Expressed recombinant cadherins mediate cell sorting in model systems

Nose, Akinao; Nagafuchi, Akira; Takeichi, Masatoshi

doi:10.1016/0092-8674(88)90114-6

Cited by 632 publications

(417 citation statements)

References 22 publications

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“…In an effort to assess the potential involvement of stabilin-2 in the recognition and engulfing of apoptotic and aged cells, stabilin-2 was stably transfected into mouse fibroblast L cells, which evidence no phagocytic activity under normal conditions. L cells have been shown not to express any cadherins 22 and they have the advantage of being able to distinguish stabilin-2-specific binding from other interactions, because they do not interact significantly with any of the normal or apoptotic cells employed in this study. Through selection in G418, four lines were acquired that consistently expressed stabilin-2 on the cell surface ( Figure 1a).…”

Section: Resultsmentioning

confidence: 99%

Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor

et al. 2007

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Rapid phagocytic clearance of apoptotic cells is crucial for the prevention of both inflammation and autoimmune responses. Phosphatidylserine (PS) at the external surface of the plasma membrane has been proposed to function as a general 'eat me' signal for apoptotic cells. Although several soluble bridging molecules have been suggested for the recognition of PS, the PS-specific membrane receptor that binds directly to the exposed PS and provides a tickling signal has yet to be definitively identified. In this study, we provide evidence that stabilin-2 is a novel PS receptor, which performs a key function in the rapid clearance of cell corpses. It recognizes PS on aged red blood cells and apoptotic cells, and mediates their engulfment. The downregulation of stabilin-2 expression in macrophages significantly inhibits phagocytosis, and anti-stabilin-2 monoclonal antibody provokes the release of the anti-inflammatory cytokine, transforming growth factor-b. Furthermore, the results of timelapse video analyses indicate that stabilin-2 performs a crucial function in the rapid clearance of aged and apoptotic cells. These data indicate that stabilin-2 is the first of the membrane PS receptors to provide tethering and tickling signals, and may also be involved in the resolution of inflammation and the prevention of autoimmunity.

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Section: Resultsmentioning

confidence: 99%

Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor

et al. 2007

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“…This lowering in E-cadherin levels may also facilitate endocrine cell clustering and segregation from other epithelial progenitors. Indeed it was previously shown that homotypic adhesion occurs preferentially in cells with similar level and profile of cadherin expression (Nose et al, 1988;Steinberg and Takeichi, 1994). The maintenance of low level of E-cadherin at all stages, even in endocrine cells, suggests a partial EMT.…”

Section: Discussionmentioning

confidence: 97%

Neurogenin3 initiates stepwise delamination of differentiating endocrine cells during pancreas development

Gouzi

Kim

Katsumoto

et al. 2011

Developmental Dynamics

141

124

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During development, pancreatic endocrine cells are specified within the pancreatic epithelium. They subsequently delaminate out of the epithelium and cluster in the mesenchyme to form the islets of Langerhans. Neurogenin3 (Ngn3) is a transcription factor required for the differentiation of all endocrine cells and we investigated its role in their delamination. We observed in the mouse pancreas that most Ngn3-positive cells have lost contact with the lumen of the epithelium, showing that the delamination from the progenitor layer is initiated in endocrine progenitors. Subsequently, in both mouse and chick newly born endocrine cells at the periphery of the epithelium strongly decrease E-cadherin, break-down the basal lamina and cluster into islets of Langerhans. Repression of E-cadherin is sufficient to promote delamination from the epithelium. We further demonstrate that Ngn3 indirectly controls Snail2 protein expression post-transcriptionally to repress E-cadherin. In the chick embryo, Ngn3 independently controls epithelium delamination and differentiation programs. Developmental Dynamics 240:589-604,

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“…Types of cadherin mediate cell sorting through homophilic interactions. Nose et al (1988) showed that when heterogeneous cell populations with different expressions of cadherins were cocultured, aggregation was seen for cells expressing identical cadherins on the surfaces. A similar role of cadherins may enable folliculo-stellate cells to separate from hormone-producing cells and aggregate homologously to construct pseudofollicles.…”

Section: Discussionmentioning

confidence: 99%

“…Numerous types of cadherins have been identified and shown to regulate morphogenesis through homophilic binding (Nose et al, 1988;Takeichi, 1990). In adults, these molecules are essential for stable cell attachments to maintain the systematic tissue structure.…”

mentioning

confidence: 99%

Distinctive localization of N‐ and E‐cadherins in rat anterior pituitary gland

et al. 2006

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In the rat anterior pituitary gland, folliculo-stellate cells aggregate preferably to form pseudofollicles, and each type of hormone-producing cell shows adhesive affinity with particular types of heterologous hormone-producing cells. Distribution of cadherin types in the rat anterior pituitary was examined immunohistochemically to clarify the unique cell arrangements caused by homologous and heterologous affinities among cells. N-and E-cadherins were detected continuously along cell membranes, while P-cadherin was not. N-and E-cadherins showed distinct isolation in localization, with N-cadherins localized in hormone-producing cells of distal and intermediate lobes in various amounts, and E-cadherins limited to folliculo-stellate cells and marginal layer cells facing the residual lumen of Rathke's pouch. A similar distribution of cadherins was observed in cell clusters of primary cultured anterior pituitary cells. These findings suggest that differential expression of cell adhesion molecules may be partially responsible for localization of hormone-producing cells and folliculo-stellate cells.

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Expressed recombinant cadherins mediate cell sorting in model systems

Cited by 632 publications

References 22 publications

Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor

Rapid cell corpse clearance by stabilin-2, a membrane phosphatidylserine receptor

Neurogenin3 initiates stepwise delamination of differentiating endocrine cells during pancreas development

Distinctive localization of N‐ and E‐cadherins in rat anterior pituitary gland

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