Expression Analysis of OIP5-AS1 in Non-Small Cell Lung Cancer

Abstract

Background: Lung cancer as the most fatal cancer of men has prompted researchers to find bio markers for early detection and prognosis. Among the possible bio markers are a group of non-coding transcripts with sizes more than 200 nucleotides called long non-coding RNAs (lncRNAs). Aims: In the present study, we evaluated the expression levels of the lncRNA OIP5 antisense RNA 1 (OIP5-AS1) in 32 non-small cell lung cancer (NSCLC) samples compared with their corresponding adjacent non-cancerous tissue (ANCTs) by means of real-time polymerase chain reaction. The samples were obtained from patients who were admitted at Labbafi-Nejad Hospital during 2015 and 2016. Results: OIP5-AS expression levels was significantly decreased in tumoral tissues compared with ANCTs in total samples and in male subgroup. However, no association was found between relative expression of OIP5-AS1 and clinicopathological data of patients or history of smoking. Expression levels of this lncRNA were not correlated with patients' age. Conclusions: This lncRNA is possibly a novel bio marker of NSCLC in Iranian patients. Future studies are needed to confirm the results of our study in larger sample sizes. Moreover, based on the difference in lung cancer associated risk factors in different populations, population-based studies are needed to explore the role of this lncRNA in the pathogenesis of cancers in each region to design appropriate targeted therapies for each population.

lncRNA OIP5-AS1 targets ROCK1 to promote cell proliferation and inhibit cell apoptosis through a mechanism involving miR-143-3p in cervical cancer

“…Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is a long intergenic noncoding RNA located in human chromosome at 15q15.1 and transcribed in the antisense of OIP5 gene (14)(15)(16). Also, OIP5-AS1 has been known to regulate the progression of multiple malignancies, such as breast cancer (17,18), malignant melanoma (19), osteosarcoma (20,21), lung adenocarcinoma (22)(23)(24), bladder cancer (25), and colorectal cancer (26). However, the role of OIP5-AS1 in CC is still obscure.…”

Expression Analysis of the Long Non-Coding RNA LINC01433 in Lung Cancer

“…In brief, our study did not verify the overexpression of LINC01433 in NSCLC Iranian patients despite the reported oncogenic role of this lncRNA. In our recent study of assessment of OIP5-AS1 expression in lung cancer tissues, we also reported down-regulation of this lncRNA [5], despite the reported oncogenic role of OIP5--AS1 in other studies [10]. Such results necessitate assessment of expression profile of genes in each ethnic group and potentiate the contribution of genetic/ environmental factors in regulation of gene expression in tumoral tissues.…”

“…Their participations in various cellular processes, for instance proliferation, metastasis and stem cell renewal, has potentiated them as dia gnostic biomarkers in NSCLC [4]. We have recently reported dysregulation of some lncRNAs in NSCLC tissues of Iranian patients [5,6]. A previous study, which aimed at identification of lung-cancer-related lncRNAs, has detected alterations in lncRNA profile in the human bronchial epithelial cell line after transformation of cells by antibenzo(a) pyrene-7,8-diol-9,10-epoxide.…”

Long non-coding RNAs as the critical factors during tumor progressions among Iranian population: an overview

“…Moreover, OIP5-AS1 functions as a ceRNA for miR-195-5p to up regulate NOB1 in human hemangioma endothelial cells [149]. It has been observed that there were significant decreased levels of OIP5-AS among a sub population of Iranian non-small cell lung cancer (NSCLC) samples compared with their corresponding normal margins [150]. In contrast with the results among Iranian NSCLC cases, it has been observed that there was OPI5-AS1 up regulation in a sample of Chinese lung adenocarcinoma tissues.…”