Exploring the Potential of Alginate-Gelatin-Diethylaminoethyl Cellulose-Fibrinogen based Bioink for 3D Bioprinting of Skin Tissue Constructs

Ramakrishnan, Rashmi; Kasoju, Naresh; Raju, Riya; Geevarghese, Rency; Gauthaman, Ashna; Bhatt, Anugya

doi:10.1016/j.carpta.2022.100184

Cited by 16 publications

(25 citation statements)

References 40 publications

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“…Nevertheless, only osteogenic-specific genes were studied. Finally, a comparable bioink formulation of 2.0% alginate, 3.3% gelatin, and 0.93% diethylaminoethyl cellulose was used for skin bioprinting, yielding promising results [ 69 , 70 ] . These studies suggest that the proposed bioink could be used for other 3D bioprinting applications.…”

Section: Discussionmentioning

confidence: 99%

Formulation and evaluation of a bioink composed of alginate, gelatin, and nanocellulose for meniscal tissue engineering

Semba¹,

Mieloch²,

Tomaszewska³

et al. 2022

IJB

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The necessity to preserve meniscal function prompts the research and develop¬ment of novel treatment options, like three-dimensional (3D) bioprinting. However, bioinks for meniscal 3D bioprinting have not been extensively explored. Therefore, in this study, a bioink composed of alginate, gelatin, and carboxymethylated cellu¬lose nanocrystal (CCNC) was formulated and evaluated. Firstly, bioinks with varying concentrations of the aforementioned components were subjected to rheological analysis (amplitude sweep test, temperature sweep test, and rotation). The optimal bioink formulation of 4.0% gelatin, 0.75% alginate, and 1.4% CCNC dissolved in 4.6% D-mannitol was further used for printing accuracy analysis, followed by 3D bioprint¬ing with normal human knee articular chondrocytes (NHAC-kn). The encapsulated cells’ viability was > 98%, and collagen II expression was stimulated by the bioink. The formulated bioink is printable, stable under cell culture conditions, biocompatible, and able to maintain the native phenotype of chondrocytes. Aside from meniscal tissue bioprinting, it is believed that this bioink could serve as a basis for the devel¬opment of bioinks for various tissues.

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Section: Discussionmentioning

confidence: 99%

Formulation and evaluation of a bioink composed of alginate, gelatin, and nanocellulose for meniscal tissue engineering

Semba¹,

Mieloch²,

Tomaszewska³

et al. 2022

IJB

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“…A standard (ISO 10993-part 4: in vitro blood compatibility of biomaterials) hemolysis assay percentage (%) was employed to screen for the hemocompatibility of the SFFIBHA scaffold. For topical use of the scaffold as an artificial skin substitute, other extensive hemocompatibility tests are not indicated . Briefly, the freeze-dried scaffolds (10 mm disk) were hydrated with PBS, followed by incubation in anticoagulated blood with gentle shaking at 70 ± 5 rpm (Shaker, Lab-line Instruments Inc.) at 35 ± 2 °C.…”

Section: Methodsmentioning

confidence: 99%

“…For topical use of the scaffold as an artificial skin substitute, other extensive hemocompatibility tests are not indicated. 32 Briefly, the freeze-dried scaffolds (10 mm disk) were hydrated with PBS, followed by incubation in anticoagulated blood with gentle shaking at 70 ± 5 rpm (Shaker, Labline Instruments Inc.) at 35 ± 2 °C. The total hemoglobin in the blood samples was estimated using an automatic hematology analyzer (ERBA H360, Germany).…”

Section: Validation Of Terminal Sterilization For the Sffibha Scaffoldmentioning

confidence: 99%

Silk Fibroin-Based Bioengineered Scaffold for Enabling Hemostasis and Skin Regeneration of Critical-Size Full-Thickness Heat-Induced Burn Wounds

Ramakrishnan

Chouhan

Sreelatha

et al. 2022

ACS Biomater. Sci. Eng.

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Millions of people around the globe are affected by full-thickness skin injuries. A delay in the healing of such injuries can lead to the formation of chronic wounds, posing several clinical and economic challenges. Current strategies for wound care aim for skin regeneration and not merely skin repair or faster wound closure. The present study aimed to develop a bioactive wound-healing matrix comprising natural biomaterial silk fibroin (SF), clinical-grade human fibrin (FIB), and human hyaluronic acid (HA), resulting in SFFIBHA for regeneration of full-thickness burn wounds. A porous, hemostatic, self-adhesive, moisture-retentive, and biomimetic scaffold that promotes healing was the expected outcome. The study validated a terminal sterilization method, suggesting the stability and translational potential of the novel scaffold. Also, the study demonstrated the regenerative abilities of scaffolds using in vitro cell culture experiments and in vivo full-thickness burn wounds of critical size (4 cm × 4 cm) in a rabbit model. Under in vitro conditions, the scaffold enhanced primary dermal fibroblast adhesion and cell proliferation with regulated extracellular matrix (ECM) synthesis. In vivo, the scaffolds promoted healing with mature epithelium coverage involving intact basal cells, superficial keratinocytes, multilayers of keratohyalin, dermal regeneration with angiogenesis, and deposition of remodeled ECM in 28 days. The relative gene expression of the IL6 marker indicated transitions from inflammation to proliferation stage. In addition, we observed skin appendages and rete peg development in the SFFIBHA-treated wound tissues. Although wound closure was observed, neither negative (untreated/sham) nor positive (commercially available product; NeuSkin) control wounds developed skin appendages/rete pegs or native skin architecture. After 56 days, healing with organized ECM production enabled the recovery of mechanical properties of skin with higher tissue maturity in SFFIBHA-treated wounds. Thus, in a single application, the SFFIBHA scaffold proved to be an efficient biomimetic matrix that can guide burn wound regeneration. The developed matrix is a suture-less, hemostatic, off-the-shelf product for potential wound regenerative applications.

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“…Artificial bionic skin prepared by 3D bioprinting technology is a better coping strategy ( Kim et al, 2020 ; Jin et al, 2021 ). Ramakrishnan et al (2022) optimized the formulation and developed alginate, gelatin, diethylaminoethylcellulose and fibrinogen, a bioink that used the RegenHu 3D Discovery Bioprinter to establish excellent printability, shape fidelity, and cell-laden tissue-equivalent printing. Human primary fibroblasts and keratinocyte-loaded bioprinted constructs exhibited good cell viability.…”

Section: Application Of Extracellular Matrix-based Bioprinting Materi...mentioning

confidence: 99%

An Overview of Extracellular Matrix-Based Bioinks for 3D Bioprinting

Wang

Zhou

et al. 2022

Front. Bioeng. Biotechnol.

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As a microenvironment where cells reside, the extracellular matrix (ECM) has a complex network structure and appropriate mechanical properties to provide structural and biochemical support for the surrounding cells. In tissue engineering, the ECM and its derivatives can mitigate foreign body responses by presenting ECM molecules at the interface between materials and tissues. With the widespread application of three-dimensional (3D) bioprinting, the use of the ECM and its derivative bioinks for 3D bioprinting to replicate biomimetic and complex tissue structures has become an innovative and successful strategy in medical fields. In this review, we summarize the significance and recent progress of ECM-based biomaterials in 3D bioprinting. Then, we discuss the most relevant applications of ECM-based biomaterials in 3D bioprinting, such as tissue regeneration and cancer research. Furthermore, we present the status of ECM-based biomaterials in current research and discuss future development prospects.

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Exploring the Potential of Alginate-Gelatin-Diethylaminoethyl Cellulose-Fibrinogen based Bioink for 3D Bioprinting of Skin Tissue Constructs

Cited by 16 publications

References 40 publications

Formulation and evaluation of a bioink composed of alginate, gelatin, and nanocellulose for meniscal tissue engineering

Formulation and evaluation of a bioink composed of alginate, gelatin, and nanocellulose for meniscal tissue engineering

Silk Fibroin-Based Bioengineered Scaffold for Enabling Hemostasis and Skin Regeneration of Critical-Size Full-Thickness Heat-Induced Burn Wounds

An Overview of Extracellular Matrix-Based Bioinks for 3D Bioprinting

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