Expresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latente

Yareta, José; Galarza, Marco; Capristano, Silvia; Pellón, Oscar; Sánchez, César; Ballon, Jorge; Guio, Heinner

doi:10.17843/rpmesp.2020.371.4468

Cited by 8 publications

(4 citation statements)

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“…10 miR-155 overexpression in LTBI patients as compared to healthy controls can be explored as a biomarker to differentiate LTBI from ATB. 13 BCG inoculation, an integral part of the Immunization programme in India, has also been seen to have an effect at the miRNA level.In BCG-inoculated individuals, 134 microRNAs are differentially-expressed as compared to BCG un-inocluated individuals. In LTBI individuals, there is upregulation of 75 microRNAs and 11 microRNAs downregulated relative to BCG-inoculated individuals.…”

Section: Discussionmentioning

confidence: 99%

Micro-RNA: A potential screening marker for latent tuberculosis

Sharma¹,

Srivastava²,

Sharma³

et al. 2023

IJMMTD

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An ancient disease, Tuberculosis is one of the most challenging infectious disease contributing to mortality and morbidity worldwide. Tuberculosis elimination globally, by 2050, is a mammoth task as Mycobacterial infections have wide range of presentation, from the clinical to the subclinical or latent and pose a diagnostic and therapeutic challenge. The virulence as well as evading property of Mycobacterium tuberculosisMtb) from the host's immune system confers upon it the ability to remain latent in the host cells. This forms the basis of classification of tuberculosis patient as having latent-TBI or active TB. This review focuses on the role of miRNA as biomarkers of LTBI. The aim is to have an overview of the current knowledge about miRNA, its involvement in TB pathogenesis and its role as a reliable tool for diagnosis of latent tuberculosis.miRNA are non-encoding endogenous RNAs which regulate gene expression by directing their target RNA for degradation or translational repression. Degraded RNA are released in the extracellular milieu, are present in various body fluids, such as blood, saliva, and urine, and are biomarkers for a number of diseases including cancer, Parkinsons’ disease, CAD, liver diseases, TB and other infectious diseases. miRNAs are differentially expressed during active TB and LTBI, and therefore can be used as biomarkers of disease progression and response to anti-TB therapy. This will further permit more specific therapeutic interventions in TB management. A thorough search of available literature resources was performed on online databases such as Google Scholar, NCBI, Nature, Research gate, PubMed, Science Direct. It was found that miRNA are promising biomarkers to identify healthy latent TB individuals for further course of action and can be reliable tools for routine use in current clinical practice for specific therapeutic interventions to limit active TB population. They meet the criteria of ideal biomarkers, such as minimally invasive, accessibility, high specificity, and sensitivity.

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Section: Discussionmentioning

confidence: 99%

Micro-RNA: A potential screening marker for latent tuberculosis

Sharma¹,

Srivastava²,

Sharma³

et al. 2023

IJMMTD

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“…[15] miRNA-155 is commonly found in lung tissues and is involved in mediating immune-related processes such as hematopoiesis, tumors, and B-cell and T-cell gradings. [32] By targeted regulation of mRNA and mediation of the expression of various cytokines, miRNA-155 may be involved in the development of lung diseases such as lung cancer, asthma, TB and pulmonary fibrosis. [33] In recent years, miRNA-155 has been found to play an important role in TB infection.…”

Section: Discussionmentioning

confidence: 99%

“…miRNA expression varies between individuals and tissues, and specific miRNAs have been found to have an immunomodulatory effect on cells induced in response to MTB infection [15] . miRNA-155 is commonly found in lung tissues and is involved in mediating immune-related processes such as hematopoiesis, tumors, and B-cell and T-cell gradings [32] . By targeted regulation of mRNA and mediation of the expression of various cytokines, miRNA-155 may be involved in the development of lung diseases such as lung cancer, asthma, TB and pulmonary fibrosis [33] .…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of microRNA-155 in active tuberculosis

Wang

et al. 2021

Medicine

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Background: Tuberculosis (TB) is a preventable and treatable disease, but the increased mortality and morbidity associated with TB continues to be a leading cause of death globally. MicroRNA (miRNA)-155 has been recognized as a marker of many lung diseases. However, the effectiveness of this marker for diagnosing TB remains unclear.Methods: A detailed search (updated on February 6, 2021) of literature published in the Wanfang database, EMBASE, PubMed, CNKI, and Cochrane Library was conducted to identify eligible studies suitable for inclusion in the current research. The positive likelihood ratio, negative likelihood ratio, specificity, area under the curve, sensitivity, and diagnostic odds ratio were used to investigate the diagnostic potential of miRNA-155.Results: A total of 122 studies related to active TB, which completely complied with the inclusion and exclusion criteria of our metaanalysis, were included. The overall results suggested a moderately high diagnostic accuracy and efficacy of miRNA-155, with a specificity of 0.85 (95% confidence interval = 0.77-0.91) and sensitivity of 0.87 (95% confidence interval = 0.76-0.93). The result based on dysregulated status demonstrated that the upregulated group yielded better accuracy and efficacy than the downregulated group. Notably, the accuracy and efficacy of miRNA-155 in pediatric TB were higher than those in adult TB. The results showed that the accuracy and efficacy of miRNA-155 in children were higher than those in adults. Conclusion:The results of the meta-analysis suggested that miRNA-155 could serve as an effective biomarker for identifying active TB.Abbreviations: AUC = area under the curve, CI = confidence interval, DAE = diagnostic accuracy and efficacy, DOR = diagnostic odds ratio, miRNA = microRNA, MTB = Mycobacterium tuberculosis, NLR = negative likelihood ratio, PLR = positive likelihood ratio, QUADAS-2 = quality assessment of diagnostic accuracy studies-2, SEA = subgroup effect analysis, SEN = sensitivity, SPE = specificity, TB = tuberculosis, TE = threshold effect.

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“…These mechanisms encompass the modulation of apoptotic pathways, induction of autophagy, stimulation of IFN-γ, and secretion of TNF-α. Mycobacterium tuberculosis uses a variety of strategies to counteract these regulatory mechanisms and enhance its survival capabilities ( 15–17 ).…”

Section: Introductionmentioning

confidence: 99%

miR-29 as diagnostic biomarkers for tuberculosis: a systematic review and meta-analysis

He,

Xiong,

Huang

2024

Front. Public Health

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BackgroundThe timely diagnosis of tuberculosis through innovative biomarkers that do not rely on sputum samples is a primary focus for strategies aimed at eradicating tuberculosis. miR-29 is an important regulator of tuberculosis pathogenesis. Its differential expression pattern in healthy, latent, and active people who develop tuberculosis has revealed its potential as a biomarker in recent studies. Therefore, a systematic review and meta-analysis were performed for the role of miR-29 in the diagnosis of tuberculosis.MethodsEMBASE, PubMed, CNKI, Web of Science, and Cochrane Library databases were searched utilizing predefined keywords for literature published from 2000 to February 2024.Included in the analysis were studies reporting on the accuracy of miR-29 in the diagnosis of tuberculosis, while articles assessing other small RNAs were not considered. All types of study designs, including case–control, cross-sectional, and cohort studies, were included, whether prospectively or retrospectively sampled, and the quality of included studies was determined utilizing the QUADAS-2 tool. Publication bias was analyzed via the construction of funnel plots. Heterogeneity among studies and summary results for specificity, sensitivity, and diagnostic odds ratio (DOR) are depicted in forest plots.ResultsA total of 227 studies were acquired from the various databases, and 18 articles were selected for quantitative analysis. These articles encompassed a total of 2,825 subjects, primarily sourced from the Asian region. Patient specimens, including sputum, peripheral blood mononuclear cells, cerebrospinal fluid and serum/plasma samples, were collected upon admission and during hospitalization for tuberculosis testing. miR-29a had an overall sensitivity of 82% (95% CI 77, 85%) and an overall specificity of 82% (95% CI 78, 86%) for detecting tuberculosis. DOR was 21 (95% CI 16–28), and the area under the curve was 0.89 (95% CI 0.86, 0.91). miR-29a had slightly different diagnostic efficacy in different specimens. miR-29a showed good performance in both the diagnosis of pulmonary tuberculosis and extrapulmonary tuberculosis. miR-29b and miR-29c also had a good performance in diagnosis of tuberculosis.ConclusionAs can be seen from the diagnostic performance of miR-29, miR-29 can be used as a potential biomarker for the rapid detection of tuberculosis.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=461107.

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Expresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latente

Cited by 8 publications

References 0 publications

Micro-RNA: A potential screening marker for latent tuberculosis

Micro-RNA: A potential screening marker for latent tuberculosis

Diagnostic value of microRNA-155 in active tuberculosis

miR-29 as diagnostic biomarkers for tuberculosis: a systematic review and meta-analysis

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