Exploring the structural determinants of selective phosphopeptide recognition using bivalent metal-coordination complexes

Abstract

We demonstrate that Lewis acidic coordination complexes equipped with cationic binding groups might be best utilized as selective receptors for binding phosphopeptides with anionic side chain residues proximal to the phosphorylated residue.

Zinc Ion-immobilized Magnetic Microspheres for Enrichment and Identification of Multi-phosphorylated Peptides by Mass Spectrometry

“…31,32 In particular, the binuclear ZnDpa complex has been shown to coordinate phosphate groups with high affinity and selectivity. [33][34][35][36][37][38][39][40][41] Here, we devised the binuclear ZnDpa complex-coated magnetic microspheres (ZnMMs) for the selective enrichment of phosphopeptides. In order to improve the selectivity of phosphopeptide enrichment and to take advantage of easy isolation from complex biological samples, we introduced two key modifications to the conventional IMAC adsorbent.…”

A novel rGR–TiO2–ZrO2 composite nanosheet for capturing phosphopeptides from biosamples

“…18) is higher than that of conventional IMAC beads. Such metal oxides, which have amphoteric characteristics and show Lewis acid and base behavior at different solution pH values, 19 lead to reversible bridging bidentate binding between the amphoteric metal surface of the metal oxides and the phosphate groups. Recently, rare-earth materials have been used to capture and label phosphopeptides selectively based on their hydrolytic catalytic ability, the high affinities of rare-earth ions to phosphate moieties of phosphopeptides, and their large specic surface areas.…”