Design and synthesis of magnetic binary metal oxides nanocomposites through dopamine chemistry for highly selective enrichment of phosphopeptides

Mengyi, Wang; Sun, Xueni; Li, Yan; Deng, Chunhui

doi:10.1002/pmic.201500277

Cited by 28 publications

(10 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In summary, the on‐chip magnetic SPE‐MALDI‐TOF MS system with In 2 O 3 ‐coated Fe 3 O 4 MNPs as the adsorbent led to high selectivity, good reusability, and low detection limit when used for the detection of phosphopeptides. In addition, a comparison of the present system with some reported methods employing various magnetic metal oxides revealed that the present approach provided comparable selectivity, sensitivity, rapidness, and reagent dosage [, ].…”

Section: Resultsmentioning

confidence: 94%

“…In recent years, there have been various methods to enrich phosphopeptides including ion exchange chromatography, immune affinity capture, IMAC, and metal oxide affinity chromatography (MOAC) . Among these techniques, ion exchange chromatography is difficult to be used for complex biological samples because of the low efficiency and huge sample loss.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Highly selective enrichment of phosphopeptides by on‐chip indium oxide functionalized magnetic nanoparticles coupled with MALDI‐TOF MS

Jiang

Song

et al. 2017

Proteomics

View full text Add to dashboard Cite

Selective and efficient preconcentration is indispensable for low concentration of phosphopeptides in phosphorylated protein-related samples prior to MS-based analysis. Herein, an on-chip system coupled magnetic SPE with MALDI-TOF MS was designed. A metal oxide affinity chromatography material, indium oxide, was coated on the surface of Fe O magnetic nanoparticles to prepare the adsorbent, spatially confined with an applied magnetic field. The adsorbent exhibited high selectivity for phosphopeptides in tryptic digests of the mixture of β-casein and BSA (1:1000) and the mixture of β-casein, BSA, and ovalbumin (1:100:100). Thanking to the enrichment ability and specificity for phosphopeptides with the adsorbent, the on-chip magnetic SPE-MALDI-TOF MS approach showed high sensitivity with a low detection limit of 4 fmol. In addition, the developed approach was used to analyze phosphopetides in non-fat milk digests and human serum successfully.

show abstract

Section: Resultsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Highly selective enrichment of phosphopeptides by on‐chip indium oxide functionalized magnetic nanoparticles coupled with MALDI‐TOF MS

Jiang

Song

et al. 2017

Proteomics

View full text Add to dashboard Cite

show abstract

“…Enrichment : Polycatecholamines have been used as a component in the enrichment of a range of biological species from biological samples for subsequent analysis. This includes the enrichment of phosphopeptides based on their strong binding to titanium (IV) ions, hafnium (IV) ions, zirconium‐based metal–organic frameworks, and binary metal oxides of titanium (IV) and zirconium (IV) . Analysis has been conducted in situ using competitive binding of phosphopeptides and a fluorescent probe onto coated particles, the release of the probe stopping quenching due to close association with polydopamine and providing a fluorescent indication of phosphopeptide concentration .…”

Section: Applications Of Catecholamine Polymersmentioning

confidence: 99%

“…Analysis has been conducted in situ using competitive binding of phosphopeptides and a fluorescent probe onto coated particles, the release of the probe stopping quenching due to close association with polydopamine and providing a fluorescent indication of phosphopeptide concentration . These phosphopeptide binding species were also immobilized onto magnetic composites subsequently used for magnetic solid phase extraction of the enriched of phosphopeptides for analysis using mass spectrometry . Magnetic solid phase extraction of modified polydopamine coated magnetic nanomaterials has also been used for other selective separations of glycopeptide, and histidine‐tagged proteins from complex biological samples and estrogens from aqueous solution .…”

Section: Applications Of Catecholamine Polymersmentioning

confidence: 99%

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Barclay

Hegab

Clarke

et al. 2017

Adv Materials Inter

294

223

View full text Add to dashboard Cite

Almost ten years ago, Lee et al. [13] formulated a universal adhesive coating based on observation of the strong attachment by mussels through their byssal threads to virtually all types of inorganic and organic surfaces. The amino acid compositions of the mussel foot proteins that generate the attachment are rich in catechol and amine groups. [13][14][15] These groups associate under marine conditions, forming strong covalent and noncovalent interactions with substrates. [13] This led to the idea that both catechol and amine groups were crucial in forming robust, wide spectrum adhesive nanolayers [16,17] and consequently dopamine was conceived as a powerful building block for spontaneous deposition of thin polymer films. The dopamine monomer is deposited onto unadulterated surfaces through self-assembly and oxidative cross-linking from mild pH aqueous solution in a rapid reaction. This results in a durable, nanoscale, conformal, and hydrophilic coating that can be readily modified to introduce specific surface chemistries for a particular application. [18][19][20][21][22] These bioinspired, polydopamine materials are chemically and structurally indistinguishable from eumelanins found in the human body, [23,24] providing excellent biocompatibility. Additionally, polydopamine has broad electromagnetic absorption and excellent photothermal energy conversion [25] as well as having ionic-electronic hybrid conductivity. [26] Along with the excellent coating performance, these properties provide a vast array of potential applications for polydopamine materials.In this article, we explain what is known about polydopamine and related catecholamine coatings; their formation, the adhesion mechanism, and their physicochemical properties and we also review the applications of these materials (Figure 1). Since 2011, there have been a number of reviews on this subject matter, including general reviews on the physicochemical properties of polydopamine coatings [11,20,[27][28][29] and their applications. [20,27,30] There are also a number of more specific reviews on the chemical synthesis and modulation of polycatecholamine coatings, [31] the biomedical applications of these coatings, [8,[32][33][34] their electronic properties, [26,35] the use of polydopamine to make films at fluid interfaces, [36] in hierarchically constructed particles, [33] and capsules, [30] exploitation of polycatecholamine coatings in membrane filtration, [37] polydopamine-derived carbon materials, [38] and the use of coordination chemistry in catechol-based materials. [39] Nonetheless, this area of research is growing so rapidly [25,27] that many recent Polydopamine and related polycatecholamines can be easily deposited onto almost any surface from mild, aqueous solution. This results in durable, nanoscale coatings that exhibit high biocompatibility and have useful chemical and electronic properties. Additionally, these materials can be readily chemically and physically modified, and consequently, they are used extensively for surface modification. This ...

show abstract

“…A total of 349 phosphorylated sites corresponding to 170 phosphopeptides (66 mono‐ and 104 multiphosphopeptides) were identified from mouse brain extracts. Furthermore, our group integrated Zr and Ti into a whole ( Figure A), which was denoted as magG/PD/(Zr‐Ti)O 4 . 1436 phosphorylated sites corresponding to 622 phosphopeptides (163 mono‐ and 459 multiphosphopeptides) were identified from the identical mouse brain extracts.…”

Section: Nanomaterials In Phosphoproteomicsmentioning

confidence: 99%

Nanomaterials in Proteomics

Sun

Shen

et al. 2019

Adv Funct Materials

Self Cite

View full text Add to dashboard Cite

For a deep understanding of the human proteome, the first crucial step is always the effective separation of targeted proteins/peptides from complex samples. In mass spectrometry‐based proteomics, nanomaterials have emerged as a highly effective means to separate targeted proteins/peptides and increase their relative abundance, which is beneficial to conduct mass spectrometric analysis. In particular, nanomaterials with different specific functionalities have received great attention in ordinary proteomics, phosphoproteomics, and glycoproteomics, for which the easily recognizable characteristics of these proteomes take the primary responsibility, such as the hydrophobicity, phosphate groups, cis–diol structure, and hydrophilicity. This review mainly focuses on the recent design and preparation of nanomaterials with specific recognition ability, as well as their application in the aforementioned three types of proteomics.

show abstract

Design and synthesis of magnetic binary metal oxides nanocomposites through dopamine chemistry for highly selective enrichment of phosphopeptides

Cited by 28 publications

References 21 publications

Highly selective enrichment of phosphopeptides by on‐chip indium oxide functionalized magnetic nanoparticles coupled with MALDI‐TOF MS

Highly selective enrichment of phosphopeptides by on‐chip indium oxide functionalized magnetic nanoparticles coupled with MALDI‐TOF MS

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Nanomaterials in Proteomics

Contact Info

Product

Resources

About