Expressed sequence tags from a NaCl-treated Suaeda salsa cDNA library

Wei

Zeitschrift Für Naturforschung C

et al. 2013

Olimarabidopsis pumila is a close relative of the model plant Arabidopsis thaliana but, unlike A. thaliana, it is a salt-tolerant ephemeral plant that is widely distributed in semi-arid and semi-salinized regions of the Xinjiang region of China, thus providing an ideal candidate plant system for salt tolerance gene mining. A good-quality cDNA library was constructed using cap antibody to enrich full-length cDNA with the gateway technology allowing library construction without traditional methods of cloning by use of restriction enzymes. A preliminary analysis of expressed sequence tags (ESTs) was carried out. The titers of the primary and the normalized cDNA library were 1.6 · 10 6 cfu/mL and 6.7 · 10 6 cfu/mL, respectively. A total of 1093 clones were randomly selected from the normalized library for EST sequencing. By sequence analysis, 894 high-quality ESTs were generated and assembled into 736 unique sequences consisting of 72 contigs and 664 singletons. The resulting unigenes were categorized according to the gene ontology (GO) hierarchy. The potential roles of gene products associated with stress-related ESTs are discussed. The 736 unigenes were similar to A. thaliana, A. lyrata, or Thellungiella salsuginea. This research provides an overview of the mRNA expression profi le and fi rst-hand information of gene sequence expressed in young leaves of O. pumila.

Section: Introductionmentioning

confidence: 99%

Construction of a cDNA Library from the Ephemeral Plant Olimarabidopsis pumila and Preliminary Analysis of Expressed Sequence Tags

Wei

Zeitschrift Für Naturforschung C

et al. 2013

“…At a density of 15 plants m −2 , S. salsa can potentially remove more than 3,000 k Na + ha −1 from saline soil, suggesting that it can be used to improve the quality of saline soils (Zhao 1991). Therefore, S. salsa has been intriguing to researchers in environmental sciences (Cui et al 2008;Song et al 2008;Zhang et al 2001). As a halophyte, the differential gene expressions of S. salsa to salinity have been studied in Wang's laboratory (Zhang et al 2001;Li et al 2002;Wang et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, S. salsa has been intriguing to researchers in environmental sciences (Cui et al 2008;Song et al 2008;Zhang et al 2001). As a halophyte, the differential gene expressions of S. salsa to salinity have been studied in Wang's laboratory (Zhang et al 2001;Li et al 2002;Wang et al 2001). However, little is known about the metabolic responses of S. salsa to salinity.…”

Section: Introductionmentioning

confidence: 99%

Salinity-Induced Effects in the Halophyte Suaeda salsa Using NMR-based Metabolomics

Liu

You

et al. 2011

Plant Mol Biol Rep

Suaeda salsa is a native halophyte in saline soils. Salinity is the most important environmental constraint for plant productivity in the Yellow River Delta. In this work, we investigated the salt-induced effects in root of S. salsa exposed to two environmentally relevant salinities for 1 week and 1 month using nuclear magnetic resonance-based metabolomics. Our results indicated that salt stress inhibited the growth of S. salsa and induced significant metabolic responses including decreased amino acids, lactate, 4-aminobutyrate, malate, choline, phosphocholine, and increased betaine, sucrose, and allantoin in root tissues of S. salsa. In addition, salinity exposures upregulated the activities of superoxide dismutase, glutathione S-transferases, peroxidase, catalase, and glutathione peroxidase in the aboveground part of seedlings of S. salsa after exposures. Overall, these results demonstrated the osmotic and oxidative stresses, disturbances in protein biosynthesis/degradation, and energy metabolism in S. salsa exposed to salinities.

“…More than twenty species of Suaeda have been reported for their ability to survive high salt conditions, including S. salsa (Zhang and Zhao 1998), S. maritima (Maathuis et al 1992;Wetson and Flowers 2010), S. aegyptiaca (Askari et al 2006), S. asparagoides (Ayarpadikannan et al 2012), S. fruticosa (Khan and Ungar 1998), S. glauca (Yang et al 2008b, S. physophora (Song et al 2006) and S. corniculata (Wei et al 2012). In particular, recent studies of salt responsive physiology, molecular genetics, proteomics and metabolomics in S. salsa have yielded more information for understanding the complex mechanisms of Suaeda plants' salt response and tolerance (Zhang et al 2001;Lu et al 2003;Pang et al 2005;Han et al 2011;Li et al 2011Li et al , 2011bWu et al 2012). In previous studies of S. salsa, salt treatment caused the up-regulation of aquaporin (Qi et al 2009), vacuolar Na + /H + antiporter (Qiu et al 2007), V-ATPase and V-PPase , indicating that Suaeda can maintain homeostasis in water potential and ion distribution by increasing leaf succulence and compartmenting the ions.…”

Section: Introductionmentioning

confidence: 99%

Integrated proteomics and metabolomics for dissecting the mechanism of global responses to salt and alkali stress in Suaeda corniculata

et al. 2016

Background and aims Soil alkalinity and salinity are two of the most common environmental stress factors that impact plant growth and productivity. S. corniculata is native to the saline-alkali soil of Northeast China which shows a higher alkali tolerance than other suaeda plants. It will be very important to identify the effect of salt and alkali stress on S. corniculata through proteomics and metabolomics analysis to improve understanding of the resistance of plants. Methods S. corniculata seedlings were exposed to salt and alkali stress for 72 h, respectively. Metabolic changes were quantified by conducting MS-based proteomics and NMR-based metabolomics analysis.Results The response of S. corniculata to salt stress was distinct from that of plants subjected to alkali stress at both physiological and molecular levels. The integrated-omic studies identified 22 and 19 differentially expressed proteins and metabolites mainly involved in carbohydrate metabolism and amino acid metabolism. Conclusions On account of the up-regulation of energy metabolism and higher accumulation of organic osmolytes, S. corniculata shows high pH resistance when it suffers alkali stress. These findings provide insights into the different regulatory mechanisms in halophyte S. corniculata response to salt and alkali stresses.