Exposure of the murine RAW 264.7 macrophage cell line to hydroxyapatite dispersions of various composition and morphology: Assessment of cytotoxicity, activation and stress response

Scheel, Julia; Weimans, S.; Thiemann, Astrid; Heisler, Eckhard; Hermann, Martina

doi:10.1016/j.tiv.2009.01.007

Cited by 45 publications

(29 citation statements)

References 38 publications

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“…4B). Physical properties, including density, porosity and crystallization, are probably other key factors in cell viability [25,26]. The SEM images of sintered FHAP particles (Fig.…”

Section: Characterization Of Hydroxyapatite Powdermentioning

confidence: 99%

Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Huang

Hsiao

Chai

2011

Ceramics International

172

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“…4B). Physical properties, including density, porosity and crystallization, are probably other key factors in cell viability [25,26]. The SEM images of sintered FHAP particles (Fig.…”

Section: Characterization Of Hydroxyapatite Powdermentioning

confidence: 99%

Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Huang

Hsiao

Chai

2011

Ceramics International

172

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“…[8][9][10] Our current studies also found that silica NPs could significantly augment proinflammatory and procoagulant responses in ECs through monocyte-EC interactions. 11 However, to the best of our knowledge, most studies are still focused on the direct biological response of engineered HANPs to monocultures of cells in the endothelium or the immune system; 5,[12][13][14][15][16][17][18] as such, the coculture of monocytes and ECs with HANPs has received little attention in this regard.…”

Section: Introductionmentioning

confidence: 99%

Potential proinflammatory effects of hydroxyapatite nanoparticles on endothelial cells in a monocyte–endothelial cell coculture model

Liu¹,

Sun²

2014

IJN

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Currently, synthetic hydroxyapatite nanoparticles (HANPs) are used in nanomedicine fields. The delivery of nanomedicine to the bloodstream exposes the cardiovascular system to a potential threat. However, the possible adverse cardiovascular effects of HANPs remain unclear. Current observations using coculture models of endothelial cells and monocytes with HANPs to mimic the complex physiological functionality of the vascular system demonstrate that monocytes could play an important role in the mechanisms of endothelium dysfunction induced by the exposure to HANPs. Our transmission electron microscopy analysis revealed that both monocytes and endothelial cells could take up HANPs. Moreover, our findings demonstrated that at a subcytotoxic dose, HANPs alone did not cause direct endothelial cell injury, but they did induce an indirect activation of endothelial cells, resulting in increased interleukin-6 production and elevated adhesion molecule expression after coculture with monocytes. The potential proinflammatory effect of HANPs is largely mediated by the release of soluble factors from the activated monocytes, leading to an inflammatory response of the endothelium, which is possibly dependent on p38/c-Jun N-terminal kinase, and nuclear factor-kappa B signaling activation. The use of in vitro monocyte–endothelial cell coculture models for the biocompatibility assessment of HANPs could reveal their potential proinflammatory effects on endothelial cells, suggesting that exposure to HANPs possibly increases the risk of cardiovascular disease.

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“…Needle-shaped HA also slowed down the cell metabolism and inhibited hatching in catfish T-cells and zebrafish embryos in a dose-dependent manner [63]. Other studies suggested good biocompatibility and a lack of cytotoxic effects of needle-shaped HA when tested in vitro [64,65], demonstrating a great degree of variability of the biological response to such morphologies. The exact amount, shape, texture, aspect ratio, surface charge, agglomeration degree, the dosage mode (seeding cells onto the powder, depositing powder on top of the cells or co-seeding), the cell type and the interaction of the particles with biomolecules contained in the growth medium are all factors influencing the biological response to them, needing further studies to be discerned and analyzed.…”

Section: Resultsmentioning

confidence: 99%

Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies

Stojanović

Ignjatović

et al. 2016

Materials Science and Engineering: C

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Recent developments in bone tissue engineering have led to an increased interest in one-dimensional (1D) hydroxyapatite (HA) nano- and micro-structures such as wires, ribbons and tubes. They have been proposed for use as cell substrates, reinforcing phases in composites and carriers for biologically active substances. Here we demonstrate the synthesis of 1D HA structures using an optimized, urea-assisted, high-yield hydrothermal batch process. The one-pot process, yielding HA structures composed of bundles of ribbons and wires, was typified by the simultaneous occurrence of a multitude of intermediate reactions, failing to meet the uniformity criteria over particle morphology and size. To overcome these issues, the preparation procedure was divided to two stages: dicalcium phosphate platelets synthesized in the first step were used as a precursor for the synthesis of 1D HA in the second stage. Despite the elongated particle morphologies, both the precursor and the final product exhibited excellent biocompatibility and caused no reduction of viability when tested against osteoblastic MC3T3-E1 cells in 2D culture up to the concentration of 2.6 mg/cm2. X-ray powder diffraction combined with a range of electron microscopies and laser diffraction analyses was used to elucidate the formation mechanism and the microstructure of the final particles. The two-step synthesis involved a more direct transformation of DCP to 1D HA with the average diameter of 37 nm and the aspect ratio exceeding 100:1. The comparison of crystalline domain sizes along different crystallographic directions showed no signs of significant anisotropy, while indicating that individual nanowires are ordered in bundles in the b crystallographic direction of the P63/m space group of HA. Intermediate processes, e.g., dehydration of dicalcium phosphate, are critical for the formation of 1D HA alongside other key aspects of this phase transformation, it must be investigated in more detail in the continuous design of smart HA micro- and nano-structures with advanced therapeutic potentials.

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Exposure of the murine RAW 264.7 macrophage cell line to hydroxyapatite dispersions of various composition and morphology: Assessment of cytotoxicity, activation and stress response

Cited by 45 publications

References 38 publications

Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Potential proinflammatory effects of hydroxyapatite nanoparticles on endothelial cells in a monocyte–endothelial cell coculture model

Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies

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Exposure of the murine RAW 264.7 macrophage cell line to hydroxyapatite dispersions of various composition and morphology: Assessment of cytotoxicity, activation and stress response

Cited by 45 publications

References 38 publications

Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Potential proinflammatory effects of hydroxyapatite nanoparticles on endothelial cells in a monocyte&ndash;endothelial cell coculture model

Hydrothermally processed 1D hydroxyapatite: Mechanism of formation and biocompatibility studies

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Potential proinflammatory effects of hydroxyapatite nanoparticles on endothelial cells in a monocyte–endothelial cell coculture model