Exploring the virome of Vasconcellea x heilbornii: the first step towards a sustainable production program for babaco in Ecuador

Cornejo-Franco, Juan F.; Medina-Salguero, Andres X.; Flores, Francisco; Chica, Eduardo J.; Grinstead, Samuel; Mollov, Dimitre; Quito-Avila, Diego F.

doi:10.1007/s10658-020-02037-2

Cited by 17 publications

(21 citation statements)

References 16 publications

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“…The terminal regions 5'-AAATAAAACATCT and -CTCTTAGAATGAG-3' were conserved in both isolates. Similar sequence identities (95-96%) were observed between the newly sequenced genomes and a PRSV isolate (MH974109) from a mountain papaya species, known as babaco (Vasconcellea x heilbornii), recently reported from Ecuador [31].…”

Section: Plos Onesupporting

confidence: 78%

Genetic characterization of a mild isolate of papaya ringspot virus type-P (PRSV-P) and assessment of its cross-protection potential under greenhouse and field conditions

Medina-Salguero

Cornejo-Franco

Grinstead³

et al. 2021

PLoS ONE

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A mild isolate of Papaya ringspot virus type-P, abbreviated as PRSV-mild, from Ecuador was sequenced and characterized. The most distinguishing symptom induced by PRSV-mild was gray powder-like leaf patches radiating from secondary veins. In greenhouse experiments, PRSV-mild did not confer durable protection against a severe isolate of the virus (PRSV-sev), obtained from the same field. Furthermore, isolate specific detection in mixed-infected plants showed that PRSV-sev becomes dominant in infections, rendering PRSV-mild undetectable at 90–120 days post superinfection. Virus testing using isolate-specific primers detected PRSV-mild in two out of five surveyed provinces, with 10% and 48% of incidence in Santo Domingo and Los Ríos, respectively. Comparative genomics showed that PRSV-mild lacks two amino acids from the coat protein region, whereas amino acid determinants for asymptomatic phenotypes were not identified. Recombination events were not predicted in the genomes of the Ecuadorean isolates. Phylogenetic analyses placed both PRSV-mild and PRSV-sev in a clade that includes an additional PRSV isolate from Ecuador and others from South America.

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Section: Plos Onesupporting

confidence: 78%

Genetic characterization of a mild isolate of papaya ringspot virus type-P (PRSV-P) and assessment of its cross-protection potential under greenhouse and field conditions

Medina-Salguero

Cornejo-Franco

Grinstead³

et al. 2021

PLoS ONE

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“…Debido a su gran diversidad, es posible encontrar especies portadoras de genes de resistencia a enfermedades, tolerantes a bajas temperaturas y poseedores de atributos organolépticos especiales (Manshardt y Wenslaff, 1989;Drew et al, 1998;Scheldeman et al, 2007). Dentro de esa amplia diversidad, destaca Vasconcellea x heilbornii, cuyos frutos son apreciados en los mercados internacionales debido a su sabor y aroma únicos, además de la ausencia de semillas (Cornejo et al, 2020). Debido a esta característica, su propagación es realizada mediante estacas, el cual es un método poco confiable debido al riesgo de transmisión de enfermedades (Posada, 2005;Jádan et al, 2016a;Cornejo et al, 2020).…”

Section: Volumen 33 (2021)unclassified

“…Dentro de esa amplia diversidad, destaca Vasconcellea x heilbornii, cuyos frutos son apreciados en los mercados internacionales debido a su sabor y aroma únicos, además de la ausencia de semillas (Cornejo et al, 2020). Debido a esta característica, su propagación es realizada mediante estacas, el cual es un método poco confiable debido al riesgo de transmisión de enfermedades (Posada, 2005;Jádan et al, 2016a;Cornejo et al, 2020).…”

Section: Volumen 33 (2021)unclassified

Establecimiento y multiplicación in vitro de papayas de montaña: Vasconcellea chachapoyensis Y Vasconcellea x Heilbornii

Lapiz-Culqui

Tejada-Alvarado

Meléndez-Mori

et al. 2021

Bioagro

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La aplicación de las técnicas de cultivo de tejidos en especies de Vasconcellea es una alternativa viable para la propagación de estas especies. Sin embargo, la eficiencia de estas herramientas está condicionada por factores como la contaminación de explantes, el efecto de los reguladores de crecimiento y las especies que se desea propagar. El presente estudio tuvo como objetivo evaluar protocolos de desinfección superficial, con diferentes concentraciones de NaClO y Ca(ClO)2 y tiempos de inmersión, para el establecimiento de explantes. Asimismo, evaluar la acción de las fitohormonas BAP, ANA y AG3 en diferentes concentraciones en la multiplicación in vitro de segmentos nodales de Vasconcellea chachapoyensis y Vasconcellea x heilbornii. Los experimentos se condujeron bajo un diseño completamente al azar con arreglo factorial y los datos se analizaron con la prueba de Kruskal Wallis. Los resultados muestran que al utilizar NaClO al 1,5 % por 10 min se alcanzó solo 10 % de contaminación, siendo V. chachapoyensis la especie que logró mayor viabilidad de explantes en la etapa de establecimiento. Por otro lado, el medio suplementado con BAP generó mayor número de brotes y hojas, mientras que el AG3 indujo mayor elongación de los brotes. Además, cabe mencionar que la especie influyó en la multiplicación, siendo V. x heilbornii la de mejor desarrollo morfológico. Estos resultados señalan la eficacia e importancia de los desinfectantes y reguladores de crecimiento durante el cultivo de tejidos, y representan una alternativa propicia para la micropropagación de especies del género Vasconcellea.

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“…Taken together, babaco has great potential to become a major product in the fruit market. However, the crop is seriously affected by pests and diseases caused by fungi, bacteria, nematodes, and several viruses including babaco mosaic virus (BabMV) [9,10].…”

Section: Introductionmentioning

confidence: 99%

Phylodynamics and Coat Protein Analysis of Babaco Mosaic Virus in Ecuador

Mosquera-Yuqui¹,

Flores

Moncayo

et al. 2022

Plants

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Babaco is a fast-growing herbaceous shrub with great commercial potential because of the organoleptic properties of its fruit. Babaco mosaic virus (BabMV) is a potexvirus in the family Alphaflexiviridae affecting babaco in all the provinces that produce this crop in Ecuador. BabMV was recently described but it has been affecting babaco for decades and, since many potexviruses are serologically indistinguishable, it may have been previously misidentified as papaya mosaic virus. Based on the coat protein (CP) gene, we aimed to study the distribution and epidemiological patterns of BabMV in babaco and chamburo over the years and to model its three-dimensional structure. Sequences of the CP were obtained from thirty-six isolates from plants collected in the main babaco-producing provinces of Ecuador between 2016 and 2021. The evolution rate of BabMV was estimated at 1.21 × 10−3 nucleotide substitutions site−1 year−1 and a time of origin of the most recent common ancestor around 1958.80. From molecular dynamics simulations, compared to other proteins of BabMV—RDRP, TGB1, and Alkb domain—the CP exhibited a higher flexibility with the C and N terminals as the most flexible regions. The reconstructed viral distribution provides dispersion patterns which have implications for control approaches of BabMV.

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Exploring the virome of Vasconcellea x heilbornii: the first step towards a sustainable production program for babaco in Ecuador

Cited by 17 publications

References 16 publications

Genetic characterization of a mild isolate of papaya ringspot virus type-P (PRSV-P) and assessment of its cross-protection potential under greenhouse and field conditions

Genetic characterization of a mild isolate of papaya ringspot virus type-P (PRSV-P) and assessment of its cross-protection potential under greenhouse and field conditions

Establecimiento y multiplicación in vitro de papayas de montaña: Vasconcellea chachapoyensis Y Vasconcellea x Heilbornii

Phylodynamics and Coat Protein Analysis of Babaco Mosaic Virus in Ecuador

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