Exploring the interactions between protein coronated CdSe quantum dots and nanoplastics

Chakraborty, Debolina; Naik, Sanjay; Kumar, Sanjit; Chandrasekaran, N.; Mukherjee, Amitava

doi:10.1039/d1nj00441g

Cited by 7 publications

(7 citation statements)

References 37 publications

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“…Larger agglomerated protein corona structures were also formed as a result of micron‐sized cluster formation of CdS–GT QDs with HSA in biological fluids. [ 50 ]…”

Section: Resultsmentioning

confidence: 99%

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Interaction properties of biosynthesized cadmium sulphide quantum dots with human serum albumin: further investigation of antibacterial activities and sensing applications

2022

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The synthesis of small-sized quantum dots (QDs) (1-10 nm) via the green route has garnered great interest regarding their prospective use in many biological applications (diagnosis, drug delivery and in vivo sensing); this is difficult to achieve using chemical synthesis methods, which produce larger size QD particles and also require hazardous reagents. Here, we synthesized biogenic cadmium sulphide (CdS) QDs using green tea extract as the reducing agent to produce particles that were homogeneous and a smaller size of 2-4 nm. We also elucidated the (a) protein binding, (b) antibacterial use and (c) sensing applications of biogenic CdS QDs in this present work. The biosynthesized CdS QDs were found to have extensive antibacterial activity against both Gram-negative Escherichia coli and Gram-positive Enterococcus faecalis bacterial strains. The introduction of QDs in biological medium can lead to the formation of protein-QD complexes; therefore we investigated the binding interaction of CdS QDs with the carrier protein human serum albumin (HSA) in vitro. The synthesized CdS QDs quenched the intrinsic fluorescence of HSA through a static quenching mechanism and the binding constant (K b ) was in the order of 10 4 M À1 . It was also observed that the presence of biogenic CdS QDs affected the HSA-ligand interactions in vitro. The synthesized CdS made highly effective sensors for tetracycline, rifampicin, and bilirubin with limit of detection (LOD) values of 99, 141 and 29 ng/ml, respectively.

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“…Larger agglomerated protein corona structures were also formed as a result of micron‐sized cluster formation of CdS–GT QDs with HSA in biological fluids. [ 50 ]…”

Section: Resultsmentioning

confidence: 99%

“…Larger agglomerated protein corona structures were also formed as a result of micron-sized cluster formation of CdS-GT QDs with HSA in biological fluids. [50] 3.3 | Fluorescence quenching and binding analysis of HSA in the presence of CdS-GT QDs/ligands…”

Section: Sensing Studiesmentioning

confidence: 99%

Interaction properties of biosynthesized cadmium sulphide quantum dots with human serum albumin: further investigation of antibacterial activities and sensing applications

2022

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“…Larger agglomerated protein-corona structures were also observed in the Figure S9, which was due to micron sized cluster formation of the respective CdSe QDs in the biological matrix. [55] Fluorescence response of CdSe QDs to lomefloxacin Fluorometry is the time and cost-effective technique used in detection of antibiotics based on fluorescence probe. Before performing the detection of lomefloxacin, we have investigated the optimized conditions.…”

Section: Uv-vis Absorption Studies For Ground-state Complex Formationmentioning

confidence: 99%

Binding of Water‐Soluble CdSe Quantum Dots with Human Serum Albumin: Further Studies into their Effects on Dietary Polyphenol Binding and Sensing of Antibiotic Lomefloxacin

et al. 2021

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Synthesis of surface modified quantum dots (QDs) having potential biological activities are great important in biomedicinal chemistry. Here, we have synthesized mercapto propanoic acid (MPA) and thiolactic acid (TLA) coated water soluble cadmium selenide (CdSe) QDs having biological activity. Dispersion of QDs into a biological fluid can form protein corona. Hence, the binding of CdSe QDs with the carrier protein, human serum albumin (HSA) have been executed using spectroscopic methods. The binding affinity order of CdSe QDs-HSA complexes were found in the order of 10 5 M À 1 at 298 K. CdSe QDs are also affects the binding affinities of dietary polyphenols (naringin: NG and naringenin: NRG) towards HSA. The synthesized CdSe QDs was employed to sensing of antibiotic lomefloxacin by fluorescence quenching mechanism. The detection limit was found 0.70-1.83 μg mL À 1 . The CdSe QDs also demonstrate the detection of lomefloxacin under uv light based on color change of the solutions.

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“…In addition to analyzing a contaminant‘s direct impact on cells, it is vital to identify dynamic interactions that are feasible in an in vivo system. In an initial study conducted previously by our group, it was observed that functionalized PS‐beads could alter the colloidal stability of coronated CdSe quantum dots (QDs) forming large agglomerated complexes [13] …”

Section: Introductionmentioning

confidence: 99%

“…In an initial study conducted previously by our group, it was observed that functionalized PS-beads could alter the colloidal stability of coronated CdSe quantum dots (QDs) forming large agglomerated complexes. [13] In the present study, we aim to elucidate the multilayer interaction pattern of GNRs from a mixture effect perspective. Considering the wide range of biomedical applications, GNRs were selected as a model nanoparticle.…”

Section: Introductionmentioning

confidence: 99%

Studying the Interaction Behavior of Protein Coronated Gold Nanorods with Polystyrene Nanoplastics

et al. 2022

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The formation of protein corona and their modulatory effects on the physicochemical and biological activity of the nanomaterial is well established. The active protein corona on the nanoparticles can further mediate interactions with trace amounts of exogenous entities present in the systemic circulation. In the present study, we utilize an in vitro simulation system wherein, protein corona is strategically engineered by varying the serum concentration on a model nanoparticle i. e. cationic‐gold nanorods (GNRs). Quantitative analysis indicated that the 4 nm cationic GNRs can accumulate as high as ∼1 mg/mL protein corona upon incubation with serum. The size distribution pattern of the bound corona and their subsequent impact on the physicochemical properties of GNRs were determined. To understand how these coronated nanorods would interact with a potent pollutant, the second layer of interaction was further introduced by interacting the coronated‐GNRs with 1 ppm nanoplastic. The impact of differently functionalized polystyrene beads (plain, aminated, and carboxylated) on the core size, hydrodynamic diameter, surface charge, and optical properties of the coronated GNRs is highlighted. A complex interplay of several interactive forces and amino acid‐mediated binding of the GNRs to the styrene and benzene rings of the nanoplastic leads to the formation of a coronated‐GNR‐PS complex (>400 nm). Further, upon subjecting the hybrid‐conjugate system to acidic pH conditions (pH‐5.6 and 4.3), a considerable increase in protein leaching was observed. The novel approach of the present study aims to look into the layered interactive patterns for nanomaterials that are utilized for in vivo applications.

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Exploring the interactions between protein coronated CdSe quantum dots and nanoplastics

Abstract: It is now established that the metastable colloidal nanoparticles (NPs) are subjected to interaction with proteins in biological media leading to the formation of protein corona on the NPs. The...

Cited by 7 publications

References 37 publications

Interaction properties of biosynthesized cadmium sulphide quantum dots with human serum albumin: further investigation of antibacterial activities and sensing applications

Interaction properties of biosynthesized cadmium sulphide quantum dots with human serum albumin: further investigation of antibacterial activities and sensing applications

Binding of Water‐Soluble CdSe Quantum Dots with Human Serum Albumin: Further Studies into their Effects on Dietary Polyphenol Binding and Sensing of Antibiotic Lomefloxacin

Studying the Interaction Behavior of Protein Coronated Gold Nanorods with Polystyrene Nanoplastics

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