Exploring the resistance mechanisms in Trichosporon asahii: Triazoles as the last defense for invasive trichosporonosis

Padovan, Ana Carolina Barbosa; Rocha, Walicyranison Plínio da Silva; Toti, Ana Caroline de Moraes; Jesus, Daniel Felipe Freitas de; Chaves, Guilherme Maranhão; Colombo, Arnaldo Lopes

doi:10.1016/j.fgb.2019.103267

Cited by 35 publications

(14 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As expected, none of the strains was sensitive to echinocandins. In line with findings of the current investigation, studies from different regions, such as South America, Europe and Asia, demonstrated that Trichosporon species have low MICs of triazoles in vitro, but have poor sensitivity to amphotericin B and 5‐fluorocytosine 18‐23 . Low susceptibility to amphotericin B and intrinsic resistance to echinocandins is a major concern because these drugs constitute first‐line empirical therapy for patients with fungaemia.…”

Section: Discussionsupporting

confidence: 86%

MALDI‐TOF MS characterisation, genetic diversity and antifungal susceptibility of Trichosporon species from Iranian clinical samples

Ahangarkani

İlkit

Vaseghi

et al. 2021

Mycoses

View full text Add to dashboard Cite

Background:Trichosporonosis is an emerging fungal infection caused by Trichosporon species, a genus of yeast-like fungi, which are frequently encountered in human infections ranging from mild cutaneous lesions to fungemia in immunocompromised patients. The incidence of trichosporonosis has increased in recent years, owing to higher numbers of individuals at risk for this infection. Although amphotericin B, posaconazole and isavuconazole are generally effective against Trichosporon species, some isolates may have variable susceptibility to these antifungals.Objectives: Herein, we evaluated the species distribution, genetic diversity and antifungal susceptibility profiles of Trichosporon isolates in Iran. Methods:The yeasts were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Phylogenetic analysis was performed based on amplified fragment length polymorphism (AFLP). The in vitro susceptibilities of eight antifungal agents were analysed using the Clinical and Laboratory Standards Institute broth microdilution methods. Results:The isolates belonged to the species T asahii (n = 20), T japonicum (n = 4) and T faecale (n = 3). A dendrogram of the AFLP analysis demonstrated that T asahii | 919 AHANGARKANI et Al.

show abstract

Section: Discussionsupporting

confidence: 86%

MALDI‐TOF MS characterisation, genetic diversity and antifungal susceptibility of Trichosporon species from Iranian clinical samples

Ahangarkani

İlkit

Vaseghi

et al. 2021

Mycoses

View full text Add to dashboard Cite

show abstract

“…T. asahii and P. norvegensis have recently been recognized as emerging fungal pathogens able to cause invasive infections in neutropenic cancer patients as well as in critically ill patients submitted to invasive medical procedures and broad-spectrum antibiotic therapy. 47 , 48 .…”

Section: Discussionmentioning

confidence: 99%

Species Diversity and Antifungal Susceptibilities of Oral Yeasts from Patients with Head and Neck Cancer

Wu¹,

Gan²,

Li³

et al. 2021

IDR

View full text Add to dashboard Cite

Purpose To investigate the colonization and susceptibility to antifungal drugs of oral yeasts in head and neck cancer patients in Hainan, China. Methods Oral mucosa samples from 211 head and neck cancer patients were collected. Oral yeasts were isolated and identified to species by rDNA ITS sequencing. The susceptibilities of all yeasts to amphotericin B, fluconazole, fluorocytosine, itraconazole, and ketoconazole were determined. Results Yeasts were isolated from 124 of the 211 oral swabs. The 124 yeast isolates were classified into following 10 species, from the most frequent to the least frequent, Candida albicans (53.2%), Candida tropicalis (22.6%), Candida krusei (6.5%), Kodamaea ohmeri (5.6%), Candida parapsilosis (4.8%), Hanseniaspora opuntiae (2.4%), Candida metapsilosis (1.6%), Pichia terricola (1.6%), Pichia norvegensis (0.8%), and Trichosporon asahii (0.8%). The overall frequencies of resistance among the yeasts to amphotericin B, fluconazole, flucytosine, itraconazole, and ketoconazole were 4.8%, 8.1%, 16.1%, 9.7%, and 9.7%, respectively. One C. albicans strain and one C. tropicalis strain were tolerant/resistant to all five drugs. Conclusion Given the high prevalence of oral yeast colonization in head and neck cancer patients and the observed resistance of certain yeast isolates to the five antifungal drugs, our results suggest that rapid identification and susceptibility testing should be implemented before antifungal treatment is applied among patients with head and neck cancer in Hainan.

show abstract

“…Although most Trichosporon strains isolated in clinical laboratories are related to episodes of colonization or superficial infections, this fungus has been recognized as an opportunistic agent causing emergent, invasive infections in tertiary care hospitals worldwide. Unfortunately, invasive trichosporonosis is life-threatening with a high mortality rate, and its prognosis in the patient is notably poor [ 11–14 ]. Therefore, accurate interspecies identification and treatment are highly important for trichosporonosis patients.…”

Section: Discussionmentioning

confidence: 99%

Urinary Tract Infections Caused by Fluconazole-Resistant Trichosporon japonicum in 2 Kidney Transplant Patients and Analysis of Their Homology

Huang

Chen

et al. 2020

Open Forum Infectious Diseases

View full text Add to dashboard Cite

Trichosporon spp. are emerging opportunistic agents that cause systemic diseases and life-threatening disseminated disease in immunocompromised hosts. Trichosporon japonicum is a highly rare cause of invasive trichosporonosis. In this study, we described two cases of urinary tract infection caused by Trichosporon japonicum in kidney transplant patients. Culturing of urine samples yielded bluish green colonies of T. japonicum on Candida chromogenic fungal medium. The isolates were identified as T. japonicum by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI TOF-MS, Autof MS 1000). The identification of T. japonicum was further confirmed by 18S rRNA gene sequencing. In vitro drug susceptibility testing showed that the two strains of T. japonicum were resistant to 5-flucytosine, fluconazole and caspofungin with dose-dependent sensitivity to itraconazole and voriconazole but sensitivity to amphotericin B. The homology of the two T. japonicum strains, as determined by cluster analysis and principal component analysis of MALDI-TOF MS, was approximately 85%, suggesting a common nosocomial origin. The first two case reports of fluconazole-resistant T. japonicum urinary infection in kidney transplant recipients are presented.

show abstract

Exploring the resistance mechanisms in Trichosporon asahii: Triazoles as the last defense for invasive trichosporonosis

Cited by 35 publications

References 72 publications

MALDI‐TOF MS characterisation, genetic diversity and antifungal susceptibility of Trichosporon species from Iranian clinical samples

MALDI‐TOF MS characterisation, genetic diversity and antifungal susceptibility of Trichosporon species from Iranian clinical samples

Species Diversity and Antifungal Susceptibilities of Oral Yeasts from Patients with Head and Neck Cancer

Urinary Tract Infections Caused by Fluconazole-Resistant Trichosporon japonicum in 2 Kidney Transplant Patients and Analysis of Their Homology

Contact Info

Product

Resources

About