Exposure to lipopolysaccharide induces immune genes in cultured preadipocytes of Atlantic salmon

Skugor, Stanko; Škugor, Adrijana; Todorčević, Marijana; Torgersen, Jacob; Ruyter, Bente; Krasnov, Aleksei

doi:10.1016/j.fsi.2010.07.026

Cited by 13 publications

(8 citation statements)

References 52 publications

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“…There is growing evidence about the importance of fish adipose tissue in immune functions [31,32]. Bacterial lipopolysaccharide (LPS) administration is a well-known model for simulating pathogen invasion in fish [33,34].…”

Section: Introductionmentioning

confidence: 99%

“…Bacterial lipopolysaccharide (LPS) administration is a well-known model for simulating pathogen invasion in fish [33,34]. LPS administration in fish stimulates cytokine (TNFa, IL-6, and IL-1) secretion by monocytes and macrophages and other related genes in adipose tissue [32,34] increases basal lipolysis and downregulates LPL activity in the adipocyte [23].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Regulation of lipid metabolism and peroxisome proliferator-activated receptors in rainbow trout adipose tissue by lipolytic and antilipolytic endocrine factors

Cruz-García

Sánchez-Gurmaches

Monroy

et al. 2015

Domestic Animal Endocrinology

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regulation of lipid metabolism and peroxisome proliferator-activated receptors in rainbow trout adipose tissue by lipolytic and antilipolytic endocrine factors

Cruz-García

Sánchez-Gurmaches

Monroy

et al. 2015

Domestic Animal Endocrinology

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“…The conversion of osteoblasts into adipocytes can also occur when dietary highly unsaturated fatty acid (HUFA) levels are too high during the early stages of the development of marine fish [71]. Salmon pre-adipocytes seem to possess the ability to differentiate into cells of the immune lineage (most probably macrophage-like cells) depending on environmental conditions [74].However, epigenetic cues affecting cellular differentiation during the development of fish have not yet been investigated.…”

Section: Nutrigenomics-programming the Fish Through Nutrition?mentioning

confidence: 99%

Epigenetics—Potential for Programming Fish for Aquaculture?

Moghadam

Mørkøre

Robinson

2015

JMSE

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Epigenetic marks affecting the expression of genes are triggered by environmental stimuli, can persist throughout life or across multiple generations and can affect an individuals phenotype. In recent years there has been a revival of interest about the possible role of epigenetics in affecting complex or quantitative traits. This growing interest is partly driven by the increasing affordability of ultra-high throughput sequencing methods for studying the epigenome. In this review we focus on some of the possible applications of epigenetic knowledge to the improvement of aquaculture. DNA methylation, in which a methyl group is added to the C5 carbon residue of a cytosine by DNA methyltransferase, has been the most widely studied epigenetic mechanism to date, and methods used to obtain and analyse genome-wide DNA methylation data are outlined. The influence of epigenetic processes on the estimation of breeding values and accuracy of genomic selection for genetic improvement of aquatic species is explored. The possibility of tightly controlling nutritional stimuli found to affect epigenetic processes in order to tailor the development of fish for aquaculture is also discussed. Complex experiments will be required in order to gain a better understanding of the role of epigenetics in affecting quantitative traits in fish.

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“…Interactions between metabolism of lipids and immunity remain to be explored in fish. However, we have previously demonstrated that Atlantic salmon pre-adipocytes show an immune response when added the immune stimulator lipopolysaccharide (LPS) to the culture media [19]. Although not known for Atlantic salmon, in in vitro cultured human 3T3-L1 adipocytes, DHA showed anti-inflammatory effects [20].…”

Section: Introductionmentioning

confidence: 99%

DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment

Bou

Torgersen

Østbye

et al. 2020

IJMS

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Adipocytes play a central role in overall energy homeostasis and are important contributors to the immune system. Fatty acids (FAs) act as signaling molecules capable to modulate adipocyte metabolism and functions. To identify the effects of two commonly used FAs in Atlantic salmon diets, primary adipocytes were cultured in the presence of oleic (OA) or docosahexaenoic (DHA) acid. DHA decreased adipocyte lipid droplet number and area compared to OA. The increase in lipid load in OA treated adipocytes was paralleled by an increase in iNOS activity and mitochondrial SOD2-GFP activity, which was probably directed to counteract increase in oxidative stress. Under lipopolysaccharide (LPS)-induced inflammation, DHA had a greater anti-inflammatory effect than OA, as evidenced by the higher SOD2 activity and the transcriptional regulation of antioxidant enzymes and pro- and anti-inflammatory markers. In addition, DHA maintained a healthy mitochondrial structure under induced inflammation while OA led to elongated mitochondria with a thin thread like structures in adipocytes exposed to LPS. Overall, DHA possess anti-inflammatory properties and protects Atlantic salmon against oxidative stress and limits lipid deposition. Furthermore, DHA plays a key role in protecting mitochondria shape and function.

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Exposure to lipopolysaccharide induces immune genes in cultured preadipocytes of Atlantic salmon

Cited by 13 publications

References 52 publications

Regulation of lipid metabolism and peroxisome proliferator-activated receptors in rainbow trout adipose tissue by lipolytic and antilipolytic endocrine factors

Regulation of lipid metabolism and peroxisome proliferator-activated receptors in rainbow trout adipose tissue by lipolytic and antilipolytic endocrine factors

Epigenetics—Potential for Programming Fish for Aquaculture?

DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment

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