A novel rGR–TiO₂–ZrO₂ composite nanosheet for capturing phosphopeptides from biosamples

Abstract

A novel rGR–TiO2–ZrO2 composite nanosheet was synthesized and applied to selectively capture phosphopeptides from complex biosamples.

A guanidyl-functionalized TiO2 nanoparticle-anchored graphene nanohybrid for enhanced capture of phosphopeptides

“…[11][12][13][14] Recently, it has been proved that guanidyl groups could be regarded as a better functionalized group to improve the binding of the phosphate groups and prevent the binding of other peptides. [15][16][17] Inspired by this, the guanidyl groups are modied on the TiO 2 , which will offer higher capacity of phosphopeptides binding due to the interaction between the phosphate groups and guanidyl groups. 18,19 The aim of this work is to design and synthesize a novel MOAC material with guanidyl-functionalized TiO 2 nanoparticles (GF-TiO 2 ) anchored on the surface of graphene oxide (GO) platform (denoted as GF-TiO 2 -GO) for selective enrichment of phosphopeptides.…”

“… 11–14 Recently, it has been proved that guanidyl groups could be regarded as a better functionalized group to improve the binding of the phosphate groups and prevent the binding of other peptides. 15–17 Inspired by this, the guanidyl groups are modified on the TiO 2 , which will offer higher capacity of phosphopeptides binding due to the interaction between the phosphate groups and guanidyl groups. 18,19 …”

Design and synthesis of magnetic binary metal oxides nanocomposites through dopamine chemistry for highly selective enrichment of phosphopeptides

“…and they all show different preference for different phosphopeptides. According to the reports in the literature, APs integrating binary metal oxides (BMOs) can significantly improve the enrichment efficiency ,which triggered our interest to develop new BMOAPs for highly selective enrichment of phosphopeptides. Among all the metal oxides that are widely used in phosphoproteomics research, TiO 2 and ZrO 2 demonstrate outstanding enrichment ability toward phosphopeptides.…”

Nanomaterials in Proteomics

“…1436 phosphorylated sites corresponding to 622 phosphopeptides (163 mono‐ and 459 multiphosphopeptides) were identified from the identical mouse brain extracts. Moreover, Huang et al integrated TiO 2 and ZrO 2 via sol–gel and hydrothermal treatment method, and identified 1980 and 577 phosphopeptides from the extracts of mouse brain and mouse liver, respectively . Liu et al integrated Ti and Zr into MOFs to fabricate binary MOFs with Zr–O and Ti–O units (Figure B), and identified 44 phosphopeptides (34 mono‐ and 10 multiphosphopeptides) from 1 µL of human saliva .…”