Exposure to extremely low-frequency (50Hz) electromagnetic fields enhances adult hippocampal neurogenesis in C57BL/6 mice

Cuccurazzu, Bruna; Leone, Lucia; Podda, Maria Vittoria; Piacentini, Roberto; Riccardi, Elisa; Ripoli, Cristian; Azzena, Gian Battista; Grassi, Claudio

doi:10.1016/j.expneurol.2010.08.022

Cited by 123 publications

(110 citation statements)

References 82 publications

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“…29 It has been shown that the cholinergic activity in the frontal cortex and hippocampus of the rat decreases immediately after exposure to 60 Hz magnetic field with different intensities 30 and 50 Hz ELF-EMF exposure can increase in vivo neurogenesis. 31 Zecca et al 12 reported that prolonged exposure to ELF/EMF increases the level of µ-opioid receptors in the rat brain. Some researchers reported that ELF-EMF altered the anxiety-like behavior in rats.…”

Section: Introductionmentioning

confidence: 99%

Effects of 3 Hz and 60 Hz Extremely Low Frequency Electromagnetic Fields on Anxiety-Like Behaviors, Memory Retention of Passive Avoidance and Electrophysiological Properties of Male Rats

et al. 2016

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IntroductionThe effects of electromagnetic fields on biological organisms have been a controversial and also interesting debate over the past few decades, because modern civilization is overwhelmed by a broad range of electromagnetic fields, including extremely low frequency electromagnetic fields (ELF/EMF). Numerous investigations, from monitoring changes at molecular levels to the behavioral aspects, have been carried out in vitro and in vivo in order to illustrate different effects of ELF/EMF e.g., its impacts on cells, 1 hormones, 2-6 neurophysiological properties and sleep, 7,8 biochemical factors and metabolism, 9-11 pathology, 12,13 DNA damages and chromosome abnormalities, [14][15][16][17][18][19] reproduction and development, 20 and cancer. [21][22][23] On the other hand, the fact that central nervous system (CNS) as a very complicated electrochemical system may be influenced by electromagnetic fields attracts many researches interests. Vázquez-García et al reported that exposure to 60 Hz ELF/EMF can improves social recognition in male rats. The effects of electromagnetic fields on biological organisms have been a controversial and also interesting debate over the past few decades, despite the wide range of investigations, many aspects of extremely low frequency electromagnetic fields (ELF/EMFs) effects including mechanism of their interaction with live organisms and also their possible biological applications still remain ambiguous. In the present study, we investigated whether the exposures of ELF/EMF with frequencies of 3 Hz and 60 Hz can affect the memory, anxiety like behaviors, electrophysiological properties and brain's proteome in rats. Methods: Male rats were exposed to 3 Hz and 60 Hz ELF/EMFs in a protocol consisting of 2 cycles of 2 h/day exposure for 4 days separated with a 2-day interval. Short term memory and anxiety like behaviors were assessed immediately, 1 and 2 weeks after the exposures. Effects of short term exposure were also assessed using electrophysiological approach immediately after 2 hours exposure. Results: Behavioral test revealed that immediately after the end of exposures, locomotor activity of both 3 Hz and 60 Hz exposed groups significantly decreased compared to sham group. This exposure protocol had no effect on anxiety like behavior during the 2 weeks after the treatment and also on short term memory. A significant reduction in firing rate of locus coeruleus (LC) was found after 2 hours of both 3 Hz and 60 Hz exposures. Proteome analysis also revealed global changes in whole brain proteome after treatment. Conclusion: Here, some evidence regarding the fact that such exposures can alter locomotor activity and neurons firing rate in male rats were presented.

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Section: Introductionmentioning

confidence: 99%

Effects of 3 Hz and 60 Hz Extremely Low Frequency Electromagnetic Fields on Anxiety-Like Behaviors, Memory Retention of Passive Avoidance and Electrophysiological Properties of Male Rats

et al. 2016

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“…In some previous reports, cresyl violet staining alone was performed to examine RF-EMF exposure-induced damage of neuronal cells [23,24]. Because the brain is primarily composed of two broad classes of cells, neurons and glial cells [30], discrimination between neuronal cells and glial cells is essential to define the cell type damaged by RF-EMF exposure. Previous reports evaluated a collation between NeuN and cresyl violet staining to detect neurons [31].…”

Section: Resultsmentioning

confidence: 99%

Effects of 915 MHz Radiofrequency Identification Electromagnetic Field Exposure on Neuronal Precursor Cells in the Dentate Gyrus of Adult Rat Brains

Kim¹,

Lee²,

Lee³

et al. 2015

Journal of electromagnetic engineering and science

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To explore the effects of radiofrequency electromagnetic field on the fate of neuronal cells, we investigated whether exposure to 915 MHz radiofrequency identification (RFID) caused morphological changes in neuronal cells in rat hippocampal dentate gyrus (DG). A reverberation chamber was used as a whole-body RFID exposure system. Rats were assigned to two groups: sham-and RFID-exposed groups. Rats in the RFID-exposed group were exposed to RFID at 4 W/kg specific absorption rate (SAR) for 8 hours daily, 5 days per week, for 2 weeks. Morphological evaluation of DG was performed using immunohistochemistry with doublecortin (DCX) as a neuronal precursor cell marker and neuronal nuclei (NeuN) as a mature neuronal cell marker. No significant morphological changes in DCX+ or NeuN+ cells in the DG of RFID-exposed rats were observed. These results suggest that RFID exposure induces no significant change in DCX+ neuronal precursor or NeuN+ neuronal cells in DG of rats.

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“…Associations between exposure to ELF-EMF and possible health hazards to mankind have been investigated by several epidemiological and experimental studies (4)(5)(6)(7)(8). Most of the epidemiological studies have showed that exposure to ELF-EMF increases the incidence of certain types of cancer, especially acute childhood leukemia (9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Extremely Low-Frequency ElectroMagnetic Field (ELF-EMF) With Different Intensities in Rats Hippocampus

Rezaei‐Tavirani

Hasanzadeh

Seyyedi³

et al. 2018

Arch Neurosci

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Background: Our previous study determined the alteration in behavioral test-related memory and movement in male rats exposed to extremely low frequency electromagnetic field (ELF-EMF). The molecular mechanism of the effect of ELF-EMF on brain damage of rat has not yet fully understood, thus the current study investigated the effect of ELF-EMFs on hippocampus of male rats by the proteomics approach. The aim of this study was to investigate the effects of 50 Hz ELF-EMF at 0.5 and 1 mT 50 Hz for 2 and 4 weeks on male rats and determined protein expression of the hippocampus. Methods: Effects of 0.5 and 1 mT exposures for 2 and 4 weeks on protein expression was determined via proteomics techniques. Statistical analysis of proteome was performed using the Progensis Same Spots software. Bioinformatics analysis based on network was used for determining the mechanism. Results: Two-DE electrophoresis gels determined the effect of ELF-EMF on rat hippocampus, indicating that protein expression was overall down-regulated by increasing ELF-EMF intensity and time. The detected differentially expressed proteins between the groups comprised of Sptan1, Dpysl2, Tpi1, Lap3, Vdac1, and Tppp. Almost all were cytoskeletal proteins, most of which were increased by intensity and time. These proteins attributed to impairment in short memory of hippocampus after exposure to ELF-EMF. The GO analysis based on network, enriched two important biological process, cytoskeletal neurons and metabolic process. Conclusions: This study determined the effect of ELF-EMF, which led to change in protein expression related to the cytoskeleton that contributes to major processes in brain damage.

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Exposure to extremely low-frequency (50Hz) electromagnetic fields enhances adult hippocampal neurogenesis in C57BL/6 mice

Cited by 123 publications

References 82 publications

Effects of 3 Hz and 60 Hz Extremely Low Frequency Electromagnetic Fields on Anxiety-Like Behaviors, Memory Retention of Passive Avoidance and Electrophysiological Properties of Male Rats

Effects of 3 Hz and 60 Hz Extremely Low Frequency Electromagnetic Fields on Anxiety-Like Behaviors, Memory Retention of Passive Avoidance and Electrophysiological Properties of Male Rats

Effects of 915 MHz Radiofrequency Identification Electromagnetic Field Exposure on Neuronal Precursor Cells in the Dentate Gyrus of Adult Rat Brains

Proteomic Analysis of Extremely Low-Frequency ElectroMagnetic Field (ELF-EMF) With Different Intensities in Rats Hippocampus

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