Faramarzi, Sepideh; Geranpayeh, Lobat; Mirfakhraie, Reza; Motevaseli, Elahe; Ghafouri‐Fard, Soudeh

doi:10.3233/cbm-170831

Cited by 21 publications

(25 citation statements)

References 10 publications

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“…Among putative bio markers for lung cancer are long non-coding RNAs (lncRNAs). These heterogeneous types of transcripts have various fundamental functions in gene regulation and are involved in the pathogenesis of human cancers [6][7][8][9][10][11]. A comprehensive study to find the lncRNA and mRNA signature in lung adenocarcinoma samples and normal tissues has identified more than two thousands lncRNAs with differential expression in these two sets of samples [12].…”

Section: Introductionmentioning

confidence: 99%

Expression Analysis of OIP5-AS1 in Non-Small Cell Lung Cancer

Esfandi¹,

Oskooei²,

Taheri³

et al. 2018

Klin Onkol

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Background: Lung cancer as the most fatal cancer of men has prompted researchers to find bio markers for early detection and prognosis. Among the possible bio markers are a group of non-coding transcripts with sizes more than 200 nucleotides called long non-coding RNAs (lncRNAs). Aims: In the present study, we evaluated the expression levels of the lncRNA OIP5 antisense RNA 1 (OIP5-AS1) in 32 non-small cell lung cancer (NSCLC) samples compared with their corresponding adjacent non-cancerous tissue (ANCTs) by means of real-time polymerase chain reaction. The samples were obtained from patients who were admitted at Labbafi-Nejad Hospital during 2015 and 2016. Results: OIP5-AS expression levels was significantly decreased in tumoral tissues compared with ANCTs in total samples and in male subgroup. However, no association was found between relative expression of OIP5-AS1 and clinicopathological data of patients or history of smoking. Expression levels of this lncRNA were not correlated with patients' age. Conclusions: This lncRNA is possibly a novel bio marker of NSCLC in Iranian patients. Future studies are needed to confirm the results of our study in larger sample sizes. Moreover, based on the difference in lung cancer associated risk factors in different populations, population-based studies are needed to explore the role of this lncRNA in the pathogenesis of cancers in each region to design appropriate targeted therapies for each population.

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Section: Introductionmentioning

confidence: 99%

Expression Analysis of OIP5-AS1 in Non-Small Cell Lung Cancer

Esfandi¹,

Oskooei²,

Taheri³

et al. 2018

Klin Onkol

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“…13 Taken together, one can suggest that the effect of AFAP1-AS1 on expression of AFAP1 might be both context-and tissue-dependent. Such a correlation could not be detected in breast cancer tissues or their adjacent non-cancerous tissues.…”

Section: Discussionmentioning

confidence: 97%

“…Such a correlation could not be detected in breast cancer tissues or their adjacent non-cancerous tissues. 13 Taken together, one can suggest that the effect of AFAP1-AS1 on expression of AFAP1 might be both context-and tissue-dependent. Previous reports have shown both suppressive and inducing effects of naturally occurring antisense RNAs on expression of sense genes.…”

Section: Discussionmentioning

confidence: 97%

“…9 Src family kinases participate in numerous signaling pathways, and transmit signals to the cytoskeleton, membrane and nuclear targets. 13 Based on the reported interaction between AFAP1 and Src, and the role of Src in modulation of the BBB integrity, we hypothesized that AFAP1 expression might be altered in the peripheral blood of MS patients. Based on the reported role of vascular endothelial growth factor in disturbing the BBB function after ischemia, Src is expected to participate in BBB leakage as well.…”

Section: Introductionmentioning

confidence: 99%

“…12 We have recently examined expression of AFAP1 and actin filament associated protein 1-antisense 1 (AFAP1-AS1) in breast cancer patients. 13 Based on the reported interaction between AFAP1 and Src, and the role of Src in modulation of the BBB integrity, we hypothesized that AFAP1 expression might be altered in the peripheral blood of MS patients. Furthermore, as lncRNAs have been noticed to take part in MS development, we postulated that AFAP1-AS1 might participate in the MS evolution through modulation of AFAP1 expression.…”

Section: Introductionmentioning

confidence: 99%

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Long non‐coding RNA AFAP1‐AS1 is upregulated in a subset of multiple sclerosis patients

Mazdeh

Eftekharian

Komaki‬

et al. 2019

Clinical & Exp Neuroim

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Objective Multiple sclerosis (MS) is a chronic inflammatory disorder of the central nervous system. Although the exact mechanism of this autoimmune disorder has not yet been identified, several protein coding genes and noncoding transcripts have been shown to be dysregulated in MS patients. Methods In the current study, we assessed expression of actin filament associated protein 1 (AFAP1) and its naturally occurring antisense (actin filament associated protein 1-antisense 1: AFAP1-AS1) in the peripheral blood of MS patients and healthy individuals. Results AFAP1-AS1 was upregulated in male MS patients compared with healthy male controls (P = 0.048). AFAP1 expression was not different between MS patients and controls or between any subgroups of patients and controls. The interaction between disease status and sex was significant in AFAP1-AS1 expression. There was a significant inverse correlation between expression levels of AFAP1 and AFAP1-AS1 in healthy individuals (r = À0.472, P = 0.001), but not MS patients (r = À0.251, P = 0.078). Conclusions The current study implies the role of AFAP1-AS1 dysregulation in the pathogenesis of a certain group of MS patients. Future studies are required to elaborate the underlying mechanism of such dysregulation.

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Prognostic value of lncRNA AFAP1‐AS1 in breast cancer: a meta‐analysis and validated study in Chinese population

Yang,

Huang,

Sheng

et al. 2023

Cancer Reports

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BackgroundLong non encoding RNA (lncRNA) plays a crucial role in breast cancer. However, the prognostic role of AFAP1‐AS1 in breast cancer remains unclear.AimsTo investigate the relationship between the expression of long non‐coding RNA actin filament‐associated protein1 antisense RNA1 (AFAP1‐AS1) and prognosis of breast cancer.Methods and ResultsMeta‐analysis was performed to explore the correlation between AFAP1‐AS1 and breast cancer. The AFAP1‐AS1expression in patients with breast cancer tissue and adjacent normal tissue from 153 patients was determined by qRT‐PCR. Bioinformatics and Cox proportional‐hazards risk model were used to explore the relationship between expression of AFAP1‐AS1 and prognosis. The combined analysis revealed a significant correlation between AFAP1‐AS1 expression and both overall survival (hazard ratios, HR = 2.33, 95%Cl: 1.94–2.81, p < 0.001) as well as disease‐free survival/progression‐free survival (HR = 2.94, 95%CI: 2.35–3.67, p < 0.001). The relation between expression of AFAP1‐AS1 and breast cancer was determined in 153 breast cancer and adjacent normal tissues. The findings revealed a significantly higher AFAP1‐AS1expression levels in breast cancer tissues compared to adjacent normal tissues (p < 0.001). Additionally, patients exhibiting heightened levels of AFAP1‐AS1 expression were correlated with an unfavorable prognosis (HR = 2.35, 95%CI: 1.47–3.74, p < 0.001), which aligns consistently with the findings of the pooled analysis. The subgroup analysis of clinical characteristics revealed a significant association between high expression of AFAP1‐AS1 and TNM stage (HR = 1.72, 95%CI: 1.11–2.65, p = 0.015).ConclusionThis study demonstrated that AFAP1‐AS1 acts as an oncogene and may serve as a novel prognostic marker for breast cancer, particularly in the Chinese population.

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Expression analysis of AFAP1-AS1 and AFAP1 in breast cancer

Cited by 21 publications

References 10 publications

Expression Analysis of OIP5-AS1 in Non-Small Cell Lung Cancer

Expression Analysis of OIP5-AS1 in Non-Small Cell Lung Cancer

Long non‐coding RNA AFAP1‐AS1 is upregulated in a subset of multiple sclerosis patients

Prognostic value of lncRNA AFAP1‐AS1 in breast cancer: a meta‐analysis and validated study in Chinese population

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