Exploring the Radial and Longitudinal Aeration of Primary Maize Roots by Means of Clark-Type Oxygen Microelectrodes

Darwent, M. J.; Armstrong, W.; Armstrong, J.; Beckett, P. M.

doi:10.1023/b:rupp.0000003269.31706.ed

Cited by 30 publications

(40 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is clear that such a low oxygen supply from aerated plant parts cannot provide for normal growth of anaerobically incubated roots of these mesophytes. This conclusion agrees well with the results of other researchers, who showed that the length of roots of maize (mesophyte) seedlings did not exceed 10 cm under anaerobic conditions [94]. The aforementioned value (7%) varies considerably as dependent on plant species and age, and on temperature in root zone.…”

Section: Avoidance Of Anaerobiosis At the Whole Plant Level Or The Stsupporting

confidence: 93%

Plant Anaerobic Stress as a Novel Trend in Ecological Physiology, Biochemistry, and Molecular Biology: 1. Establishment of a New Scientific Discipline

Vartapetian

2005

Russ J Plant Physiol

View full text Add to dashboard Cite

This review attempted to follow the establishment of a novel branch of biology arisen at the interfaces between plant physiology, biochemistry, and molecular biology-plant anaerobic stress. Most attention was given to the early period of these investigations, the activity of the members of International Society for Plant Anaerobiosis in particular, and the contribution of Russian scientists, who played a significant role at that time in the establishment and international recognition of this new trend. In this connection, the following points are considered: (1) Crawford's metabolic theory, which could not withstand experimental verification but induced an active discussion, thus stimulating further investigations in this field; (2) a concept of two main strategies of plant adaptation to anaerobic stress (true and apparent adaptation), which was put forward based on the following experimental data: (a) a discovery of a paradoxical phenomenon of hyper-sensitivity, but not hyperresistance to anoxia, of the flood-tolerant plant roots ("apparent" tolerance); (b) the elucidation of the physiological role of oxygen transported from aerated organs of flood-tolerant plants to the roots inhabiting anaerobic environment; (c) demonstration of the key role of both energy metabolism, and (d) substrate providing for glycolysis and ethanolic fermentation in plants manifesting "true" tolerance to oxygen deprivation; (3) the discovery of plant stress proteins; and finally (4) pH-stat theory put forward by Davies.

show abstract

Section: Avoidance Of Anaerobiosis At the Whole Plant Level Or The Stsupporting

confidence: 93%

Plant Anaerobic Stress as a Novel Trend in Ecological Physiology, Biochemistry, and Molecular Biology: 1. Establishment of a New Scientific Discipline

Vartapetian

2005

Russ J Plant Physiol

View full text Add to dashboard Cite

show abstract

“…Polarographic microsensors have proved an extremely useful tool for studying oxygen transport in plant tissues (Ober and Sharp 1996), roots (Armstrong et al 2009), and phytoplankton mats (Dodds et al 1999). Determination of respiratory critical oxygen pressures in roots via measurement of O 2 concentration depends on many parameters, and use of Clark-type microelectrodes (Darwent et al 2003) in conjunction with radial root transport models (Armstrong et al 2009) has provided a great deal of insight into root physiology.…”

Section: Introductionmentioning

confidence: 99%

Self-referencing optrodes for measuring spatially resolved, real-time metabolic oxygen flux in plant systems

McLamore

Jaroch

Chatni

et al. 2010

Planta

View full text Add to dashboard Cite

The ability to non-invasively measure metabolic oxygen flux is a very important tool for physiologists interested in a variety of questions ranging from basic metabolism, growth/development, and stress adaptation. Technologies for measuring oxygen concentration near the surface of cells/tissues include electrochemical and optical techniques. A wealth of knowledge was gained using these tools for quantifying real-time physiology. Fiber-optic microprobes (optrodes) have recently been developed for measuring oxygen in a variety of biomedical and environmental applications. We have adopted the use of these optical microsensors for plant physiology applications, and used the microsensors in an advanced sensing modality known as self-referencing. Self-referencing is a non-invasive microsensor technique used for measuring real-time flux of analytes. This paper demonstrates the use of optical microsensors for non-invasively measuring rhizosphere oxygen flux associated with respiration in plant roots, as well as boundary layer oxygen flux in phytoplankton mats. Highly sensitive/selective optrodes had little to no hysteresis/calibration drift during experimentation, and an extremely high signal-to-noise ratio. We have used this new tool to compare various aspects of rhizosphere oxygen flux for roots of Glycine max, Zea mays, and Phaseolus vulgaris, and also mapped developmentally relevant profiles and distinct temporal patterns. We also characterized real-time respiratory patterns during inhibition of cytochrome and alternative oxidase pathways via pharmacology. Boundary layer oxygen flux was also measured for a phytoplankton mat during dark:light cycling and exposure to pharamacological inhibitors. This highly sensitive technology enables non-invasive study of oxygen transport in plant systems under physiologically relevant conditions.

show abstract

“…The mean and standard deviation of four replicates having different letters within each section of each species indicated that they were significantly different at p ≤ 0.05 according to one-way ANOVA test). (Darwent et al, 2003;Enstone and Peterson, 2005). A thick barrier to prevent ROL was developed in the basal zone of many wetland plants, including Oryza sativa (Colmer, 2002(Colmer, , 2003; Critesion marinum (McDonald, Galwey, and Colmer, 2001a), Lolium multiflorum (McDonald, Galwey, and Colmer, 2002) and Echinochloa crus-galli and Eleocharis acuta (McDonald et al, 2002); while a moderate barrier was induced for species such as Caltha palustris (Visser et al, 2000), Lophopyrum elongatum (McDonald, Galwey, and Colmer, 2001b) and Rumex palustris (Visser et al, 2000) when grown in a stagnant deoxygenated solution.…”

Section: Of Cell Layersmentioning

confidence: 99%

Effect of Wastewater Discharge on Root Anatomy and Radial Oxygen Loss (ROL) Patterns of Three Mangrove Species in Southern China

Tam

Wong

2010

International Journal of Phytoremediation

View full text Add to dashboard Cite

The effects of wastewater discharge on radial oxygen loss (ROL) and root anatomy varied among mangrove species. ROL of Bruguiera gymnorrhiza (L) increased from 22.44 ng cm(-2) min(-1) in the control (just fresh water) to 31.09 ng cm(-2) min(-1) when received normal wastewater (NW) and to 44.22 ng cm(-2) min(-1) when treated with strong wastewater (10NW). However, discharge of both NW and 10NW caused 28% decreases of ROL in the root tip of Excoecaria agallocha L., and the decreases in Acanthus ilicifolius L were even more significant, with 45% when treated by 10NW The changes of ROL were related to the root anatomy. Among three species, A. ilicifolius had the highest proportional cross-sectional area of aerenchyma air spaces, suggesting that the internal oxygen transfer to root tip was the fastest. However, the area of aerenchyma air spaces in the root tip of 10NW treated A. ilicifolius was significantly reduced while area of epidermis and hypodermis (E + H) increased leading to less oxygen supply to root tip. Compared to B. gymnorrhiza and E. agallocha, the (E + H) layer of A. ilicifolius was the thinnest, and the cells without suberized walls were loosely packed in all three treatments. These results suggested that the root anatomy and ROL of B. gymnorrhiza was least affected by wastewater discharge, followed by E. agallocha, and A. ilicifolius was the most susceptible species thus was not suitable for treating strong wastewater.

show abstract

Exploring the Radial and Longitudinal Aeration of Primary Maize Roots by Means of Clark-Type Oxygen Microelectrodes

Cited by 30 publications

References 32 publications

Plant Anaerobic Stress as a Novel Trend in Ecological Physiology, Biochemistry, and Molecular Biology: 1. Establishment of a New Scientific Discipline

Plant Anaerobic Stress as a Novel Trend in Ecological Physiology, Biochemistry, and Molecular Biology: 1. Establishment of a New Scientific Discipline

Self-referencing optrodes for measuring spatially resolved, real-time metabolic oxygen flux in plant systems

Effect of Wastewater Discharge on Root Anatomy and Radial Oxygen Loss (ROL) Patterns of Three Mangrove Species in Southern China

Contact Info

Product

Resources

About