Distinctive localization of N‐ and E‐cadherins in rat anterior pituitary gland

Kikuchi, Motoshi; Yatabe, Megumi; Fujiwara, Ken; Takigami, Shu; Sakamoto, Atsushi; Soji, Tśuyoshi; Yashiro, Takashi

doi:10.1002/ar.a.20384

Cited by 20 publications

(15 citation statements)

References 21 publications

(25 reference statements)

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“…E-CAD (CDH1) staining was also present in cells scattered throughout the gland (Fig. 3F, arrows), as previously reported by Kikuchi et al (2006). Double immunostaining of sections with antibodies against the different hormones revealed that E-CAD staining was not associated with the endocrine cell population (data not shown).…”

Section: Cadherin Expression Within the Pituitary Gland And Purified supporting

confidence: 84%

“…In the pituitary, E-and N-cadherins are known to be expressed in both normal and pathological tissues (Ezzat & Asa 2005, Kikuchi et al 2006, and Rubinek et al (2003) reported that pituitary cell-cell contact mediated by N-cadherin regulates GH secretion in vitro. Furthermore, when anterior pituitary cells are dissociated and cultured in suspension, they aggregate and form clusters after several days (Van der Schueren et al 1982, Denef et al 1989, Noda et al 2003; cell adhesion molecules are likely to be key components in this reaggregation process.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of adherens junction protein expression and localization in pituitary cell networks

Chauvet

El-Yandouzi²,

Mathieu³

et al. 2009

Journal of Endocrinology

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Our view of anterior pituitary organization has been altered with the recognition that folliculo-stellate (FS) and somatotroph cell populations form large-scale three-dimensional homotypic networks. This morphological cellular organization may optimize communication within the pituitary gland promoting coordinated pulsatile secretion adapted to physiological needs. The aim of this study was to identify the molecules involved in the formation and potential functional organization and/or signaling within these cell-cell networks. Here, we have focused on one class of cell adhesion molecules, the cadherins, since b-catenin has been detected in the GH cell network. We have characterized, by qPCR and immunohistochemistry, their cellular expression and distribution. We have also examined whether their expression could be modulated during pituitary tissue remodeling. The mouse anterior pituitary has a restricted and cell-type specific repertoire of cadherin expression: cadherin-11 is exclusively expressed in TSH cells; N-cadherin displays a ubiquitous expression pattern but with different levels of expression between endocrine cell types; E-cadherin is restricted to homotypic contacts between FS cells; while cadherin-18 is expressed both in somatotrophs and FS cells. Thus, each cell type presents a defined combinatorial expression of different subsets of cadherins. This cell-type specific cadherin expression profile emerges early during development and undergoes major changes during postnatal development. These results suggest the existence within the anterior pituitary of cell-cell contact signaling based on a defined pattern of cadherin expression, which may play a crucial role in cellular recognition during the formation and fate of pituitary cell homotypic networks.

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Section: Cadherin Expression Within the Pituitary Gland And Purified supporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Characterization of adherens junction protein expression and localization in pituitary cell networks

Chauvet

El-Yandouzi²,

Mathieu³

et al. 2009

Journal of Endocrinology

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“…6). E-cad also is a marker of MCs and FS cells in the rat pituitary (26). In the mouse pituitary, E-cad and SOX2 colocalize in both the embryo (Fig.…”

Section: Sox2 Is Expressed In Embryonic Pituitarymentioning

confidence: 88%

“…After 4 days, pituispheres were found at a density of 1 per 5,000 to 1 per 10,000 cells seeded. The number of cells per pituisphere was 22.7 Ϯ 0.9 (mean Ϯ SEM of 20 spheres; range, [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30]. When cultured in the presence of BrdU for 24 h after the first day of culture, virtually all cells were labeled by day 4, suggesting growth by cell division rather than reaggregation (Fig.…”

Section: Sox9 Expression In the Pituitary Marks Differentiating Progementioning

confidence: 99%

SOX2-expressing progenitor cells generate all of the major cell types in the adult mouse pituitary gland

Fauquier¹,

Rizzoti

Dattani³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

338

447

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The pituitary gland adapts the proportion of each of its endocrine cell types to meet differing hormonal demands throughout life. There is circumstantial evidence that multipotent adult progenitor cells contribute to this plasticity, but these cells have not been identified. Here, we describe a small (<0.05%) population of progenitor cells in the adult pituitary gland. We show that these cells express SOX2, a marker of several early embryonic progenitor and stem cell types, and form ''pituispheres'' in culture, which can grow, form secondary spheres, and differentiate to all of the pituitary endocrine cell types, as well as folliculostellate cells. Differentiation of cells in the pituispheres was associated with the expression of nestin, SOX9, and S100. Cells expressing SOX2 and E-cadherin are found throughout Rathke's pouch (RP) in embryos but persist in the adult gland, mostly in a narrow zone lining the pituitary cleft, but also are scattered throughout the pituitary. However, unlike in embryonic RP, most of these SOX2 ؉ cells in the adult gland also express SOX9 and S100. We suggest that this SOX2 ؉ /SOX9 ؉ population represents transit-amplifying cells, whereas the SOX2 ؉ /SOX9 ؊ cells we identify are multipotent progenitor/stem cells persisting in the adult pituitary.stem cell ͉ folliculostellate cells

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“…Judging from the localization of Eand N-cadherins in the adenohypophysis of adult rat previously reported (Kikuchi et al, 2006), it is conceivable that E-cadherin expression was lost after embryonic day 15 in hormone-producing cells and, conversely, Ncadherin expression was lost after birth in folliculo-stellate cells. It is also notable that marginal layer cells seemed to retain primitive characteristics in cadherin expression throughout histogenesis and were distinguishable from other parts of the adenohypophysis.…”

Section: Discussionmentioning

confidence: 99%

Changes in E‐ and N‐cadherin expression in developing rat adenohypophysis

Kikuchi

Yatabe

Kouki

et al. 2007

The Anatomical Record

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Recently, we showed that hormone-producing cells express N-cadherin, while folliculo-stellate cells and marginal layer cells express E-cadherin in the adult rat anterior pituitary gland. These cells are believed to originate from a single cell population of the adenohypophyseal placode. In the present study, we immunohistochemically examined the divergence of cadherin types during pituitary histogenesis. Pituitary glands were excised from rats of embryonic day 11 (E11) through postnatal day 60 (P60) and paraffin sections were prepared. E-and N-cadherins were immunostained sequentially using monoclonal and polyclonal primary antibodies and fluorescent secondary antibodies. At E11, E-cadherin was expressed over oral epithelium, while N-cadherin expression was limited to the primordium of Rathke's pouch. When Rathke's pouch was formed at E13, E-and N-cadherin were broadly expressed in the entire cell population. N-cadherin was expressed particularly intensely in the layer of cells that faced the lumen. From E14 through E16, the majority of cells expressed both types of cadherins; however, the cell population to become the pars tuberalis expressed N-cadherin but not E-cadherin. From E18 through E20, when many hormone-producing cells appear, the number of cells that expressed N-cadherin alone increased. However, some cell populations in the pars distalis and multilayered marginal cells still expressed both cadherins. After birth, most of the cells came to express only one of the cadherin types. These results may suggest that undetermined adenohypophyseal cells express both E-and N-cadherin, but come to express either E-or N-cadherin during cytogenesis. Anat Rec, 290:486-490, 2007. 2007 Wiley-Liss, Inc.

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Distinctive localization of N‐ and E‐cadherins in rat anterior pituitary gland

Cited by 20 publications

References 21 publications

Characterization of adherens junction protein expression and localization in pituitary cell networks

Characterization of adherens junction protein expression and localization in pituitary cell networks

SOX2-expressing progenitor cells generate all of the major cell types in the adult mouse pituitary gland

Changes in E‐ and N‐cadherin expression in developing rat adenohypophysis

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