Exploring the persistence and spreading of antibiotic resistance from manure to biocompost, soils and vegetables

Pu, Chengjun; Yao, Yu; Diao, Jian-Xiong; Gong, Xiaoyan; Li, Ji; Sun, Ying

doi:10.1016/j.scitotenv.2019.06.081

Cited by 67 publications

(32 citation statements)

References 65 publications

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“…Whole-genome sequencing identified the prevalence of bla NDM positive E. coli in farms, as well as in flies, dogs, and farmers, thus demonstrating that bla NDM can rapidly contaminate humans through dogs, flies, and wild birds. This provides direct evidence for the spread of carbapenem-resistant E. coli and environmental pollution [122]. ARBs and ARGs from organic fertilizers have been detected in crops grown on soil enriched with organic fertilizers [123].…”

Section: Gene Transfer Agentsmentioning

confidence: 86%

Antibiotic resistance genes in bacteria: Occurrence, spread, and control

et al. 2021

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The production and use of antibiotics are becoming increasingly common worldwide, and the problem of antibiotic resistance is increasing alarmingly. Drug-resistant infections threaten human life and health and impose a heavy burden on the global economy. The origin and molecular basis of bacterial resistance is the presence of antibiotic resistance genes (ARGs). Investigations on ARGs mostly focus on the environments in which antibiotics are frequently used, such as hospitals and farms. This literature review summarizes the current knowledge of the occurrence of antibiotic-resistant bacteria in nonclinical environments, such as air, aircraft wastewater, migratory bird feces, and sea areas in-depth, which have rarely been involved in previous studies.

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Section: Gene Transfer Agentsmentioning

confidence: 86%

Antibiotic resistance genes in bacteria: Occurrence, spread, and control

et al. 2021

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“…Various other studies reported that vegetables may become contaminated with multidrug resistant bacteria from soil, manure fertilization, irrigation water or through direct contamination by humans [2]. In this context, the major way in which antibiotic resistance enters the soil is through the use of animal manure [71]. In Australia, Zhang et al explored the impact of cattle and poultry manure application on the resistome in lettuce and soil microbiomes, including the rhizosphere, root endosphere, leaf endosphere and phyllosphere.…”

Section: Contamination Pathways and Genetic Characteristics Of β β β-Lactamases And Mcr-producing Gram-negative Bacteriamentioning

confidence: 99%

Vegetables and Fruit as a Reservoir of β-Lactam and Colistin-Resistant Gram-Negative Bacteria: A Review

et al. 2021

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Antibacterial resistance is one of the 2019 World Health Organization’s top ten threats to public health worldwide. Hence, the emergence of β-lactam and colistin resistance among Gram-negative bacteria has become a serious concern. The reservoirs for such bacteria are increasing not only in hospital settings but in several other sources, including vegetables and fruit. In recent years, fresh produce gained important attention due to its consumption in healthy diets combined with a low energy density. However, since fresh produce is often consumed raw, it may also be a source of foodborne disease and a reservoir for antibiotic resistant Gram-negative bacteria including those producing extended-spectrum β-lactamase, cephalosporinase and carbapenemase enzymes, as well as those harboring the plasmid-mediated colistin resistance (mcr) gene. This review aims to provide an overview of the currently available scientific literature on the presence of extended-spectrum β-lactamases, cephalosporinase, carbapenemase and mcr genes in Gram-negative bacteria in vegetables and fruit with a focus on the possible contamination pathways in fresh produce.

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“…In the TH-MAD plant, tetracycline and glycopeptide resistance genes are also enriched, as are the MGEs tnpA (IS6 family) and IS613. IS613 was also enriched during the temperature decreasing stage of composting [36] and has been found to be associated with ARGs [37,38].…”

Section: Fate Of Args and Mges During Th-mad And Madmentioning

confidence: 96%

Fate of antibiotic resistant E. coli and antibiotic resistance genes during full scale conventional and advanced anaerobic digestion of sewage sludge

et al. 2020

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Antibiotic resistant bacteria (ARB) and their genes (ARGs) have become recognised as significant emerging environmental pollutants. ARB and ARGs in sewage sludge can be transmitted back to humans via the food chain when sludge is recycled to agricultural land, making sludge treatment key to control the release of ARB and ARGs to the environment. This study investigated the fate of antibiotic resistant Escherichia coli and a large set of antibiotic resistance genes (ARGs) during full scale anaerobic digestion (AD) of sewage sludge at two U.K. wastewater treatment plants and evaluated the impact of thermal hydrolysis (TH) pre-treatment on their abundance and diversity. Absolute abundance of 13 ARGs and the Class I integron gene intI1 was calculated using single gene quantitative (q) PCR. High through-put qPCR analysis was also used to determine the relative abundance of 370 ARGs and mobile genetic elements (MGEs). Results revealed that TH reduced the absolute abundance of all ARGs tested and intI1 by 10–12,000 fold. After subsequent AD, a rebound effect was seen in many ARGs. The fate of ARGs during AD without pre-treatment was variable. Relative abundance of most ARGs and MGEs decreased or fluctuated, with the exception of macrolide resistance genes, which were enriched at both plants, and tetracyline and glycopeptide resistance genes which were enriched in the plant employing TH. Diversity of ARGs and MGEs decreased in both plants during sludge treatment. Principal coordinates analysis revealed that ARGs are clearly distinguished according to treatment step, whereas MGEs in digested sludge cluster according to site. This study provides a comprehensive within-digestor analysis of the fate of ARGs, MGEs and antibiotic resistant E. coli and highlights the effectiveness of AD, particularly when TH is used as a pre-treatment, at reducing the abundance of most ARGs and MGEs in sludgeand preventing their release into the environment.

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Exploring the persistence and spreading of antibiotic resistance from manure to biocompost, soils and vegetables

Cited by 67 publications

References 65 publications

Antibiotic resistance genes in bacteria: Occurrence, spread, and control

Antibiotic resistance genes in bacteria: Occurrence, spread, and control

Vegetables and Fruit as a Reservoir of β-Lactam and Colistin-Resistant Gram-Negative Bacteria: A Review

Fate of antibiotic resistant E. coli and antibiotic resistance genes during full scale conventional and advanced anaerobic digestion of sewage sludge

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