Exposure to oxyfluorfen-induced hematobiochemical alterations, oxidative stress, genotoxicity, and disruption of sex hormones in male African catfish and the potential to confront by Chlorella vulgaris

Mansour, Abdallah Tageldein; Amen, Rehab M.; Mahboub, Heba H.; Shawky, Sherine; Orabi, Sahar; Ramah, Amany; Hamed, Heba S.

doi:10.1016/j.cbpc.2023.109583

Cited by 8 publications

(6 citation statements)

References 70 publications

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“…In agreement, OFN, FPB, and PFE kill weeds through the overproduction of ROS which causes lipid peroxidation and damage to the cell membrane and finally cell death [20,29,30]. Similar oxidative stress dependant cytotoxic effects were also reported in fish for OFN [22][23][24][25][26][27], and in rat liver, kidney and testis for FPB [32]. However, no cytotoxic potential has been reported for PFE on animals or humans so far [29].…”

Section: Discussionmentioning

confidence: 55%

“…OFN and FPB also induced cytotoxic effects on THLE2 cells but at IC 50 higher than that of PEN. In consistency, OFN inhibited cell viability in fish and other aquatic invertebrates [22][23][24][25][26] and FPB triggers a cytotoxic effect on mammalian cells [31]. On the other hand, to the best of our knowledge, no available study in the literature addressed a cytotoxic potential for PFE on mammalian cells, so far.…”

Section: Discussionmentioning

confidence: 88%

“…OFN main side effects include an increased incidence of liver cancer in mice, inhibition of the protoporphyrinogen IX oxidase enzyme in rats, and a possible carcinogenic influence in humans [21]. It also has ROS-dependent cyto-and geno-toxic effects on fish and other aquatic invertebrates [22][23][24][25][26]. In zebrafish, OFN disrupts hepatic lipid and glucose metabolism during embryonic development and causes hepatocyte mortality by increasing inflammatory factors [27].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Four Herbicides Cytotoxicity on Normal Liver THLE2 Cells

Al-Mathal,

Khaffagy,

Abu El-Magd

2024

Egyptian Journal of Veterinary Sciences

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HE widespread increase in pesticide use is a source of pollution worldwide. Although herbicides have various applications, they nonetheless represent a risk to human health due to their tendency to bioaccumulate in many different environments. The purpose of this research was to investigate the cytotoxic effects of pendimethalin (PEN), oxyfluorfen (OFN), fluazifop-p-butyl (FPB), and pyraflufen ethyl (PFE) herbicides on human normal liver (THLE2) cells through the evaluation of the cell viability (using the MTT assay), apoptosis (using qPCR), cell cycle (using flowcytometry), and oxidative stress status (using biochemical assays). The four herbicides induced cytotoxic effects on THLE2 cells as evidenced by suppressed cell viability with IC50 values as follows: PEN (60.50±3.19 μM), OFN (100.36±4.66 μM), FPB (174.90±6.54 μM) and PFE (186.72±6.82 μM). They also increased apoptosis (high Bax and caspase 3, and low Bcl2 gene expression). However, only PEN and OFN induced cell cycle arrest in G0/G1 and both S and G2/M phases, respectively, with subsequent downregulated expression of cyclin D1, cyclin A2, and cyclindependent kinase 4 (CDK4). Among the four herbicides, only OFN, PFE, and FPB induced oxidative stress damage as revealed by higher levels of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA), inhibited activities of antioxidant enzymes [catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)], and downregulated expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) genes. FPB and PFE had a greater safety margin on human normal THLE2 cells and might be used with less adverse consequences on human and animal health.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Four Herbicides Cytotoxicity on Normal Liver THLE2 Cells

Al-Mathal,

Khaffagy,

Abu El-Magd

2024

Egyptian Journal of Veterinary Sciences

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“…Unfortunately, this pressure has often resulted in farming more fish in limited space, leading to overcrowding and overstretching of the water environments. Contamination of fish culture water with agro-chemicals such as abamectin (Mahboub et al 2022) and oxyfluorfen (Mansour et al 2023) have been documented as sources of stress in fish. Industrial pollutants such as Bisphenol-A (BPA) (Hamed et al 2021) have also been found to cause stress in fish lowering their productivity.…”

Section: Introductionmentioning

confidence: 99%

Scale Cortisol Modulation in Cultured Nile Tilapia (Oreochromis niloticus): Deciphering the Intricate Role in Chronic Stress through Mitogen-Activated Protein Kinase Signaling Pathway Regulation

Mwaura,

Angienda,

Wekesa

et al. 2024

Preprint

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Chronic stress poses a significant challenge to aquaculture production, and cortisol and glucose have traditionally served as stress markers. However, recent doubts have emerged about the reliability of scale cortisol as a chronic stress determinant due to its involvement in calcium homeostasis. While cortisol influences gene expression in the stress response cascade, few studies explore its impact on metabolic pathways in cultured Nile tilapia. This study delves into the relationship between cortisol signatures of chronic stress in Nile tilapia and the regulation of the Mitogen Activated Protein Kinase (MAPK) signalling pathway, crucial for cell processes. Juvenile Nile tilapia were subjected to different ammonia concentrations and stocking densities for 70 days, with assessments of growth performance, stress levels, RNA sequencing, and differential gene expression. The results revealed a positive correlation between stressors and blood glucose, plasma cortisol, and scale cortisol concentrations. Fish in water with 0.8 mg/L ammonia exhibited elevated plasma glucose and cortisol, while those in 1.2 mg/L showed increased scale cortisol. Higher stocking densities also correlated with elevated stress markers. Notably, cortisol levels rose with ammonia concentration and stocking density, negatively impacting growth performance. The MAPK signalling pathway, linked to cell processes, showed significant downregulation with increasing ammonia concentrations, suggesting its sensitivity to stress. Taken together, six genes in this pathway were significantly enriched following ammonia treatment (Padj less than or equal to 0.05) i.e. Dual Specific Protein Phosphatase 1 (DUSP1), Nuclear Hormone Receptor 38 (NHR38), Heat Shock Protein 1 (HSP 72 KDa), Myelocytomatosis oncogene homologue (Mych), Growth arrest and DNA damage inducible alpha a (GADD 45aa) and Mitogen Activated Protein Kinase 4 (MAP2K4). This study contributes to unravelling the intricate relationship between chronic stress, cortisol, and the MAPK pathway in aquaculture, providing insights crucial for optimizing fish welfare and production.

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“…Moreover, heat shock protein 70 was upregulated in the kidney and liver of O. niloticus exposed to OXY [29]. Exposure to sublethal OXY levels resulted in leukopenia, anemia, lymphopenia, monocytopenia, eosinopenia, and hepato-renal damage in C. gariepinus [8,30] and O. niloticus [31]. Accordingly, the need for antistress, antitoxic, and growth enhancers to alleviate the negative impact of stressful conditions has been demonstrated [32].…”

Section: Introductionmentioning

confidence: 99%

Growth Retardation, Oxidative Stress, Immunosuppression, and Inflammatory Disturbances Induced by Herbicide Exposure of Catfish, Clarias gariepinus, and the Alleviation Effect of Dietary Wormwood, Artemisia cina

El-Houseiny

Anter

Arisha

et al. 2023

Fishes

Self Cite

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The present study evaluated the impact of chronic herbicide (oxyfluorfen; OXY) exposure on catfish, Clarias gariepinus, in terms of growth, hematobiochemical parameters, immune response, antioxidant- and immune-related gene expression, and resistance to monogenean parasites, Quadriacanthus aegypticus. In addition, the protective role of Wormwood, Artemisia cina (AC) against OXY exposure through diet inclusion was also analyzed. The catfish fingerlings were exposed to OXY (1.16 mg/L) for 60 days and fed diets without AC supplementation (control) and with 5% AC supplementation. The results demonstrated that exposure to OXY stunted growth; decreased survival, erythrograms and leukograms, serum protein, and acetylcholinesterase; and negatively altered the antioxidant status. On the contrary, AC supplementation significantly reduced OXY’s negative impacts on growth and hematological, biochemical, and antioxidant balance. In addition, exposure to OXY markedly increased levels of biomarkers of hepatorenal damage, stress indicators, and DNA damage, which were alleviated with AC supplementation. OXY exposure induced immunosuppression manifested by a decrease in lysozyme activities, complement c3, nitric oxide levels, and phagocytic activity. Furthermore, exposure to OXY negatively regulated the expression of immune-antioxidant genes (CAT, GPX1, SOD1, GST, and TGF-Β1). However, it upregulated the expression of CYP1a, IL-1β, and TNF-α in the liver, anterior kidney, and intestine of C. gariepinus. Meanwhile, the addition of AC to the OXY-exposed fish diets notably restored immune components and remedied the altered immune-related gene expressions. Likewise, the AC supplementation significantly alleviated the OXY-induced reduction in the fish survival rate after Q. aegypticus challenge. Accordingly, AC dietary supplementation in catfish diets could alleviate the negative impact of exposure to OXY on growth performance, physiological status, and some immune-antioxidant-related gene expression.

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Exposure to oxyfluorfen-induced hematobiochemical alterations, oxidative stress, genotoxicity, and disruption of sex hormones in male African catfish and the potential to confront by Chlorella vulgaris

Cited by 8 publications

References 70 publications

Evaluation of Four Herbicides Cytotoxicity on Normal Liver THLE2 Cells

Evaluation of Four Herbicides Cytotoxicity on Normal Liver THLE2 Cells

Scale Cortisol Modulation in Cultured Nile Tilapia (Oreochromis niloticus): Deciphering the Intricate Role in Chronic Stress through Mitogen-Activated Protein Kinase Signaling Pathway Regulation

Growth Retardation, Oxidative Stress, Immunosuppression, and Inflammatory Disturbances Induced by Herbicide Exposure of Catfish, Clarias gariepinus, and the Alleviation Effect of Dietary Wormwood, Artemisia cina

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