Exposure to 50 Hz magnetic field at 100 µT exert no DNA damage in cardiomyocytes

Abstract

The effects of exposure to magnetic fields (MFs) at electric frequencies (50–60 Hz) on carcinogenicity are still in debate. Whether exposure to MFs affects the heart is also a debated issue. This study aimed to determine whether exposure to extremely low frequency MFs (ELF-MFs) induced DNA damage in cardiomyocytes both in vitro and in vivo. Human ventricular cardiomyocytes were exposed to 50 Hz ELF-MF at 100 µT for 1 h continuously or 75 min intermittently. The effects of the treatments were evaluated by DNA damage, redox status changes and relative signal molecular expression. Moreover, ten male Sprague-Dawley rats were exposed to a 50 Hz MF at 100 µT for 7 days, while another 10 rats were sham exposed. The protein levels of p53 and Hsp70 in heart tissue were analyzed by western blot. The results showed that exposure to ELF-MF did not induce DNA damage, changes to cell cycle distribution or increased reactive oxygen species level. No significant differences were detected in p53 and Hsp70 expression level between the ELF-MF and sham-exposure groups both in vitro and in vivo. All these data indicate that MFs at power-frequency may not cause DNA damage in cardiomyocytes.

Examination of the Effect of a 50-Hz Electromagnetic Field at 500 μT on Parameters Related With the Cardiovascular System in Rats

“…Therefore, different experiments with various examinations should be designed to test and verify the effects of EMF exposure on the cardiovascular system. Previously, we found that 100 µT EMF, which is the threshold of public exposure, did not affect the cardiovascular system and other organs (4,(23)(24)(25)(26). In the current study, we elevated the intensity to 500 µT, which is the threshold of occupational exposure, to explore whether it would exert damages in the cardiovascular system in rats.…”

“…In 2016, our group found that exposure to a 50-Hz EMF at 100 µT for 24 weeks did not show any obvious effects on the cardiovascular system in Sprague-Dawley (SD) rats (23). Recently, we also found that 1 h continuous or 75 min intermittent exposure to a 50-HZ EMF at 100 µT caused no DNA damage in cardiomyocytes derived from both humans and rats (4).…”

“…At the same time, the probability of exposure to these EMFs is increasing dramatically (1). It is well-accepted that EMFs can be generated by a variety of appliances, such as transformers, electrical panels, televisions, and electric blankets (2,3), whose frequency is usually 50-or 60-Hz (4). Human-made EMFs, such as nonionizing radiation, are classified into three categories: extremely low-frequency fields (<300-Hz), high-frequency fields in the radio-frequency band (300-Hz to 3 × 10 8 Hz), and microwaves [3 × 10 8 to 3 × 10 11 Hz; (5)(6)(7)].…”

An Investigation Into the Effects of Long‐Term 50‐Hz Power‐Frequency Electromagnetic Field Exposure on Hematogram, Blood Chemistry, Fibrosis, and Oxidant Stress Status in the Liver and the Kidney From Sprague–Dawley Rats

“…The exposure system was identical to that described previously [Ruan et al, 2019;Wang et al, 2019]. The exposure system consisted of an exposure device and a plastic rack.…”

First person – Yong Wang

“…ABSTRACTFirst Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Yong Wang is first author on ‘Exposure to a 50 Hz magnetic field at 100 µT exerts no DNA damage in cardiomyocytes’, published in BiO. Yong is a PhD student in the lab of Dr Chen at the Huazhong University of Science and Technology, Wuhan, China, investigating cardiovascular pathophysiology.…”