Exploring the role of wastewater-based epidemiology in understanding tuberculosis burdens in Africa

Mtetwa, Hlengiwe Nombuso; Amoah, Isaac Dennis; Kumari, Sheena; Bux, Faizal; Reddy, Poovendhree

doi:10.1016/j.envres.2023.115911

Cited by 4 publications

(3 citation statements)

References 51 publications

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“…Identification of M. bovis strains ( SB0130 and SB1474 ) in human samples highlights the necessity for enhanced surveillance and control in communities with high HIV/AIDS prevalence and significant wildlife-livestock-human interfaces, at risk of zoonotic TB [ 6 , 16 ]. The coexistence of diverse susceptible hosts in shared ecosystems poses control challenges, necessitating a One Health approach.…”

Section: Discussionmentioning

confidence: 99%

“…Remarkably, to date, in SA there have been no documented reports of M. bovis in people [ 13 ]. This observation is indeed perplexing, especially when considering increased reports of M. bovis infections in people from at-risk communities in neighbouring African countries [ 14 , 15 ], the high prevalence of HIV/AIDS among SA populations, the well-established presence of M. bovis in SA wildlife and livestock, and the recent surge in environmental reports documenting its presence in wastewater [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification and molecular characterization of Mycobacterium bovis DNA in GeneXpert® MTB/RIF ultra-positive, culture-negative sputum from a rural community in South Africa

Goosen,

Moodley,

Ghielmetti

et al. 2024

One Health

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and molecular characterization of Mycobacterium bovis DNA in GeneXpert® MTB/RIF ultra-positive, culture-negative sputum from a rural community in South Africa

Goosen,

Moodley,

Ghielmetti

et al. 2024

One Health

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“…The information presented in this table has been sourced from the studies conducted by Mtetwa et al [ 15 ] and Mtetwa et al [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

Optimisation of analytical methods for tuberculosis drug detection in wastewater: A multinational study

Mtetwa,

Amoah,

Kumari

et al. 2024

Heliyon

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Identification and Molecular Characterization of Mycobacterium bovis DNA in GeneXpert® MTB/RIF Ultra-Positive, Culture-Negative Sputum Samples from a Rural Community in South Africa.

Goosen,

Moodley,

Ghielmetti

et al. 2023

Preprint

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This study investigated the presence of Mycobacterium bovis (M. bovis) DNA in human sputum samples collected from residents who reside adjacent to the M. bovis-endemic Hluhluwe-iMfolozi wildlife park, South Africa (SA). Sixty-eight sputum samples were GeneXpert MTB/RIF Ultra-positive for M. tuberculosis complex (MTBC) DNA but culture negative for M. tuberculosis. Amplification and Sanger sequencing of hsp65 and rpoB genes from residual frozen sputum confirmed the presence of detectable amounts of MTBC in DNA extracted from 20 out of the 68 sputum samples. Region of difference PCR, spoligotyping and gyrB long-read amplicon deep sequencing together identified M. bovis (n = 10) from the 20 samples from which MTBC DNA could be detected. Notably, M. bovis spoligotypes SB0130 and SB1474 were identified in 4 samples, with SB0130 previously identified in local cattle and wildlife and SB1474 exclusively in African buffaloes in the adjacent park. M. bovis DNA in sputum, from people living near the park, underscores zoonotic transmission potential in SA. Identification of spoligotypes specifically associated with wildlife only and spoligotypes found in livestock as well as wildlife, highlights the complexity of TB epidemiology at wildlife-livestock-human interfaces. These findings emphasize the need for integrated surveillance and control strategies to curb potential spillover and for the consideration of human M. bovis infection in SA patients with positive Ultra results.

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Exploring the role of wastewater-based epidemiology in understanding tuberculosis burdens in Africa

Cited by 4 publications

References 51 publications

Identification and molecular characterization of Mycobacterium bovis DNA in GeneXpert® MTB/RIF ultra-positive, culture-negative sputum from a rural community in South Africa

Identification and molecular characterization of Mycobacterium bovis DNA in GeneXpert® MTB/RIF ultra-positive, culture-negative sputum from a rural community in South Africa

Optimisation of analytical methods for tuberculosis drug detection in wastewater: A multinational study

Identification and Molecular Characterization of Mycobacterium bovis DNA in GeneXpert® MTB/RIF Ultra-Positive, Culture-Negative Sputum Samples from a Rural Community in South Africa.

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