Identification and molecular characterization of Mycobacterium bovis DNA in GeneXpert® MTB/RIF ultra-positive, culture-negative sputum from a rural community in South Africa

Goosen, Wynand J.; Moodley, Sashen; Ghielmetti, Giovanni; Moosa, Yumna; Zulu, Thando; Smit, Theresa; Kleynhans, Leanie; Kerr, Tanya J.; Streicher, Elizabeth M.; Hanekom, Willem A.; Warren, Robin M.; Wong, Emily B.; Miller, Michele A.

doi:10.1016/j.onehlt.2024.100702

Cited by 2 publications

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Insights into mycobacteriome composition in Mycobacterium bovis-infected African buffalo (Syncerus caffer) tissue samples

Ghielmetti,

Kerr,

Bernitz

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Animal tuberculosis significantly challenges global health, agriculture, and wildlife conservation efforts. Mycobacterial cultures are resource-intensive, time-consuming, and challenged by heterogeneous populations. In this study, we employed a culture-independent approach, using targeted long-read-based next-generation sequencing (tNGS), to investigate the mycobacterial composition in 60 DNA samples extracted from Mycobacterium bovis infected culture-confirmed African buffalo tissue. We detected mycobacterial DNA in 93.3% of the samples and the sensitivity for detecting Mycobacterium tuberculosis complex (MTBC) was 91.7%, demonstrating a high concordance of our culture-independent tNGS approach with mycobacterial culture results. In five samples, we identified heterogenous mycobacterial populations with various non-tuberculous mycobacteria, including members of the Mycobacterium avium complex (MAC), M. smegmatis, and M. komaniense. The latter Mycobacterium species was described in South Africa from bovine nasal swabs and environmental samples from the Hluhluwe-iMfolozi Park, which was the origin of the buffalo samples in the present study. This finding suggests that exposure to environmental mycobacteria may confound detection of MTBC in wildlife. In conclusion, our approach represents a promising alternative to conventional methods for detecting mycobacterial DNA. This high-throughput technique enables rapid differentiation of heterogeneous mycobacterial populations, which will contribute valuable insights into the epidemiology, pathogenesis, and microbial synergy during mycobacterial infections.

show abstract

Insights into mycobacteriome composition in Mycobacterium bovis-infected African buffalo (Syncerus caffer) tissue samples

Ghielmetti,

Kerr,

Bernitz

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

Insights into Mycobacteriome Composition in Mycobacterium bovis-Infected African Buffalo (Syncerus caffer) Tissue Samples: A Culture-Independent Approach

Ghielmetti,

Kerr,

Bernitz

et al. 2024

Preprint

View full text Add to dashboard Cite

Animal tuberculosis significantly challenges global health, agriculture, and wildlife conservation efforts. Mycobacterial cultures necessitate stringent biosafety measures due to the risk of laboratory-acquired infections. In this study, we employed a culture-independent approach, using targeted long-read-based next-generation sequencing (tNGS), to investigate the mycobacterial composition in DNA extracted from Mycobacterium bovis infected culture-confirmed African buffalo tissue. We detected mycobacterial DNA in 93.3% of the samples and the sensitivity for detecting Mycobacterium tuberculosis complex (MTBC) was 91.7%, demonstrating a high concordance of our culture-independent tNGS approach with mycobacterial culture results. We identified heterogenous mycobacterial populations with various non-tuberculous mycobacteria, including members of the Mycobacterium avium complex, M. smegmatis, and M. komaniense. The latter Mycobacterium species was described in South Africa from bovine nasal swabs and environmental samples from the Hluhluwe-iMfolozi Park, which was the origin of the buffalo samples in the present study. This finding suggests that mycobacterial DNA found in the environment may confound detection of MTBC in wildlife. In conclusion, our approach represents an alternative to conventional methods for detecting mycobacterial DNA. This high-throughput technique enables the differentiation of heterogeneous mycobacterial populations and facilitates relative quantification, which will contribute valuable insights into the epidemiology, pathogenesis, and microbial synergy during mycobacterial infections.

show abstract

Identification and molecular characterization of Mycobacterium bovis DNA in GeneXpert® MTB/RIF ultra-positive, culture-negative sputum from a rural community in South Africa

Cited by 2 publications

References 47 publications

Insights into mycobacteriome composition in Mycobacterium bovis-infected African buffalo (Syncerus caffer) tissue samples

Insights into mycobacteriome composition in Mycobacterium bovis-infected African buffalo (Syncerus caffer) tissue samples

Insights into Mycobacteriome Composition in Mycobacterium bovis-Infected African Buffalo (Syncerus caffer) Tissue Samples: A Culture-Independent Approach

Contact Info

Product

Resources

About